Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,353,758	T→A	D254V (GAT→GTT)	sapC ←	putrescine ABC exporter membrane protein SapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,353,758	0	T	A	100.0%	104.8 / NA	31	D254V (GAT→GTT)	sapC	putrescine ABC exporter membrane protein SapC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/14);  total (17/14)

CGGATTTCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATAC  >  NC_000913/1353522‑1354004                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       cgGATTTCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCt                                                                                                                                                                                                                                          >  2:88669/1‑251 (MQ=255)      ttCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACACTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTcccc                                                                                                                                                                                                                                      <  2:71470/250‑1 (MQ=255)                      gTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGt                                                                                                                                                                                                                     <  1:154174/251‑1 (MQ=255)                                  cGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCg                                                                                                                                                                                                            >  1:69868/1‑248 (MQ=255)                                   ggTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCg                                                                                                                                                                                                            >  1:164856/1‑247 (MQ=255)                                        aCGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCgaga                                                                                                                                                                                                   <  1:206817/251‑1 (MQ=255)                                                       tcatcaCCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCgcggcg                                                                                                                                                                                    >  1:46366/1‑251 (MQ=255)                                                          acaCCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGata                                                                                                                                                                                 <  2:200783/250‑1 (MQ=255)                                                           caCCGGTTTTAAATTAAATAGTCCGATTACGAATAACGAGTAATGACATTATTCCACCTCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCGGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCCCAACACCGAGCATCGCTCCCCATTCAGTACAGTGGAGTTGTGCGCCGCGATCGAGAACGCCCAGCgcggacatat                                                                                                                                                                                >  2:37154/1‑245 (MQ=255)                                                             ccGGTTTTAAATTCAATGGGCATGTTACGAATATCGAGTAATGGCATTATTCCACCCCCCCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCGCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCTATATCg                                                                                                                                                                              <  2:160737/251‑1 (MQ=255)                                                              cGGTTTTACATTCAACGGAAAGGTTAAGAATATCGAGTACTGGCATAATTCCACCCCCTCAATAATCGCACGACGCACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGCCAGTCCACAGGGCGACATAAATCAGTTCCAGCGCAACACCAAGCATCGCTCCCCATTCATCCGAGGAGAGCTGTGCGCCGAGATGGAGAAACCCCAGCGCGGCGCTATCgc                                                                                                                                                                             >  2:26888/1‑250 (MQ=255)                                                              cGGTTTTAAATTCAATGGTCAGGTTACGAACATCGAGTAATGGCATTATTCCACCCCCGCAAGAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCGGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCGCCCCAGTCAGGCGAGGGGAGATGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCga                                                                                                                                                                             <  2:106317/251‑1 (MQ=255)                                                               ggTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCgag                                                                                                                                                                            <  1:137231/251‑1 (MQ=255)                                                                   ttAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCGGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAAtt                                                                                                                                                                        >  1:41164/1‑251 (MQ=255)                                                                       aTTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCa                                                                                                                                                                    <  1:9790/251‑1 (MQ=255)                                                                                       aCGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCggg                                                                                                                                                     <  2:69868/250‑1 (MQ=255)                                                                                                  gTAATGGCATTCTTCCATCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCCACACCGAGCATCGCTCCCCATTCAGGCGAGGGGCGTTGTGCGCCGAGATCGACAAAACCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTg                                                                                                                                          >  2:111402/1‑250 (MQ=255)                                                                                                            tattCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGTGGACCAGCCcgg                                                                                                                                <  2:161822/250‑1 (MQ=255)                                                                                                             attCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCcgg                                                                                                                                >  1:53492/1‑249 (MQ=255)                                                                                                                  aCCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCAcggcgggg                                                                                                                           >  2:84926/1‑247 (MQ=255)                                                                                                                        gCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATAATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGCGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTgg                                                                                                                   >  2:238456/1‑251 (MQ=255)                                                                                                                                  cACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTAAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGGTCTAGGTGACCAGCCCGGCGGAGATGTTTGGCATCACAGc                                                                                                          >  1:259091/1‑250 (MQ=255)                                                                                                                                          aCGCCATCGCCAAGGAGGTTAACCACCATCACGCTAATCAGAATTGCCGCTCCTGGCAGCATGGCAGTCCTCGGGGCGACAGAAATCAGTTCCAGCGCAACACCTAGCATCGCTCCCCATGCAGGCGAGGGGAGTTGTGCGCCTAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCGATCGTCGGTGCTCGGGTGATCTCCGTGGCCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCagag                                                                                                 <  2:28105/251‑1 (MQ=255)                                                                                                                                                                       cacGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGAc                                                                    <  1:248610/251‑1 (MQ=255)                                                                                                                                                                         cGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACgg                                                                  >  1:152560/1‑251 (MQ=255)                                                                                                                                                                                                      aTGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCAAAGCAAACCAGAGAATAATCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCCGTTc                                     >  1:260353/1‑251 (MQ=255)                                                                                                                                                                                                                   gggCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATg                        <  2:87228/251‑1 (MQ=255)                                                                                                                                                                                                                       gACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGGGATGTTTGGCATCACAGCAAAACAGAGAATATTACGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCCTGCACCATGCTGt                    >  2:230141/1‑251 (MQ=255)                                                                                                                                                                                                                             aaTCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCg              >  1:156895/1‑251 (MQ=255)                                                                                                                                                                                                                             aaTCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGAGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCg              >  1:43742/1‑251 (MQ=255)                                                                                                                                                                                                                                         cgcAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATAc  <  1:4640/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       CGGATTTCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATAC  >  NC_000913/1353522‑1354004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑