Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,609,197 C→T F237F (TTC→TTT)  hyfG → hydrogenase 4 catalytic subunit HyfG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,609,1970CT96.0% 74.8 / NA 25F237F (TTC→TTT) hyfGhydrogenase 4 catalytic subunit HyfG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/15);  minor base G (0/1);  total (9/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTCTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCGATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAAT  >  NC_000913/2608967‑2609402
                                                                                                                                                                                                                                      |                                                                                                                                                                                                             
cgcCCTGCACCAACGACTGATTCCGAACTGTATCCGTTAAGCAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGGCCCGCTGGATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGGAAATTGTCGATGCTGATTACCGCCTGTTTTATGGCCATCGCGGCATGGAGAAACTGGCAGAAACGCGTATGGGCTACAACGAAGTGACCTTTTTATCGGACTGCGTGTGTgg                                                                                                                                                                                           <  2:22616/251‑1 (MQ=255)
cgcCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACTAAGTGACCTTTTTATCGGACCGCGTGTGTgg                                                                                                                                                                                           <  1:53458/251‑1 (MQ=255)
     tGAACCGACGACTGATTCCGAAACGTATCCGTTTATAAATGAGGGCAACAGCGATGCGAGGGTGATCACTGTCGGCCCGCTGCATATCACCTCCGATGAACAGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGAGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAAAGAAGTGACCTTTTTCTCGGACCGCGTGTGTGGGAttt                                                                                                                                                                                      >  2:263522/1‑251 (MQ=255)
          cgacgaATGATTCCGAAACGTATGCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGGCCCGCCGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGTGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGCGACCTTTTTATCGGCCCGCGTGTGTGGGATTTGCGGt                                                                                                                                                                                 >  2:173738/1‑251 (MQ=255)
             cgacTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTTTGTGGGATTTGCGGtttt                                                                                                                                                                              <  1:209747/251‑1 (MQ=255)
             cgacTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGtttt                                                                                                                                                                              <  1:229099/251‑1 (MQ=255)
                 tGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGccc                                                                                                                                                                          >  2:106134/1‑251 (MQ=255)
                  gATTCCGAAACGTATCCGTTTATAAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGAGGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCca                                                                                                                                                                         <  2:198926/251‑1 (MQ=255)
                      ccGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGGCCCGCTGCATATCCCCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTGTTGCCCACAgt                                                                                                                                                                     <  1:238351/251‑1 (MQ=255)
                                      tatcaacaaGGGCAACAGCGAGGCGCGGGTGATCCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCAATTCCGCTTGTTTGTGGATGGCGAGCACATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACCGGCAGAAACGCGGATGGGCTTCAACGAAGTGACCTTTTTATCGGACCGCGTCTGTGGGATTTGCGGTTTTGCCCACAGTGTCGCTTATACCAAtt                                                                                                                                                     >  2:230851/9‑251 (MQ=255)
                                                  cAACAGCTATGCGCGGGTCATCCCTGTCGGCCCGCTACATATCACCTCGGATGAACCGGATCACTTCTGCTTGTTTGTGGATGCCGTGCAAATTGTCGATGCTGATTGCCGCCTGTTTTATGGCCATCGCCGCATGGAGAAACTGGCAGAAACGCGGATGGGATACAACGAAGTGACCTTGTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAACTCGGTTGAAAATg                                                                                                                                         <  2:24912/251‑1 (MQ=255)
                                                    ccAGCGATGCGCGGGTGATCCCTGTCGTCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCTCTTGTTTGTGGATGGCGAGCAAATTTTCGATGCTGATTTCCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCTGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCa                                                                                                                                       <  2:183826/250‑1 (MQ=255)
                                                           ggcgcGGGTGATCCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGa                                                                                                                                <  2:101100/250‑1 (MQ=255)
                                                                          tGTCGGCACGCTGCATCTCACCTCCGTTGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAATTTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGTGGCCGCAAc                                                                                                                 >  2:47541/1‑251 (MQ=255)
                                                                                cccGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGACAATGCACTGGGGATTGAGGTGCCGCAACGAGcac                                                                                                           >  2:57446/1‑251 (MQ=255)
                                                                                               cTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCg                                                                                             >  2:16654/1‑250 (MQ=255)
                                                                                                 ccGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCGAtt                                                                                          <  1:189175/251‑1 (MQ=255)
                                                                                                    aTGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCGATTctg                                                                                       <  2:191466/251‑1 (MQ=255)
                                                                                                           gggTCACTTCCGCGTGTTTGTGGATGGCGAGAAAATTGTCGATGCTGATTACCGCCTGGTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCGATTCTGCTGGAAg                                                                                <  2:104153/251‑1 (MQ=255)
                                                                                                               cACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCACCGAGCACATACTATTCGCTCGATTCTGCTGGAAGTCg                                                                             >  2:123594/1‑250 (MQ=255)
                                                                                                                                           aaaTTGTCGATGCTGATTACCGCCTGTTTTATGCCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCGATTCTTCTGGAAGTCGAACGGCTTCACAGTCATTTGCTTAACCtt                                                <  1:94993/251‑1 (MQ=255)
                                                                                                                                                             aCCGCCTGTTTTATGTCCAGCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATCCCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCGATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCAttt                               <  1:82047/250‑1 (MQ=255)
                                                                                                                                                                    gTTTTATGTCCATCTCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGATGTGACCTTTTTATCGTTCCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGCGTGGCCTATATCAATTCGGTTGAATATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCGATTCTGCTGGAAGTCTAGCGGCTACACAGTCATTTGCTTAGCCTTGGCCTCTCCTGCCATTTCGTTGGtt                       <  1:270639/251‑1 (MQ=255)
                                                                                                                                                                         aTNTCCATCGCGGCATGGAGAAACGGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATGCTAGTCTCTCGATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCCCCTGCCATTTCGTTGGTTTTGa                   <  2:254543/250‑1 (MQ=255)
                                                                                                                                                                                         ggAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTTTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCGATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAAt  >  1:137722/1‑251 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                             
CGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTCTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCGATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAAT  >  NC_000913/2608967‑2609402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑