Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,521,000	C→T	noncoding (70/76 nt)	valU →	tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,521,000	0	C	T	100.0%	40.7 / NA	16	noncoding (70/76 nt)	valU	tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/7);  total (9/7)

TGATTCCTCAATGTAGTTCGGGTTTCTACCTTACGCATTATTGTTTTCGTTGACAAATTGCGCAACACGGTCTGTTTTGCGATCATCTTGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCA  >  NC_000913/2520755‑2521238                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               tGATTCCTCAATGTAGTTCGGGTTTCTACCTTACGCATTATTGTTTTCGTTGACAAATTGCGCAACACGGTCTGTTTTGCGATCATCTTGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcatccacc                                                                                                                                                                                                                                           >  1:278875/1‑251 (MQ=255)    ttCCTCAATGTAGTTCGGGTTTCTACCTTACGCATTATTGTTTTCGTTGACAAATTGCGCAACACGGTCTGTTTTGCGATCATCTTGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCATCCCCCAac                                                                                                                                                                                                                                        >  1:9234/1‑251 (MQ=255)                             ccTTACGCATTATTGTTTTCGTTTACAAATTGCGCAACACGGTCTGTTTTGCTATCATCTTGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATGAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCATCCACCAACTGCTTTATGTAGTCGCCGCCGTGTa                                                                                                                                                                                                               <  2:171868/251‑1 (MQ=255)                                tACGCATTATTGTTTTCGTTGACAAATTGCGCAACACGGTCTGTTTTGCGATCATCTTGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCATCCACCAACTACTTTATGTAGTCTCCGCCGTGTAg                                                                                                                                                                                                              <  2:278527/249‑1 (MQ=255)                                tACGCATTATTGTTTTCGTTGACAAATTGCGCAACACGGTCTGTTTTGCGATCATATTGCTAAAATTTACGACGAACGAAAACTTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCATCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGCTCCCGTCATCATCCAACAACAAATTTATgcagcatacgccgagtaacc                                                                                                                                                                                                            >  1:98950/1‑232 (MQ=37)                                 aCGCATTATTGTTTTCGTTGACAAATTGCGCAACACGGTCTGTTTTGCGATCATCTTGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCATCCACCAACTACTTTATGTAGTCTCCGCCGTGTAgaaa                                                                                                                                                                                                           <  2:278875/251‑4 (MQ=255)                                         ttGTTTTCGTTGACAAATTGCGCAACACGGTCTGTTTTGCGATCATCTTGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCATCCACCAACTACTTTCTGTAGTTTACGTCGTGTAGCAAgtaacttt                                                                                                                                                                                                   >  1:34427/1‑244 (MQ=38)                                                  ttGACAAATTGCGCAACACGGTCTGTTTTGCGATCATCTTGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCATCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTgggg                                                                                                                                                                                          >  1:77506/1‑250 (MQ=255)                                                                            tttGCGATCATCTTGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCATCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTc                                                                                                                                                                >  1:28294/1‑251 (MQ=255)                                                                                cGATCATCTTGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCATCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGAACCTcccct                                                                                                                                                            >  1:134161/1‑249 (MQ=255)                                                                                                aTTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCATCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTTAGAATTGGGTGATTAGATCAGCTGGTAGAGCACCACCCTTTCAAGGAGGGGGTcgg                                                                                                                                            >  2:234767/1‑251 (MQ=38)                                                                                                         gaacgaacAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCATCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGTTTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGGTCggt                                                                                                                                   >  1:271659/1‑249 (MQ=255)                                                                                                                  aTTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCATCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtcatc                                                                                                                          <  1:215588/251‑1 (MQ=255)                                                                                                                            aaaaTGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCATCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACt                                                                                                                <  1:249056/251‑1 (MQ=255)                                                                                                                                     tGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGCGGGGGTCGGCGGTTCCAACCCGTCATCATCCACCAACTTCTTTCTGTAGTCTCCGCCGTTTAGCAAGTAATTAAGAAGTGAGTGATTAGCGCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTTGATCCCCTCATcacccccccccttatcggc                                                                                                        >  1:44146/1‑236 (MQ=37)                                                                                                                                                                                             cAGCTGGGAGAGCACCTCCCTGACAAGGAGTGGGTCGGCGGTTCGATCCCGTCATCATCCACCAACTACTTTATTTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGTGGGTGATTAGCTCAGCTGGga                                               <  2:28294/251‑1 (MQ=255)                                                                                                                                                                                                                                            cccGTCATCATCCACCATCTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGATCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCGCCCACGACTTTCTCGCCATCTAAATTTCTTGTAAAAATGTGAAGTTCCGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtca  <  1:131117/249‑1 (MQ=38)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               TGATTCCTCAATGTAGTTCGGGTTTCTACCTTACGCATTATTGTTTTCGTTGACAAATTGCGCAACACGGTCTGTTTTGCGATCATCTTGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCA  >  NC_000913/2520755‑2521238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑