| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 323,418 | G→A | A361T (GCC→ACC) | ykgF → | putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 323,418 | 0 | G | A | 100.0% | 34.9 / NA | 13 | A361T (GCC→ACC) | ykgF | putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (7/6); total (7/6) | |||||||||||
GAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGG‑TGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCG > NC_000913/323199‑323661 | gagTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATTGCTCTATTTTTCCAGGGCCCATTGGTGCGGGTG‑TTTCTCCGCTACTTTGCGGCTATAAAGATTTTTAAGATTTACCCTACACCTGCTCTTTATTCACAGCTTTTGACAACgt > 1:5493/1‑251 (MQ=255) aTTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGG‑TGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACACCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTa < 1:22650/250‑1 (MQ=255) ggCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGG‑TGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACACCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCaaaa < 1:216475/251‑1 (MQ=255) ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGG‑TGATTTCTCCGCTTCTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACACCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCAtcgtcg < 2:10816/215‑1 (MQ=255) aCTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATACCGGGCCAATTGGTGCGG‑GGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCCACACCTGCTCTTTTTTCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCCGTCAAAACTGATTTTTCGTCATTGTCGGGTGATGGGTGAAAAAAGGATCAACGCAAAAGCAAGGCAACGGGCGATaa > 1:278100/1‑250 (MQ=255) ggATATGGCTCTATTTATCCAGGGCCAATTGGTGCGG‑TGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACACCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGAtt > 2:141769/1‑251 (MQ=255) atatGGCTCTATTTATCCAGGGCCAATTGGTGCGG‑TGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACACCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATtgt < 2:79461/251‑1 (MQ=255) tctATTTATCCAGGGCCAATTGGTGCGG‑TGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACACCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGt > 1:204256/1‑251 (MQ=255) acccAGGTCCAATGGGGGCGG‑TGATTTCTCCGCTACTTGGCGGCTATAAAGATGTTAAAGATTTACCCTACACCTGCTCTTTATACACAGCTTGTGACAACTTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCGGAAAAAGTGATCGCCGCAAAAGCAGAGCAACTGGCTATAAAAATGTTCGCTTATGCAAATAGTCATTTAGGATTGTAGAAAGTccgt < 2:171373/246‑4 (MQ=255) ttGGCGGCTATAAAGATTTTAAAGATTTACCCTACACCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAAt > 2:186875/1‑251 (MQ=255) aaagattttaaagatttACCCTACACCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAac > 2:269278/1‑251 (MQ=255) ttaaagatttACCCTACACCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACt < 1:62503/250‑1 (MQ=255) aCCCTACACCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGgcg > 1:176663/1‑251 (MQ=255) | GAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGG‑TGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCG > NC_000913/323199‑323661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |