Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,381,273	C→T	V91M (GTG→ATG)	elaA ←	putative acetyltransferase ElaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,381,273	0	C	T	100.0%	68.7 / NA	21	V91M (GTG→ATG)	elaA	putative acetyltransferase ElaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/12);  total (9/12)

ATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCATCTTCTTCATAGACCTCCGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCACTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAAC  >  NC_000913/2381026‑2381504                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        aTTCTCTTTCTCCATTTGCTTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCATCTTCTTCATAGACCTCCGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCCCCTCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTTCATGTTTCGAGTGTTTTACTCATCATCTGCTGCCCCAtttt                                                                                                                                                                                                                                      <  2:194577/251‑1 (MQ=255)   tctcGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCATCTTCTTCATAGACCTCCGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCAtttttt                                                                                                                                                                                                                                    >  1:42905/1‑251 (MQ=255)                                   aactaTAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCATCTTCTTCATAGACCTCCGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGATGCCGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCg                                                                                                                                                                                                     <  2:1178/250‑1 (MQ=255)                                           aCAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCATCTTCTTCATAGACCTCCGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAAc                                                                                                                                                                                            <  1:246014/251‑1 (MQ=255)                                           aCAAGGGCTGTTGATTCCGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCATCTTCTTCATAGACCTCCGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATATTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCTCCACGCAACGCTTCCCTGACAATCACCCGACCTATAAc                                                                                                                                                                                            >  2:199511/1‑251 (MQ=255)                                                       gagttcGCCTGAATTACCCCGCGTGCCATCCCAATGTGTGGGATACCATCTTCTTCATAGACCTCCGTCACAGGGATAAGCCCGAAACTCTGGTAGAAGGTTTGCAGATGCGCCTGCGCCCCGAGGTAAGCAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCGGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGTCCGGCTCaa                                                                                                                                                                                 <  2:166887/245‑1 (MQ=255)                                                          tACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCATCTTCTTCATAGACCTCCGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGat                                                                                                                                                                              <  1:274333/250‑1 (MQ=255)                                                           aCGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCATCTTCTTCATAGACCTCCGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGatc                                                                                                                                                                             <  1:133628/250‑1 (MQ=255)                                                             gCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCATCTTCTTCATAGACCTCCGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGGGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCTCTGACAATCACCCGACCTATAACGACCGGCTCCAGatcatc                                                                                                                                                                          >  2:7254/1‑251 (MQ=255)                                                                tGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCATCTTCTTCATAGACCTCCGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGatcatcatc                                                                                                                                                                       >  1:2226/1‑251 (MQ=255)                                                                                                    ccATCTTCTGCATGGACCTACGTCACGGGGATGAAACCGAAACTCTGGTAGAAGTTTTGCAGTGGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTTCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTGCAGAATCCTCTCATACGCCGCCAGTtcatc                                                                                                                                   <  2:188524/251‑1 (MQ=255)                                                                                                            ttcATAGACCTCCGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTcc                                                                                                                           >  2:15560/1‑251 (MQ=255)                                                                                                                       ccGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAat                                                                                                                 <  1:141429/250‑1 (MQ=255)                                                                                                                       ccGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTGTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAata                                                                                                                <  1:29451/251‑1 (MQ=255)                                                                                                                                     aaaCCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATcccc                                                                                                  >  1:144741/1‑251 (MQ=255)                                                                                                                                       aCCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCTACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGTCGATTATCCCCCg                                                                                                <  2:125201/251‑1 (MQ=255)                                                                                                                                                 tGGTAGAAGCTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTCCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCACCCGAGAATATGGCGATTATCCCCCGTCAAatcatc                                                                                      >  2:95273/1‑251 (MQ=255)                                                                                                                                                       aaGTTTTGCATATGCGCCTGCGCCCCGAGGTTAACAGGCTTATCAGGCCAGTGATGCGTTCATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCATAATCCTCGCATACGCCCCCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATc                                                                                <  1:21840/251‑1 (MQ=255)                                                                                                                                                                                                       cAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACg                                >  1:16247/1‑251 (MQ=255)                                                                                                                                                                                                              gCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTg                          <  2:16247/250‑1 (MQ=255)                                                                                                                                                                                                                     tGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAgg                  >  2:109253/1‑251 (MQ=255)                                                                                                                                                                                                                                     cTCATCAGCTGCTGCCCCATTTTTTCGCCACGCAACGATTAACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGAATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGAGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAAc  >  2:264170/1‑251 (MQ=255)                                                                                                                                                                                                                                       catcaGCTGCTGCCCCATTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAAc  <  1:102430/249‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        ATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCATCTTCTTCATAGACCTCCGTCACAGGGATAAAACCGAAACTCTGGTAGAAGTTTTGCAGATGCGCCTGCGCCCCGAGGTAAACAGGCTTATCAGGCCAGTGATGCGTACATGTTTCGAGTGTTTTACTCATCAGCTGCTGCCCCACTTTTTCGCCACGCAACGCTTCACTGACAATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAAC  >  NC_000913/2381026‑2381504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑