Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,555,270	C→T	T153T (ACG→ACA)	eutK ←	putative structural protein, ethanolamine utilization microcompartment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,555,270	0	C	T	100.0%	93.2 / NA	30	T153T (ACG→ACA)	eutK	putative structural protein, ethanolamine utilization microcompartment
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/8);  total (22/8)

CTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTGCTTCGGCTGGCGGTTAAAGCCAGTAACCAGCCACTGGGTGTCATCGTCTGGCCGACCGA  >  NC_000913/2555030‑2555502                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         ctgcCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGGAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATTTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGc                                                                                                                                                                                                                                  >  1:194138/1‑249 (MQ=255)          ggTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCAACTTCGACCTTCGGCGTGAGCTTAAGATTTGCGGGTAAGGGTTCATGATAAAGAGGGTGCAAATTGGATGTACGGGTCTTTTTCATGATGTTAATGCCGGGAGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACAGCGTTTACGTAATGTCCCGGCAGAAAAGAGCt                                                                                                                                                                                                                       >  1:165215/1‑251 (MQ=255)             aaTCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGtt                                                                                                                                                                                                                    >  2:109664/1‑251 (MQ=255)                    aaaGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAAAATTATCCACTTCGACAATCGGCGTGAGCTTCAGGTTTTAGGGTAAGGGATCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGGTGTAGGACACCGGGCACCTCCGACAGGTTAATGGGGCTTGAGACGAAAACGACTACTGCGTTTACGTAATGTCCCGGAAGCAAAGAGCTGTTCGAgcgc                                                                                                                                                                                                             >  2:126465/1‑251 (MQ=255)                            ttCATAGACATCCGTTGTCTGTCGTTGATGAAAATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTgg                                                                                                                                                                                                     >  1:86347/1‑251 (MQ=255)                                    cATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTcc                                                                                                                                                                                             >  2:44944/1‑251 (MQ=255)                                                     tgatgaACATTATCCACTTCGACCTTCGGAGTGGGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTACTGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTCATGGGGCTTGAGACGATCACGACTACTGCGTTTACGTAATGTCCCGGCAGAAACGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTg                                                                                                                                                                            >  1:266430/1‑251 (MQ=255)                                                                         gACCTTCGGCGTGAGCTTTAGGTTTTCTGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCATAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCg                                                                                                                                                        <  2:263370/251‑1 (MQ=255)                                                                                          tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTa                                                                                                                                         <  1:43555/249‑1 (MQ=255)                                                                                                      gTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAgc                                                                                                                           >  1:159264/1‑251 (MQ=255)                                                                                                                   aTAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATACCGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGt                                                                                                              >  2:243467/1‑251 (MQ=255)                                                                                                                               cAAATTGGCTGTACGGGGCTTTTGCATGAGGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGTCAGGTAAATGGGGCTTGAGACGATAACGTCGACTGCGGTTACGTAATGTCCCGGCAGAAAAGAGCGGTTCGAGCGCATTTCTGGCTGTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACGTCGCCTGCCGCCATTCCTAGAGGTACTGAGGTTAACATCGCCAACAACTCTTCATCAGagaccg                                                                                                  <  2:266430/251‑6 (MQ=255)                                                                                                                                         gTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCTGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAAc                                                                                        >  1:60284/1‑251 (MQ=255)                                                                                                                                          tACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCAGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGAGTTTACGTAATGTCCCGGCAGAAAAGAGATGTTCGAGCGCATTTCTGGCTTTTTCAAGCGGCCAGACAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCACCAACTCGTCAGCAGATTCagcgtctctaact                                                                                       >  2:184736/1‑242 (MQ=255)                                                                                                                                                           atgTTAATGCCGGGTGTTGTAGGACACCCGGCCCCTCCGACAGGTTAATGGGGCTTGAGACGATAACTACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTAAGCAGATTCCGCGACGATAACTGGCGCGTCGGTTTCCCt                                                                      <  2:216639/251‑1 (MQ=255)                                                                                                                                                                    ccGGGTTTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGCCTACTGCGTTTACGTAATGTCCCGGTAGAAAAGAGCTGTTCCAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCACCAACAACTCGTCAGCATATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTg                                                             >  1:157925/1‑251 (MQ=255)                                                                                                                                                                    ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTg                                                             >  2:58211/1‑251 (MQ=255)                                                                                                                                                                    ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTg                                                             >  1:10848/1‑251 (MQ=255)                                                                                                                                                                    acGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGACTTGAGACAATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGACAAAGGGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACAGATGTTAAAAGCGCCAACAACTCGTAAGCAGATTCCGCGACGATAACTGGAGCGTCGGGTTCCCTTACGGGTTg                                                             >  1:10851/2‑251 (MQ=255)                                                                                                                                                                                               ccGACAGGTTAATGGGGCTTGAAACGATAACGACTACTGCGGTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTATGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCACCAACTCGGCAGCAGCTTCCGCGACGATAACTGACGCGGCGGGGTCCCTTACGGGTTGCTTCGGATGGCGGTTTAAgacaacaac                                  >  2:142186/1‑243 (MQ=255)                                                                                                                                                                                                         aaTGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTGCTTCGGCTGGCGGTTAAAGCCAGTAACCAGCCACTgg                        <  2:58482/251‑1 (MQ=255)                                                                                                                                                                                                            ggggCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTGCTTCGGCTGGCGGTTAAAGCCAGTAACCAGCCACTGGgtg                     <  1:46201/251‑1 (MQ=255)                                                                                                                                                                                                                  tGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTGCTTCGGCTGGCGGTTAAAGCCAGTAACCAGCCACTGGGTGTCAt                 <  1:94681/249‑1 (MQ=255)                                                                                                                                                                                                                    agaCGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTGCTTCGGCTGGCGGTTAAAGCCAGTAACCAGCCACTGGGTGTCATCGTc             >  1:91267/1‑251 (MQ=255)                                                                                                                                                                                                                      aCGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTCCCAGCAGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTGCTTCGGCTGGCGGTTAAATCCAGTAACCAGCCACTGGGTGACATCGTCTg           >  1:131370/1‑251 (MQ=255)                                                                                                                                                                                                                        gATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTGCTTCGGCTGGCGGTTAAAGCCAGTAACCCGCCACTGGGTGTCATCGTCTGGc         >  2:165680/1‑251 (MQ=255)                                                                                                                                                                                                                           aaCGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTGCTTCGGCTGGCGGTTAAAGCCAGTAACCAGCCACTGGGTGTCATCGTCTGGccg       <  1:58211/250‑1 (MQ=255)                                                                                                                                                                                                                              gACTACTGCGTTTACGTAATGTCCCGGCAGAACAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGACGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTGCTTCGGCTGGCGGTTCAAGCCAGTAAACACCCACTTGGTGTCATCGTATGGccgtccg   >  1:242006/1‑251 (MQ=255)                                                                                                                                                                                                                               actactGCGTTTACGTAATGTCCCGGCCGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTGCTTCGGCTGGCGGTTAAAGCCAGTAACCAGCCACTGGGTGTCATAGTCTGGccgaccgg  >  1:248434/1‑250 (MQ=255)                                                                                                                                                                                                                               actactGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTGCTTCGGCTGGCGGTTAAAGCCAGTAACCAGCCACTGGGTGTCATCGTCTGGccgaccg   >  1:156414/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         CTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTCATTCCCTGACGTACTGATGTTAACAGCGCCAACAACTCGTCAGCAGATTCCGCGACGATAACTGGCGCGTCGGGTTCCCTTACGGGTTGCTTCGGCTGGCGGTTAAAGCCAGTAACCAGCCACTGGGTGTCATCGTCTGGCCGACCGA  >  NC_000913/2555030‑2555502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑