Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,623,009	C→T	P414L (CCG→CTG)	yjjJ →	toxin YjjJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,623,009	0	C	T	100.0%	92.1 / NA	29	P414L (CCG→CTG)	yjjJ	toxin YjjJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/17);  total (12/17)

TTTGGGCGACTTATCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCC  >  NC_000913/4622768‑4623245                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             tttGGGCGACTTATCGCCAACAGCGATATGCACGCAGGTAATTTATCTTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCa                                                                                                                                                                                                                                      <  1:10969/250‑1 (MQ=255)     ggCGATTTATCCCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAg                                                                                                                                                                                                                                 <  1:126600/251‑1 (MQ=255)             aTCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGACCCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTc                                                                                                                                                                                                                          <  2:187303/250‑1 (MQ=255)             aTCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCg                                                                                                                                                                                                                         <  1:198805/251‑1 (MQ=255)                    ccagcttttttccctgaGGTTATTTATCTTTTTATTTATCTGTCCCGCCACTTGCGCGGTCGCCCGTCTTCGACATGCTTACGATGGGTTATGTACCAAACTGGTCTTGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTGTATCTTAACGTCAGTAAACGCGCTTGGTTAACGTCGATCCCGCTGGCGCATCAGTTCTGGCACACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACAtt                                                                                                                                                                                                                  <  2:97391/235‑1 (MQ=255)                    aCAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACAtt                                                                                                                                                                                                                  >  2:86301/1‑251 (MQ=255)                     aaGCGATATGCCCGCAGGTAATTTATCGTTTTAGTTATCTAAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAGACGGTCGCCAGAGATCTGCGTATCAGCTAGGCGTTTCGCCACATTg                                                                                                                                                                                                                 <  1:165964/250‑1 (MQ=255)                      aGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTgc                                                                                                                                                                                                                >  1:184621/1‑251 (MQ=255)                          atatGCACGCAGGTAATTTATCTTTTGATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGGGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCaa                                                                                                                                                                                                            <  1:134400/251‑1 (MQ=255)                           tatGCACGCAGGTATTTTATCGTTTTATTTATCTGAGCCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTTATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGTCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGTCGTTTCGCCTCATTGCGCaa                                                                                                                                                                                                            <  2:264553/250‑1 (MQ=255)                                     ggTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCgg                                                                                                                                                                                                  >  1:277710/1‑250 (MQ=255)                                     ggTAATTTATCGATTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCgg                                                                                                                                                                                                  >  1:32136/1‑250 (MQ=255)                                                     atttatCTGAACCGCCATGTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATc                                                                                                                                                                                 <  1:132205/251‑1 (MQ=255)                                                                 cGCCATTTGCGCTGACGTCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGTTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGtt                                                                                                                                                                     <  2:125778/251‑1 (MQ=255)                                                                       ttgcgcgtaCGCCCGTCTACGACATGCTGCCGATGGTCTATGCCCCAAACAGCGCTGGAATGGTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCg                                                                                                                                                                <  2:65122/242‑1 (MQ=255)                                                                                   ccGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTa                                                                                                                                                    >  1:260048/1‑250 (MQ=255)                                                                                         aCGACATGCTGCCGATGGTCTATGCACGAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCt                                                                                                                                             <  2:68354/251‑1 (MQ=255)                                                                                           gACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCtt                                                                                                                                            >  1:279805/1‑250 (MQ=255)                                                                                                   gcCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACAGccccc                                                                                                                                   <  1:68622/251‑1 (MQ=255)                                                                                                                                              gctgcCATTGAGGTGAAGTTTGATCTTAACGTCAGCAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTAAAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCg                                                                                        >  2:53717/1‑251 (MQ=255)                                                                                                                                                       gAGGTGAAGTTGGATGTTAAAGACAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGAAAAAAATCCGGCAAATCGAAGAGAAAGTTTCCCGCATGGTCGGGTAACTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTcaa                                                                               <  2:147290/251‑1 (MQ=255)                                                                                                                                                                           cGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAgaga                                                                                                                                                                          <  1:11847/140‑1 (MQ=255)                                                                                                                                                                           cGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAgaga                                                                                                                                                                          >  2:11847/1‑140 (MQ=255)                                                                                                                                                                                                     cGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCTAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACAGCCCCCGCCCTCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCACCCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAg                                 <  1:242857/251‑1 (MQ=255)                                                                                                                                                                                                      gATCCCGCTGGCGCAGAAGTTTTGGCAAACGGACGCCAGAGATCTGAGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGACATGCCGGAAAAATTACGGAATGTCGAAGAGAAAGTAGCAAGCATGGGCGGGGAAATAAATAACAGCCCCAGCCAAGCATGCCGATAACTCCCGTAGCACGATGTCCTGTTCAGGGAAGTTACCCACCACCGCTTCGCACTCCTGTTCAACCATATCTGCGCGGTACAgg                                >  2:183161/1‑251 (MQ=255)                                                                                                                                                                                                        tCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGc                               >  1:63390/1‑250 (MQ=255)                                                                                                                                                                                                               ggCGCAGCAGTTCTGGCAAACTGTCGCCAGAGATCTGCGTATCAGCGAGGCTTTTCGCCTCATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGATAAATTTGCCCGCATGGGCGGGTAACTCCCTTTCAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCATCCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTCCAGGCAGCCtt                         <  1:119157/249‑1 (MQ=255)                                                                                                                                                                                                                       agTTCTGGCAACCGGTCGCCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGAGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCTTcc               >  2:154807/1‑251 (MQ=255)                                                                                                                                                                                                                                    ggTCGGCAGAGATCTGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGAGGGTAACTACCTTACAGCCCCCGCCATACATGACGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGGCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTAGTCCGGCGAGGGCTTcc  >  2:18068/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             TTTGGGCGACTTATCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCC  >  NC_000913/4622768‑4623245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑