Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,453,265	C→T	intergenic (‑929/+1818)	yfcS ← / ← yfcV	putative fimbrial chaperone YfcS/putative fimbrial protein YfcV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,453,265	0	C	T	100.0%	95.7 / NA	28	intergenic (‑929/+1818)	yfcS/yfcV	putative fimbrial chaperone YfcS/putative fimbrial protein YfcV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/16);  total (12/16)

GCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCCCAGTAGGTATGGCGGGTATAGTTGAGATAAACCGAAACACCCGCATCGCGGAAGTTCTGGTTATAAGTGGCGGTGTACATCTCT  >  NC_000913/2453026‑2453481                                                                                                                                                                                                                                                |                                                                                                                                                                                                                         gCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCtt                                                                                                                                                                                                               >  2:67919/1‑251 (MQ=255)   agagGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTAt                                                                                                                                                                                                             <  1:260605/251‑1 (MQ=255)     gggTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATcc                                                                                                                                                                                                           <  2:24339/250‑1 (MQ=255)       gtgtATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCCTACTGAGCGAAATTTACATGCCCTTATCCGc                                                                                                                                                                                                         >  2:187471/1‑251 (MQ=255)        tgtATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGcc                                                                                                                                                                                                        >  1:180470/1‑251 (MQ=255)         gtATTGCCCTTCATGGTATTTCGCACTTAGGTCAACCTGCTCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGccc                                                                                                                                                                                                       <  2:181640/251‑1 (MQ=255)            ttGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGt                                                                                                                                                                                                    >  2:75296/1‑251 (MQ=255)                   tCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCAt                                                                                                                                                                                              >  2:49882/1‑250 (MQ=255)                          aGTTCGCACTGAGTTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTa                                                                                                                                                                                       <  2:29722/250‑1 (MQ=255)                                      ggTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGCGAGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCNGTc                                                                                                                                                                          >  2:255474/1‑251 (MQ=255)                                                        aaCCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATAtt                                                                                                                                                         >  1:270824/1‑250 (MQ=255)                                                        aaCCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTa                                                                                                                                                        <  1:75296/251‑1 (MQ=255)                                                                     gtagtaGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGccc                                                                                                                                            <  1:73616/250‑1 (MQ=255)                                                                                                   aCTGGTGCCGATGTTCAACTGATAGTGAGGCGCTTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGAtgttgt                                                                                                             <  2:270824/251‑1 (MQ=255)                                                                                                      ggTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGt                                                                                                          >  2:202012/1‑251 (MQ=255)                                                                                                       gTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGtt                                                                                                         <  1:49882/251‑1 (MQ=255)                                                                                                        tGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGttt                                                                                                        >  2:232968/1‑251 (MQ=255)                                                                                                          ccGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGt                                                                                                      >  1:171595/1‑251 (MQ=255)                                                                                                           cGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTc                                                                                                     <  1:67919/251‑1 (MQ=255)                                                                                                                                  gcgTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCCCAGTAGGt                                                                              <  2:284775/251‑1 (MQ=255)                                                                                                                                    gTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACCGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCCCAGTAGGTAt                                                                            >  2:33809/1‑251 (MQ=255)                                                                                                                                               ggCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATAATGTTGTAGTTTGTCTGCTCCTCACGATCCCAGTAGGTATGGCGGGTATAg                                                                 <  2:227862/251‑1 (MQ=255)                                                                                                                                                   aaaaTAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCt                                                                                                    <  1:144531/212‑1 (MQ=255)                                                                                                                                                   aaaaTAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGGGGGAGAGCATGATGTTGTAGTTTGTCt                                                                                                    >  2:144531/1‑212 (MQ=255)                                                                                                                                                    aaaTAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCCCAGTAGGTATGGCGGGTATAGTTgag                                                            <  1:168538/251‑1 (MQ=255)                                                                                                                                                                        ttccgtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCCCAGTAGGTATGGCGGGTATAGTTGAGATAAACCGAAACACCCGc                                          <  2:238694/248‑1 (MQ=255)                                                                                                                                                                                                               aCTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCCCAGTAGGTATGGCGGGTATAGTTGAGATAAACCGAAACACCCGCATCGCGGAAGTTCTGGTTATAAGTGGCGGTGTACATctct  <  1:108794/250‑1 (MQ=255)                                                                                                                                                                                                                                     cTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCCCAGTAGGTATGGCGGGTATAGTTgag                                                            <  1:132947/170‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                         GCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCCCAGTAGGTATGGCGGGTATAGTTGAGATAAACCGAAACACCCGCATCGCGGAAGTTCTGGTTATAAGTGGCGGTGTACATCTCT  >  NC_000913/2453026‑2453481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑