Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,104,837	G→A	A83V (GCC→GTC)	wbbJ ←	putative lipopolysaccharide biosynthesis O‑acetyl transferase WbbJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,104,837	0	G	A	100.0%	59.2 / NA	19	A83V (GCC→GTC)	wbbJ	putative lipopolysaccharide biosynthesis O‑acetyl transferase WbbJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/9);  total (10/9)

ACAACAGAATTGGCGCCGACTACGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGTCCAAATGCATCCAGCCTGAGACCGACTCCACTTGTGAAATTTTCACCAAAATTAATGCTACCATCATTGCGAATATAGCAGGGAAATCGAATAATTCTACAGTTCCGGTAAAATACACGAGTCAATAAGACATCTCTAACAAGCCGAATAAAACCACAGAGACCATATCGTTTAGCGAGTTTTA  >  NC_000913/2104591‑2105077                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 acaacaGAATTGGCGCCGACTACGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGACGat                                                                                                                                                                                                                                              <  1:72244/251‑1 (MQ=255)           tGGCGCCGACTACGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGACGATATGAACAt                                                                                                                                                                                                                                      >  1:280505/1‑249 (MQ=255)             gcgcCGACTACGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGACGATATGAACATAggc                                                                                                                                                                                                                                  <  2:91492/251‑3 (MQ=255)                                              ccAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGACGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCAc                                                                                                                                                                                                 <  2:165393/251‑1 (MQ=255)                                               cAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGACGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACg                                                                                                                                                                                                >  2:281970/1‑251 (MQ=255)                                                    aaaaCCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAAAAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAAACGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACg                                                                                                                                                                                           <  2:12268/251‑1 (MQ=255)                                                               aTTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTACAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGACCCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGACGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGTCCAAATGCAt                                                                                                                                                                                >  1:170521/1‑251 (MQ=255)                                                                                                       gAAGATTTCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCATTTGCGACGATATGAACATAGTCGTTAACTTGCACATTATCGGACAAAAAAATCACGCAACGTCCAACTGCATCCAGCCTTAGACTGACTCCACTTGTGAAATTTTCACCaa                                                                                                                                         >  2:186/1‑250 (MQ=255)                                                                                                       gAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGACGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGTCCAAATGCATCCAGCCTGAGACCGACTCCACTTGTGAAATTTTCACCaaa                                                                                                                                        <  2:280505/251‑1 (MQ=255)                                                                                                                   gTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTAAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGACGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGTCCAAATGCATCCAGCCTGAGACCGACTCCACTTGTGAAATTTTCACCAAAATTAATGCTAcc                                                                                                                            <  2:85208/251‑1 (MQ=255)                                                                                                                                          tGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGACGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGTCCAAATGCATCCAGCCTGAGACCGACTCCACTTGTGAAATTTTCACCAAAATTAATGCTACCATCATTGCGAATATAGCAGGGa                                                                                                      <  1:281970/250‑1 (MQ=255)                                                                                                                                                                 gCTTAAAGAAACCGTGATTATGATCTGTAATAAATACTTTCCTAGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGACGATATGAACATAGTCGTTAACTTTCACATTATCGGAAAAAAAAATCACGCCACGTCCAAATGCATCCAGCCTGAGACCGACTCCACTTGTGAAATTTTCACCAAAATTAATGCTACCATCATTGCGAATATAGCAGGGAAATCGAATAATTCTACAGTTCCg                                                                               <  1:172968/250‑1 (MQ=255)                                                                                                                                                                             cGTGAGTATGAGCGGTAATAAATACTTTACTTGCAATAAGCGTATACCGACATACAGAAACGCTCACACTTGAGACGATATGAACATAGTCGTTCACTTGCCCATTATCGGAAAAAAAAATCACGACACGTCCTAATGCATCCAGACTGAGACCAACTCACCTTGTGAACTTTTCACCAAAATTAATGCTACCATCATTGCGCATATAGCAGGGAAATCAAATAATTTTACAGTTCCGGTAAAATACACg                                                                   >  2:270298/1‑250 (MQ=255)                                                                                                                                                                               tGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGACGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGTCCAAATGCATCCAGCCTGAGACCGACTCCACTTGTGAAATTTTCACCAAAATTAATGCTACCATCATTGCGAATATAGCAGGGAAATCGAATAATTCTACAGTTCCGGTAAAATACACGAGt                                                                >  1:250612/1‑251 (MQ=255)                                                                                                                                                                                  ttATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGACGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGTCCAAATGCATCCAGCCTGAGACCGACTCCACTTGTGAAATTTTCACCAAAATTAATGCTACCATCATTGCGAATATAGCAGGGAAATCGAATAATTCTACAGTTCCGGTAAAATACACGAGTCa                                                              >  1:82347/1‑250 (MQ=255)                                                                                                                                                                                        tCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGACGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGTCCAAATGCATCCAGCCTGAGACCGACTCCACTTGTGAAATTTTCACCAAAATTAATGCTACCATCATTGCGAATATAGCAGGGAAATCGAATAATTCTACAGTTCCGGTAAAATACACGAGTCAATAAGAc                                                       >  2:78872/1‑251 (MQ=255)                                                                                                                                                                                            taagaaatgCTTTACATACAATAAGCGTCTCCAGACATATGGCAACGCTAACAATTGAGACGATATGAAAATAGTCGTTCACTTGCACATTATCGGAAAACAAAATCACGCCACGTCCAAATGCATCCAGCCTGAGACCGACTCCACTTGTGAAATTTTCACCAAAATTAATGCTACCATCATTGCGACTATAGCAGGGAAATCGAATAATTCTACAGTTCCGGTAAAATACACGAGTCAATAAGACAtat                                                   >  2:128290/5‑249 (MQ=255)                                                                                                                                                                                                                        aTCCGGACCTATCGTAACGCTCTCAATGGAGACGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAATATCACGCCACGTCCAAATGCATCCAGCCTGAGACCGACTCCACGTGTGAAATTTTCACCAAAATTAATGCTACCATCATTGCGAATATAGCAGGGAAATCGAATAATTCTACAGTTCCGGTAAAATACACGAGTCAATAAGACATCTCTAACAAGCCGAATAAAACCACAGAGAc                       <  1:42129/251‑1 (MQ=255)                                                                                                                                                                                                                                                  ttGAGACGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGTCCAAATGCATCCAGCCTGAGACCGACTCCACTTGTGAAATTTTCACCAAAATTAATGCTACCATCATTGCGAATATAGCAGGGAAATCGAATAATTCTACAGTTCCGGTAAAATACACGAGTCAATAAGACATCTCTAACAAGCCGAATAAAACCACAGAGACCATATCGTTTAGCGAGTTTTa  >  2:184058/1‑246 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 ACAACAGAATTGGCGCCGACTACGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGTCCAAATGCATCCAGCCTGAGACCGACTCCACTTGTGAAATTTTCACCAAAATTAATGCTACCATCATTGCGAATATAGCAGGGAAATCGAATAATTCTACAGTTCCGGTAAAATACACGAGTCAATAAGACATCTCTAACAAGCCGAATAAAACCACAGAGACCATATCGTTTAGCGAGTTTTA  >  NC_000913/2104591‑2105077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑