Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,941,942	C→T	noncoding (135/1542 nt)	rrsC →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,941,942	0	C	T	100.0%	25.9 / NA	12	noncoding (135/1542 nt)	rrsC	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/3);  total (9/3)

CGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCAC  >  NC_000913/3941703‑3942179                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              cgaagatccggacttccaaCGTCCCAAGATGAAAAATCCATACCAAGTCTCAAGAGTGAAGACGAAATTCCTTACGAAGTTTCATTCTTTGACCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGACTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGAGCGTAGTGGCTGGGACACTGTCTgctgcgggg                                                                                                                                                                                                                                    >  2:18808/18‑243 (MQ=1)                                                                                            aGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCCGATGTGccc                                                                                                                                           >  1:143129/1‑249 (MQ=14)                                                                                                tCAAACTTTTAAATTGCAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGGGCGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTTCTGGAAACGGTAGCTAATATCGCATAACGTTGGAAGACCCATGAGGGGGACCTTCGGGCCTCTTTCCATCAGCTGTGCCCAGATgg                                                                                                                                     >  2:17525/1‑251 (MQ=11)                                                                                                                      ttGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTa                                                                                                                >  1:150582/1‑250 (MQ=37)                                                                                                                       tGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGAACTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGGAGGGGGGGTAAc                                                                                                              >  2:167111/1‑251 (MQ=255)                                                                                                                         aTCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGAAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACATTCGGGCCTCTTGCCATCAGATGTGCACAGATGGGGTTAGCTAGTAGGTGGGGTAACgg                                                                                                            >  2:115144/1‑251 (MQ=255)                                                                                                                                 tCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATTTGCCCAGATGGGATTAGCTAGTAGGTGGGGTACCGGCTCACCtt                                                                                                    >  2:159635/1‑250 (MQ=255)                                                                                                                                                        ccTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTg                                                                             <  1:167111/251‑1 (MQ=255)                                                                                                                                                                                   aGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGCCTGGGAAACTGTTTGATGGAGGGGGATAACTACTGGAGACGGGAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGCGCCTCTTGCCCTCGGATGTGCCCCGCTGGGATTAGCTCGGATGTCGGGTAACGGCTCACCTATGCGACGATCCCTAGCCGGTCTGTGCGGATGCCCCTCCGCACTGGAACtc                                                  >  2:25717/1‑250 (MQ=11)                                                                                                                                                                                                cTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGccc                                                                                                                                           >  1:167259/1‑149 (MQ=255)                                                                                                                                                                                                cTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGccc                                                                                                                                           <  2:167259/149‑1 (MQ=255)                                                                                                                                                                                                                                   gTCTGGGAAACTGTATGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGAACCTTCGGGCCTCTTGACATAAGATGTGCACAGATGGGATTAGCTAGTATGAGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGGCCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGcac  <  2:225119/251‑1 (MQ=11)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              CGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCAC  >  NC_000913/3941703‑3942179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑