Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,954,014	C→T	S179S (TCC→TCT)	ilvD →	dihydroxy‑acid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,954,014	0	C	T	100.0%	134.9 / NA	39	S179S (TCC→TCT)	ilvD	dihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/21);  total (18/21)

GAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATATCG  >  NC_000913/3953769‑3954255                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  gAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCagag                                                                                                                                                                                                                                              <  1:239995/251‑1 (MQ=255)        tCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATc                                                                                                                                                                                                                                         >  1:251577/1‑249 (MQ=255)          gCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGACGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGt                                                                                                                                                                                                                                     >  2:49051/1‑251 (MQ=255)                     tGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCACTGCGCCTGAATATTCCGGGGATCTTTGTATCCGGCGGCCCGATGGAGGCCGGGAAGACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCcg                                                                                                                                                                                                                          >  1:66546/1‑251 (MQ=255)                                   aCGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGc                                                                                                                                                                                                            >  2:224845/1‑251 (MQ=255)                                               ccGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTcc                                                                                                                                                                                                <  1:279092/251‑1 (MQ=255)                                                cGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCg                                                                                                                                                                                               <  1:120761/251‑1 (MQ=255)                                                   cGCCATGGTCTGCATCTCTAACTGCGACAAAATCCCCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGa                                                                                                                                                                                            <  1:284543/251‑1 (MQ=255)                                                         ggTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGttt                                                                                                                                                                                       >  1:274357/1‑250 (MQ=255)                                                                    ctAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTc                                                                                                                                                                            >  1:21460/1‑250 (MQ=255)                                                                                  aTCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGAc                                                                                                                                                             <  1:18269/251‑1 (MQ=255)                                                                                                   gATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCt                                                                                                                                              <  1:197197/249‑1 (MQ=255)                                                                                                          tCCCTGCGCCTGAATAGTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCTATCAGGTTGAACGTTCCGCGTGTCCGACCTGCTGTTCCTGGTCCGGGAGGGTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCGGGGCCTGCCGCAg                                                                                                                                       <  2:265914/249‑1 (MQ=255)                                                                                                               gcgcCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCggg                                                                                                                                  <  2:47421/249‑1 (MQ=255)                                                                                                                               gTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCACGCTGCTGGc                                                                                                                >  2:51603/1‑251 (MQ=255)                                                                                                                                      ttGTTTCCGGCGGCCCGATGGAGGCCGGTAAAACCAAACGTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAAcc                                                                                                            <  2:11938/248‑1 (MQ=255)                                                                                                                                      ttGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTAACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCAc                                                                                                         >  2:69936/1‑251 (MQ=255)                                                                                                                                         tttCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGcc                                                                                                      >  1:208532/1‑251 (MQ=255)                                                                                                                                                         ggAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGt                                                                                      >  1:123290/1‑251 (MQ=255)                                                                                                                                                             gacGGGAAAACCAAACTTGCCGATAAGATCAGCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTGCCGCGTGTCCGACCGGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACAGTAAGCAGCTGTTCCt                                                                                  <  2:201966/249‑1 (MQ=255)                                                                                                                                                                 ggAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAAt                                                                              >  1:179349/1‑251 (MQ=255)                                                                                                                                                                    aaaCCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCt                                                                           >  1:75266/1‑251 (MQ=255)                                                                                                                                                                    aaaCCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCt                                                                           >  1:276414/1‑251 (MQ=255)                                                                                                                                                                         aaaCTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGAGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGATGGTa                                                                       >  1:56588/1‑250 (MQ=255)                                                                                                                                                                           aCTNTCCGATAAGATCATCAAGCGCTATCTGGTTGATGCTATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACTGTCCGCGTGTCCTACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTTCCTGACCGAAGCGCTGTGCCTGTCGCAGCCGGGCAACTGCTCGCTGCTGGCATCCCACGCCGACCGTAATCAGCTGTGCCGTAATTCTGGTAAAc                                                                    <  2:254522/251‑1 (MQ=255)                                                                                                                                                                                 cgaacagatcaTCAAGCTCGATCTGGTTGATGCTATGATCCAGGCAGCCGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGGCCGACCTGCGTTTCCTGCTCCGGGATGTTTTTCGCTATCTCAATGAACCGCCTGACCGAAGCGCTGGGCTTGTCGCAGCCTGTCAACGGCTCGCTGCTGTCAACCCACGCCGACCGTAATCAGCTGTTCCTTAATCCTGTTTACCGCAttg                                                              <  2:14466/247‑1 (MQ=255)                                                                                                                                                                                 cGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCAttg                                                              <  1:48911/251‑1 (MQ=255)                                                                                                                                                                                           atcaAGCTCGCTCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGCAAACGCATTGTTGAAttacc                                                    >  2:28676/1‑248 (MQ=255)                                                                                                                                                                                               aGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCaaa                                                <  1:73769/251‑1 (MQ=255)                                                                                                                                                                                                   cGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTGCCGCGTGTCCGCCCTGCGGTTCCTGCTCCGGGATGGTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGGTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGt                                             <  2:85343/250‑1 (MQ=255)                                                                                                                                                                                                         ggTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGttat                                          <  1:241400/247‑1 (MQ=255)                                                                                                                                                                                                             gattcGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGc                                   >  2:26207/5‑250 (MQ=255)                                                                                                                                                                                                                 cGATGGTCCAGGGCGCATACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGCCCTGCGGTTCCTGCGCCGTGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGAGCCTGTCGCAGCCGTGCAACGGCTCGCTGCTAGCAACCCTCGCCTACCGTAAGCATCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAac                              <  1:160315/251‑1 (MQ=255)                                                                                                                                                                                                                               gcAGACCCGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACt                <  1:224845/251‑1 (MQ=255)                                                                                                                                                                                                                                    cccGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGa                                                                                                                                                                       >  2:19807/1‑95 (MQ=255)                                                                                                                                                                                                                                    cccGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGa                                                                                                                                                                       <  1:19807/95‑1 (MQ=255)                                                                                                                                                                                                                                      cGAAAGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGt         >  1:54202/1‑251 (MQ=255)                                                                                                                                                                                                                                          aGTATCTGACTCTCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCATCCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAata     <  1:49051/251‑1 (MQ=255)                                                                                                                                                                                                                                             agcTGACTCTCAGAGCGATCAGGTTGAACGTGCCGCGTGTCCAACCGGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATATCg  <  1:94170/249‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  GAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCAGATCATCAAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTATCTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATATCG  >  NC_000913/3953769‑3954255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑