Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,763,456	G→A	L29L (CTG→TTG)	abpB ←	putative helicase YfjK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,763,456	0	G	A	100.0%	83.9 / NA	25	L29L (CTG→TTG)	abpB	putative helicase YfjK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/8);  total (17/8)

GGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCC  >  NC_000913/2763222‑2763702                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       ggAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAAc                                                                                                                                                                                                                                        <  2:85504/251‑1 (MQ=255)                ggAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGGGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGAt                                                                                                                                                                                                                         <  2:239184/251‑1 (MQ=255)                  aaaaCATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATga                                                                                                                                                                                                                       <  2:65589/251‑1 (MQ=255)                       aTAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGt                                                                                                                                                                                                                  >  1:47473/1‑251 (MQ=255)                                  agaATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGc                                                                                                                                                                                                       >  1:165493/1‑251 (MQ=255)                                       ggCGTCCGGAATAGCTCAAGCACCCTCAGGTCATCAGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGCGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCt                                                                                                                                                                                                  >  2:181857/1‑251 (MQ=255)                                          gTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATg                                                                                                                                                                                               <  1:5521/251‑1 (MQ=255)                                                                           ggTGTCAGGCTGGTGAACTCCTTTTTGAGATGCGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGTCTGTTCATAGATCTCTGTCATACGAcc                                                                                                                                                              <  2:212696/251‑1 (MQ=255)                                                                                cAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACctctct                                                                                                                                                         >  1:238865/1‑251 (MQ=255)                                                                                                             ggAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAAcc                                                                                                                                                                                                                                              >  2:221059/1‑137 (MQ=255)                                                                                                             ggAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAAcc                                                                                                                                                                                                                                              <  1:221059/137‑1 (MQ=255)                                                                                                              gAACAAACCTGATTTTCTCACCATATTTCTCCAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGATCTCTCTCACCTTCTCATCAACTAATCGGGTTAACCg                                                                                                                           >  1:234326/1‑251 (MQ=255)                                                                                                                            ttCTCACCATATTGTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCggg                                                                                                              <  1:150114/250‑1 (MQ=255)                                                                                                                               tcACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTg                                                                                                          >  1:217123/1‑251 (MQ=255)                                                                                                                                         tttCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGa                                                                                                 >  1:243274/1‑250 (MQ=255)                                                                                                                                                aTTGTGGTGTGCTCGGAAAACCGATCGCGCGAATCGAGAGATCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTgg                                                                                         >  2:249975/1‑251 (MQ=255)                                                                                                                                                                   aCCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCCAACg                                                                      >  2:7388/1‑251 (MQ=255)                                                                                                                                                                             cgAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTc                                                            >  2:244393/1‑251 (MQ=255)                                                                                                                                                                                     gagCTCGCAAGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGt                                                    >  2:138219/1‑251 (MQ=255)                                                                                                                                                                                          cGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGcc                                               >  1:188273/1‑251 (MQ=255)                                                                                                                                                                                          cGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCATGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCACACGGCGAATCCTCGATGAGGTCAGcc                                               >  1:12158/1‑251 (MQ=255)                                                                                                                                                                                                     gtcccgtcccAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCAGCGAATTTTTc                                    >  1:258175/1‑251 (MQ=255)                                                                                                                                                                                                            cccAAGTCATACGAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAg                              <  1:181857/250‑1 (MQ=255)                                                                                                                                                                                                                        gAAACAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGtttct                 >  1:71377/1‑251 (MQ=255)                                                                                                                                                                                                                            cAGGGTTAGACAACAACTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTttctttct             >  1:116374/1‑251 (MQ=255)                                                                                                                                                                                                                                      caacaaCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCCGGCAATGTTTCACCTGTTCATAGATCTCTGTCATACGACCTCTATAACCTTCTCATCAACTAATTGGGTTAACCGATCAAGACTCGTGGCTGGATAAATGAATACGTGGATGCAAAGCTTCGCAAACGGCGAATCCTCGCTGAGGTCAGCCGCGAATTTTTCGAACAGCAACGCTGTTTCTTTCTACAGGTCATc   >  1:260513/1‑251 (MQ=255)                                                                                                                                                                                                                                       aacaacTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATcc  <  2:116374/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       GGAACTGCATTGAGTGGAAAACATAACCATCCGAGAATGGCGTCCGGTATAGCTCAAGCACCCTCAGGTCATCCGGTGTCAGGCTGGTGAACTCCTTTTTGAGATACGGGAACAAACCTGATTTTCTCACCATATTTTTCAAAATTGTGGTGTGCTCGGAAAACCGCTCGCGCGAATCGAGAGCTCGCACGATCAAGTCCCGTCCCAAGTCATACGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCC  >  NC_000913/2763222‑2763702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑