Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,522,135	C→T	V115I (GTT→ATT)	xapR ←	DNA‑binding transcriptional activator XapR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,522,135	0	C	T	100.0%	93.9 / NA	30	V115I (GTT→ATT)	xapR	DNA‑binding transcriptional activator XapR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/12);  total (18/12)

CTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAACCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGGAGGCTG  >  NC_000913/2521904‑2522375                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 cTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATAGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCcac                                                                                                                                                                                                                               >  2:263953/1‑251 (MQ=255)    ccAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACAt                                                                                                                                                                                                                             <  1:151658/250‑1 (MQ=255)                 ggAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTGTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCTCATTGCCGTTCCCACTa                                                                                                                                                                                                               <  1:12702/251‑1 (MQ=255)                   aaaaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAAACGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACg                                                                                                                                                                                                             >  1:179082/1‑251 (MQ=255)                        gctgaGAGATGATGCTCTTCAGGAATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCACGAAAAAGAACGTCAACGTTATGACTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCg                                                                                                                                                                                                          >  2:225946/3‑249 (MQ=255)                                   atgCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCACTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGcc                                                                                                                                                                                             >  2:81322/1‑251 (MQ=255)                                   atgCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCGGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTGCCCACTACGCCGAGTTCAATCCGcc                                                                                                                                                                                             <  2:156350/251‑1 (MQ=255)                                    tgCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGccc                                                                                                                                                                                            >  2:10844/1‑251 (MQ=255)                                                    ccaccaGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCAAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCt                                                                                                                                                                            >  2:129824/1‑251 (MQ=255)                                                                     tCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAg                                                                                                                                                            <  2:281498/250‑1 (MQ=255)                                                                      cATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGcc                                                                                                                                                          >  1:151180/1‑251 (MQ=255)                                                                          cAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATa                                                                                                                                                      <  2:249486/251‑1 (MQ=255)                                                                                   gcgggTAAAACCCGGCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCCTGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAAAACGTCAACGTTAGGATTTCCCCTGAGGAATCGCCGCATAATCGTGCGCATCCGTCCCCACATTGCCGTTCCCACTCCTCCGGGTTCAATCCTCCCTGCTTCCCCCCGACCTATTTGTCCAATCCGAGCCAATACAttattat                                                                                                                                             <  2:244353/248‑2 (MQ=255)                                                                                        tAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTc                                                                                                                                        >  2:55485/1‑251 (MQ=255)                                                                                         aaaaCCAGGCGGTGGTTCTGTGGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCAATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATGGCCGTTCCCACTCCGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCa                                                                                                                                       <  2:86739/251‑1 (MQ=255)                                                                                           acccattcgttggtTCTGTCGGCAATCGCCAGATCCCGGCATCAATTTCGCGGCGTTCCATCAAGGCCATTTGCAGCGCTGGCATCTTTGCGCGAAAAAGAATGTTATCGTTAGGGTATGCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGATTCCAATCCGCCCTGCTTCTCCCCGTCCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTctcc                                                                                                                                     <  2:93292/241‑4 (MQ=255)                                                                                                 gcGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAAt                                                                                                                               <  2:156212/250‑1 (MQ=255)                                                                                                                 cATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCa                                                                                                               <  1:10844/251‑1 (MQ=255)                                                                                                                          gATCCCGGCATCAAGTTCGCGGCGTTCCAACAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCACCGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGCGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGAttt                                                                                                      >  2:186951/1‑251 (MQ=255)                                                                                                                                              ggcgTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTacg                                                                                  >  1:157963/1‑251 (MQ=255)                                                                                                                                                          aGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAAGCGCCGCATAATCGGGCGCATCCGTCCCCACATGGCCGTTCCCACTCCGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCGCGCGTGTGTCAGTACGACGCTGCGCGaa                                                                      <  2:151180/251‑1 (MQ=255)                                                                                                                                                                    cATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATa                                                             >  1:92644/1‑250 (MQ=255)                                                                                                                                                                    cATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATa                                                             >  2:61274/1‑250 (MQ=255)                                                                                                                                                                        gctggCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGATTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCGCCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAg                                                        <  1:263953/248‑1 (MQ=255)                                                                                                                                                                                         aaaaaGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGAt                                       >  1:26822/1‑251 (MQ=255)                                                                                                                                                                                              gAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTACCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTcc                                  >  2:100877/1‑251 (MQ=255)                                                                                                                                                                                               aaCGTCAACGTTAGGATTTTCCCTGCGGACTCGCCGCCTAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTcc                                  >  2:13988/1‑250 (MQ=255)                                                                                                                                                                                                                 ttCCCTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGc               >  1:174927/1‑251 (MQ=255)                                                                                                                                                                                                                   cccTGAGGAATCGCCGCATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGACAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTg             >  2:83543/1‑251 (MQ=255)                                                                                                                                                                                                                                ccgcATAATCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGATTAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGGAGGCTg  >  2:52430/1‑249 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 CTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTGCATCGCGGGCATCTTTTCGCGAAAAAGAACGTCAACGTTAGGATTTTCCCTGAGGAATCGCCGCATAACCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTTCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGGAGGCTG  >  NC_000913/2521904‑2522375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑