Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 34,178 G→A intergenic (+140/‑122) carB → / → caiF carbamoyl‑phosphate synthetase large subunit/DNA‑binding transcriptional activator CaiF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091334,1780GA100.0% 64.2 / NA 19intergenic (+140/‑122)carB/caiFcarbamoyl‑phosphate synthetase large subunit/DNA‑binding transcriptional activator CaiF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/11);  total (8/11)

ATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGCGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAATTGCTTCAAAACGAGTCATAGCCAGACTTTTAATTTGTGAAACTGGAGTTCGTATGTGTGAAGGATATGTTGAAAAACCACTCTACTTGTTAATCGCCGAATGGATGATGGCTGAAAATCGGTGGGTGATAGCAAGAGAGATCTCTATTCATTTCGATATTGAACACAGCA  >  NC_000913/33932‑34417
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
aTTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGCGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAACTca                                                                                                                                                                                                                                             <  1:255802/251‑1 (MQ=255)
     ggcaccacACTGAACGGCAGCTGTGCCACCGCGATGGCACTGCATGCCAAGGCGGCTGAAAAAGTAATTTGTGTTCAGGAAATTCCCGCACAGATCAAAGAAGTGCGTCTAATTGCAGATATTTTTCATCCGCTGATTTGGTCGAATAACGAATACGGTTCTCTTATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCGTTTGGAGAATCGATATATTTGATAACATAAGCAGTATTTAACTCACACtt                                                                                                                                                                                                                                        <  2:31251/249‑1 (MQ=255)
               tGAACGGCGGCTTTGCCACCGCGATGGCGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGTTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATACGCAGGATTTAACTCACACTTATCGACGGTg                                                                                                                                                                                                                              >  2:241780/1‑251 (MQ=255)
               tGAACGGCGGCTTTGCCACCGCGATGGCGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGCCCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAACTCACACTTATCGACGGTg                                                                                                                                                                                                                              >  1:202703/1‑251 (MQ=255)
                   cggcggCTTTGCCACCGCGATGGCGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAACTCACACTTATCGACGGTGAAGt                                                                                                                                                                                                                          <  1:225742/251‑1 (MQ=255)
                      cggcTTTGCCACCGCGATGGCGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAACTCACACTTATCGACGGTGAAGTTGc                                                                                                                                                                                                                       >  1:47425/1‑251 (MQ=255)
                                 ccGCGATGGCGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAACTCACACTTATCGACGGTGAAGTTGCATACTATCGat                                                                                                                                                                                                            >  2:58809/1‑251 (MQ=255)
                                     gagngcgcTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGGAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAACTCACACTTATCGACGGTGAAGTTGCATACTATCGatatat                                                                                                                                                                                                        <  2:256228/247‑1 (MQ=255)
                                                                aaaaGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAACTCACACTTATCGACg                                                                                                                                                                                                                                 >  1:121699/1‑199 (MQ=255)
                                                                aaaaGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAACTCACACTTATCGACg                                                                                                                                                                                                                                 <  2:121699/199‑1 (MQ=255)
                                                                                    aaaTGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATCTTTTTGATGACATAAGCAGGATTTAACTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAATTGCTTCAAAACGAGTCAt                                                                                                                                                          >  2:75412/1‑250 (MQ=255)
                                                                                                               gtgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAACTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAATTGCTTCAAAACGAGGCATAGCCAGACTTTTAATTTGTGAAACTGGa                                                                                                                              <  1:146571/251‑1 (MQ=255)
                                                                                                                             atTTTTCATCCGCTAATTTGATCGAATAACTAATGCGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAACTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAATTGCTTCAAAACGAGTCATAGCCAGACTGTTAATTTGTGAAACTGGAGTTCGTAtgtgtg                                                                                                                 <  1:263277/250‑1 (MQ=255)
                                                                                                                                                                           tgaGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAACTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAATTGCTTCAAAACGAGTCATAGCCAGACTTTTAATTTGTGAAACTGGAGTTCGTATGTGTGAAGGATATGTTGAAAAACCACTCTACTTGTTAATCGCCGAATGgatg                                                                  >  1:70609/1‑251 (MQ=255)
                                                                                                                                                                                     ttttttttGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAACTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAATTGCTTCAAAACGAGTCATAGCCAGACTTTTAATTTGTGAAACTGGAGTTCGTATGTGTGAAGGATATGTTGAAAAACCACTCTACTTGTTAATCGCCGAATGGATGATGGCTGaa                                                         <  1:201852/250‑1 (MQ=255)
                                                                                                                                                                                      tttttttACCCATGAAGTAACTCTTTTGTGGAATCGATATTTTTGATGACATAATCAGGATTTAACTCACTCTTAGCGACGGTGAAGTTGCATACTATCGATATATCCCCAATTTTAATAGGGCCTTGTTTAATTGCTTCAAAACGAGTCATAGCCAGACTTTTAATTTGTGAAACTGGAGTTCGTATGTGTGAAGGATATGTTGAAAAACCTCTCGACTTGTTAATCGCCGAATGGATGATGTCTGaaa                                                        <  2:192617/250‑1 (MQ=255)
                                                                                                                                                                                                                        cGATATTTTTGATGACATAAGCAGGATTTAACTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAATTGCTTCAAAACGAGTCATAGCCAGACTTTTAATTTGTGAAACTGGAGTTCGTATGTGTGAAGGATATGTTGAAAAACCACTCTACTTGTTAATCGCCGAATGGATGATGGCTGAAAATCGGTGGGTGATAGCAAGAGAGATCTCTATTCa                      <  1:161687/250‑1 (MQ=255)
                                                                                                                                                                                                                                   atgaCATAAGCAGGATTTAACTCACACTTATCGACGGGGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAATTGCTTCAAAACGAGTCATAGCCAGACTTTTAATTTGTGAAACTGGAGTTCGTATGTGTGAAGGATATGTTGAAAAACCACTCTACTTGTTAATCGCCGAATGGATGATGGCTGAACATCGGTGGGTAATAGCAAGAGCGATCTCTATTCATTTCGATATTg           >  2:71778/1‑250 (MQ=255)
                                                                                                                                                                                                                                           aGCAGGATTTAACTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAATTGCTTCAAAACGAGTCATAGCCAGACTTTTAATTTGTGAAACTGGAGTTCGTATGTGTGAAGGATATGTTGAAAAACCACTCTACTTGTTAATCGCCGAATGGATGATGGCTGAAAATCGGTGGGTGATAGCAAGAGAGATCTCTATTCATTTCGATATTGAACACAGCa  <  2:47425/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
ATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGCGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAATTGCTTCAAAACGAGTCATAGCCAGACTTTTAATTTGTGAAACTGGAGTTCGTATGTGTGAAGGATATGTTGAAAAACCACTCTACTTGTTAATCGCCGAATGGATGATGGCTGAAAATCGGTGGGTGATAGCAAGAGAGATCTCTATTCATTTCGATATTGAACACAGCA  >  NC_000913/33932‑34417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑