Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,032,423	G→A	V219V (GTC→GTT)	yedJ ←	putative HD superfamily phosphohydrolase YedJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,032,423	0	G	A	100.0%	89.4 / NA	27	V219V (GTC→GTT)	yedJ	putative HD superfamily phosphohydrolase YedJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/12);  total (15/12)

ATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAAT  >  NC_000913/2032189‑2032642                                                                                                                                                                                                                                           |                                                                                                                                                                                                                            atactcACCTACACCATTAAGCCGCGCCACCAACGCTCTAACATCATCACTTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGACTCGGTTACTGATATCTTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGCCAGGTTAACATAATTAAAGCCATTTAGGGCTACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTccc                                                                                                                                                                                                             >  2:182901/4‑251 (MQ=255)   tctcACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTcccc                                                                                                                                                                                                            <  1:115418/250‑1 (MQ=255)                    aGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCCGAATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATAAAAACGCATCTATCCCCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAg                                                                                                                                                                                          <  2:15912/251‑1 (MQ=255)                         caccaccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATGGCCTGGGCGGCATTTCCGTGGCTGTATGAATCGTTTACTTATATATTTTCCTGCATAGTTTTCACCGCCCATCGGAGATGGCCGACAGTTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCTCCTTGTGATCAACACCTCCATTCTCCCCCGCCAGTTCGGCACTGATCTTCg                                                                                                                                                                                     <  2:219294/249‑1 (MQ=255)                              ccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCAt                                                                                                                                                                                 >  1:82208/1‑250 (MQ=255)                              ccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCAt                                                                                                                                                                                 >  1:62469/1‑250 (MQ=255)                                    ttttAACATCATAATTTTGCAGCAGTTTCTCACGCATTGCCTGTGGGGCATTCCCGGTGCTGTATGAATCTGTTACTAATATGTTTTCCTGCATAGATTTCCCCGGCCATCTGGGATGGCCGACAGGTTAACATAATTACAGCCATTTGGTGCCGCGGGCTCAGCCAGCGGATGAAACCGCATCTATCCCCTTTGGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTc                                                                                                                                                                           <  1:23156/250‑1 (MQ=255)                                           atcatAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTaaa                                                                                                                                                                    >  1:45825/1‑250 (MQ=255)                                             ataaaaTGTGCAGCAGTTTATCCAGCATTTCCTGTGCGGCATTCCCGGGTCTGTATGAATCGGTTACTGAAATAATTTCCTGCATAAATTTCACCGGCACTCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCGGCCAGCGGATGAAACCGCATCTATCCCCTTGTGATCACCACCTTCATGCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTaaaaaa                                                                                                                                                                 <  2:224139/248‑1 (MQ=255)                                                        agcagTTTCTCCAGCATTGCCGGGGCGGCATTCCCGGTGCTGTATGATGCGGTTACTGATATATTTTCCTGCAGAGATTTCACCGGCCTTCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCTCTCAGCCAGGGTATGAAAACGCATCTATCCCTTTTTGATCAACCCCTTCATTCCCCCCCGCCAGTGCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTtgtg                                                                                                                                                      <  1:44501/251‑1 (MQ=255)                                                          ccgTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGACTCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGGTGtgct                                                                                                                                                    >  1:228217/3‑251 (MQ=255)                                                          cagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGtgct                                                                                                                                                    >  1:228209/1‑251 (MQ=255)                                                           agTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGtgctg                                                                                                                                                   <  1:70483/251‑1 (MQ=255)                                                               tctcCTGCATTGCCTGTGCGGCATTAGCGTTGCTGTATGAATGGGTTACTGATATATTTTCCTGCATCGATTTCACCGGCTTTATGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGGAACGCGCTCAGCCAGCGGATGAAAACGCATATATCACATTGTGATCAACACCTTCATTCTCCCTCGCCAGGTCGGCACTACGCTTCGACATAAACTAGACTAACAAATACGGGTTGGGCAGCGcc                                                                                                                                               >  2:39721/1‑251 (MQ=255)                                                                    aGCATTGCTTGTGCGGAATTCCCGGTGCGGTAGGAATCGGTTACTGTAATATTTTCCTGCATAGATTTCCCCGGCCATCTGAGATGGCCGACAGGTTAACAAAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAgctg                                                                                                                                          <  2:101707/251‑1 (MQ=255)                                                                        gttccgttgCGGCATCCCCGGTGCGGTATGAATCGGTGTCTGAGATAGTTTCCGGTATATATTGCACCGGCCTTCGGAGAGGGACGACATGTTAGCATAATTACAGCTGTTTAGGGCCACGCGCTCAGCCAGCGGGTAAAAACTCATCTATCACCTTGTGATCAACCCCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCATCTGCtt                                                                                                                                       <  1:262823/243‑1 (MQ=255)                                                                                gCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGCGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCttcc                                                                                                                              >  2:34567/1‑249 (MQ=255)                                                                                             tGCTGTCTGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCGGAGATGGCCGATAGGTTAACATAATTACAGCCCTTTAGAGCCACGCGATCAGCCAGCGGATGAAAACGCATCTATCACCTTGCGATCAACACCTTAATGCTCCCCCGCCAGTTCGGCACTGAGTTTCGCCATCAACTCGACTAAAAAATGCGCGTTGTGCTGCGACAGCTGCTTGCCTCTTGCGGTTCGCATGGtt                                                                                                                 >  1:205601/1‑251 (MQ=255)                                                                                                           ggtACTGATATATTTTCCAGCATAGATTGCACCGGCCATGTGAGAGGGCCGACGGGTTAACATAATTACAGCCATTTAGGTGCACGCGATCAGCCAGCGGATAAAACCGTATCTATCACCTGGTGATCAACACCTACATTCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCGTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAg                                                                                                   <  2:2617/249‑1 (MQ=255)                                                                                                            ttACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGc                                                                                                  >  1:120810/1‑251 (MQ=255)                                                                                                                                                    gagaTGGCCGACAGTTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCCCCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAg                                                                           <  2:32129/234‑1 (MQ=255)                                                                                                                                                    gagaTGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAg                                                                           >  1:32129/1‑234 (MQ=255)                                                                                                                                                                                          tgCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATc                     >  2:266852/2‑250 (MQ=255)                                                                                                                                                                                                  gcTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAgcgc            >  1:283514/1‑251 (MQ=255)                                                                                                                                                                                                    tCAGCCAGCGGCTGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATgc                                                                                                                                                              >  2:24591/1‑103 (MQ=255)                                                                                                                                                                                                    tCAGCCAGCGGATGAAAACGCATCTATCTCCTTGTGATCAACCCCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATgc                                                                                                                                                              <  1:24591/103‑1 (MQ=255)                                                                                                                                                                                                            cGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAAt  >  1:183829/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                            ATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAAT  >  NC_000913/2032189‑2032642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑