Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,222,234 G→A G257S (GGC→AGC)  ebgR → DNA‑binding transcriptional repressor EbgR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,222,2340GA94.4% 45.5 / ‑6.2 18G257S (GGC→AGC) ebgRDNA‑binding transcriptional repressor EbgR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (10/7);  minor base T (0/1);  total (10/8)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTGGTGTATGAAAAAGCCCGCGATGGTCGCGCGCTGCCGCTGTTAGTCTTCGTTCCCAGCAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTT  >  NC_000913/3222024‑3222478
                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    
ggCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAAGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTaa                                                                                                                                                                                                              <  2:75667/251‑1 (MQ=255)
   aaGGCGGATATTCGTGAGGTCGACTTTACGGAATATGGCCGACTGAAACCAGTGGAACGCGAAGAGGATATCTGGCGCGGCGGATTTTCCAGTTCGTCCGGTTATGAACTGGCAAAACAAAAGATGGAGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTAATGAGGGCAATTAATCAACGAAGCCTGAACATCCCACAGGAAATTTCGCTTATCAGCGTTAACg                                                                                                                                                                                                            >  2:112931/1‑250 (MQ=255)
                               cGGAATATGGCCGACTGAAACAAGTGGAACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAAGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTTCCTTTccg                                                                                                                                                                               >  1:250962/1‑251 (MQ=255)
                                 gAATATGGCTGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTGTTTCCAGTTCGTCGGGTTATTAACTGGCAAAACAAATGCTTGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGATGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTGAACGATATCCCCTCCGCGCGATTTACCTTTccg                                                                                                                                                                               <  1:87276/249‑1 (MQ=255)
                                  aaTATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTTTGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTTCGGGCAATTCATGAACGAAGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccgccc                                                                                                                                                                            >  1:244885/1‑250 (MQ=255)
                                               gAAACACGTGGTACGCGAAGCGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGGTATGAACTGGCAAAAAAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAAGCCTGAACATCCCACAGGGTATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTTCGCCGCCCTCCCCCGTgc                                                                                                                                                               >  1:87356/1‑251 (MQ=255)
                                                  aCAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAAGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                                                                                                                                                                                         <  1:63279/222‑1 (MQ=255)
                                                                            ggCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAAGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAg                                                                                                                                  <  2:277051/251‑1 (MQ=255)
                                                                                                      ggTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTAGCGGCGTACTGCGGGCAATTCATGAACGAAGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTGGTGTATGAAAAAGc                                                                                                        <  1:38336/251‑1 (MQ=255)
                                                                                                          aTGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAAGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCGTTCCGAGATGATGGGAAGTAAGGGCGTAACCCTGGTGAATGAAAAAGccagc                                                                                                    >  1:280914/1‑248 (MQ=255)
                                                                                                             aaCTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAAGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTGGTGTATGAAAAAGCCAGCGAt                                                                                                 >  2:54135/1‑251 (MQ=255)
                                                                                                                     aaaCAAATGCTGGCGCGGGAAGACTAACCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTAGCGGCGTACTGCGGGCAATTCATGAACGAAGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTGGTGTATGAAAAAGCCCGCGATGGTcgcgc                                                                                         <  1:202346/251‑1 (MQ=255)
                                                                                                                       aCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAAGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTGGCGTATGAAAAAGCCCGCGATGGTCgcgcgc                                                                                       >  1:264824/1‑251 (MQ=255)
                                                                                                                                                          tgtttgttGCTTCAGATTCCATTGCTATCGGCGTAATGCGGGCAGTTCATGAACGAAGCCTGAACATACCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCAGCTCTCCACCGTGCGCATCCATTCAGAGATGATGGGAAGTCAGGGCGTTCACCTGGTGTATGAAAAAGCACGCGATGGTCGCGCGCTGCCGCTGTTAGTCTTCGTTCCCCGCAAATTnaa                                                     >  2:262153/1‑250 (MQ=255)
                                                                                                                                                                                       ggCGTACTGCGCGCAATTCATGAACGAAGCCTGAACATCCCACAGGATATTTCGCTTCTCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTGGTGTATGAAAAAGCCCGCGATGGTCGCGCGCTGCCGCTGTTAGTCTTCGTTCCCAGCAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCtt                       >  2:110461/1‑251 (MQ=255)
                                                                                                                                                                                        gCGTACTGCGGGCAATTCATGAACGAAGCCTGAACATCCCACAGGATATTTCGCATATCAGCGTTAACGATATCCCCACCGCGCGCTTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGCTGGGAAGTCAGGGCGTTAACCTGGTGTATGAAAAAGCCAGCGATGGTCGCGCGCTGCCGCTGTTAGTCTTCGTTCCCAGCAAATTAAAACTGCGCGGCACAACCCGTTAAATACCCtc                       >  1:259293/1‑249 (MQ=255)
                                                                                                                                                                                                            gAACGAAGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCGCCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGGTAACCTGGTGTATGAAAAAGCCCGCGATGGTCGCGCGCTGCCGCTGGTAGTCTTCGTTCCCAACAAATTAAAACGGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGt   <  1:262153/250‑1 (MQ=255)
                                                                                                                                                                                                            gAACGAAGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTGGTGTATGAAAAAGCCCGCGATGGTCGCGCGCTGCCGCTGTTAGTCTTCGTTCCCAGCAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGtt  <  1:112931/251‑1 (MQ=255)
                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    
GGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTGGTGTATGAAAAAGCCCGCGATGGTCGCGCGCTGCCGCTGTTAGTCTTCGTTCCCAGCAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTT  >  NC_000913/3222024‑3222478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑