Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,484,496	G→A	A3T (GCC→ACC)	prkB →	putative phosphoribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,484,496	0	G	A	95.7%	68.9 / ‑6.7	23	A3T (GCC→ACC)	prkB	putative phosphoribulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (12/10);  total (12/11)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

TGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATT  >  NC_000913/3484279‑3484741                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      tgtTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATcc                                                                                                                                                                                                                       >  1:80940/1‑250 (MQ=255)          aaGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCgg                                                                                                                                                                                                             >  1:18717/1‑251 (MQ=255)            ggCACCGATTATGGAGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGAATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCgggg                                                                                                                                                                                                           >  2:230884/1‑251 (MQ=255)                       tGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGaccaccaccac                                                                                                                                                                                                >  1:206862/1‑251 (MQ=255)                                 gAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTcgcg                                                                                                                                                                                      >  1:169379/1‑251 (MQ=255)                                     gTACACTTGAACATAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTc                                                                                                                                                                                  <  2:80940/251‑1 (MQ=255)                                       acacTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGGAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCg                                                                                                                                                                                 <  2:52634/250‑1 (MQ=255)                                                              cGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACTGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCTAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGTACCACCCCCCCCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCt                                                                                                                                                         <  1:24921/251‑1 (MQ=255)                                                                  aaaCGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGGCGGCGGACGAGATAATAATGAGAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCtgcat                                                                                                                                                     <  2:125998/251‑1 (MQ=255)                                                                                               gCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTc                                                                                                                         >  1:128226/1‑250 (MQ=255)                                                                                                                     gAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAATCATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATg                                                                                                  <  2:274586/251‑1 (MQ=255)                                                                                                                     gAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATg                                                                                                  >  1:148602/1‑251 (MQ=255)                                                                                                                      aaaCGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATgg                                                                                                 <  1:136089/251‑1 (MQ=255)                                                                                                                        aCGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGAc                                                                                               <  1:143303/251‑1 (MQ=255)                                                                                                                                 attatGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATc                                                                                      >  1:227012/1‑251 (MQ=255)                                                                                                                                                    tgcgcgAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCTGAAATGGACATGGCGATCCGCAAAGCGCGCGACGcc                                                                    <  2:128226/248‑1 (MQ=255)                                                                                                                                                      cgcgAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACAACCGCCCGGAAATGGACATGGCGATCCGAAAAGCGCGCGCCACCggg                                                                 >  2:233237/1‑251 (MQ=255)                                                                                                                                                                       cGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACtt                                                >  2:112409/1‑251 (MQ=255)                                                                                                                                                                             cggACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGccc                                          >  1:13457/1‑251 (MQ=255)                                                                                                                                                                                           atGATAGCTAAGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTCAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGTAGGCGACAGTTTTCACCGTTACCCCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACt                            >  1:183168/1‑251 (MQ=255)                                                                                                                                                                                                     aGTCATGGAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCctgct                  <  2:48793/251‑1 (MQ=255)                                                                                                                                                                                                           ggAGTTACTATGTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACa            <  1:226620/251‑1 (MQ=255)                                                                                                                                                                                                                      gTCTACCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCAtt  <  2:227012/250‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      TGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATT  >  NC_000913/3484279‑3484741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑