Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	661,919	C→T	A120A (GCG→GCA)	lipB ←	lipoyl(octanoyl) transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	661,919	0	C	T	100.0%	65.2 / NA	20	A120A (GCG→GCA)	lipB	lipoyl(octanoyl) transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/8);  total (12/8)

AGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGATATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGCGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGGTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTG  >  NC_000913/661674‑662128                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                  agCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGATCTTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATTCGTAAAAATGGTGAAAGATCCATATTGCCGTTTGATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCATTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTc                                                                                                                                                                                                              <  2:148487/251‑1 (MQ=255)                        aCGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGATATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCATCATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTAt                                                                                                                                                                                       <  1:282493/251‑1 (MQ=255)                                             cgtcgCTTCGGGTTTCCATTGTGATATTTTAGACATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTcaagc                                                                                                                                                                   >  1:107044/1‑250 (MQ=255)                                                        gTTTCCATTGTGATATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGt                                                                                                                                                       >  1:267160/1‑251 (MQ=255)                                                          ttCCATTGTGATATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGCCGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTc                                                                                                                                                     >  2:64553/1‑251 (MQ=255)                                                                         ttAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAGCCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGtt                                                                                                                                       <  2:223646/250‑1 (MQ=255)                                                                           aGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAATGTCACCAGTTCACGCACCCCGAGTTTAc                                                                                                                                    <  1:235033/251‑1 (MQ=255)                                                                                     aTTCCGGCATACACACCAGGATTAATACGTAAAAATCGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCCTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCGGGCGCGTCGGCCCGAGGATGTTCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCTAAGTCACCAGATCAAGCACACCGAGTTTACGGCGttac                                                                                                                            >  2:130601/1‑247 (MQ=255)                                                                                               aCCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTc                                                                                                                                                             <  1:64314/206‑1 (MQ=255)                                                                                               aCCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTc                                                                                                                                                             >  2:64314/1‑206 (MQ=255)                                                                                                   acaAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGGTTAAGCAACacat                                                                                                            <  2:14300/251‑1 (MQ=255)                                                                                                                   aaaaaTGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGGTTAAGCAACACATACATCACCTGTTGccc                                                                                            <  2:229098/251‑1 (MQ=255)                                                                                                                     aaaTGGCGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGGATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCATTTCACGCACACCGAGTTTACGGCGTTTCAGGTTAAGCAACACATACATCACCTGTTGCCCCg                                                                                          >  2:249595/1‑251 (MQ=255)                                                                                                                       aTGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGGTTAAGCAACACATACATCACCTGTTGCCCCGGc                                                                                        >  1:21044/1‑251 (MQ=255)                                                                                                                                      tatTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGGTTAAGCAACACATACATCAACTGTTGCCCCGGCCCGTGATAAGTCAcc                                                                         >  1:253299/1‑251 (MQ=255)                                                                                                                                           aCGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGGTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGccc                                                                    >  1:152203/1‑251 (MQ=255)                                                                                                                                             gTTTAATGCCAGACAGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGAAAGGTCACCAGTTCACGCACACCGCGTTTACGGCGTTTCAGGTTAAGCAACACATACATCACCTGTTGCCCCGGCACGTGATAAGTCACCTGCCCAc                                                                  >  1:204105/1‑251 (MQ=255)                                                                                                                                                    gCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGGTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGAt                                                            <  2:94435/250‑1 (MQ=255)                                                                                                                                                           cGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGGTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTgg                                                    >  2:197819/1‑251 (MQ=255)                                                                                                                                                                                                             ttttcCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGTGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGGTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTcctgcctg  >  2:44972/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                  AGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGATATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTGAGCAAATTTTCTTTTCCCCAACATAGACACCTGGCGCGTCAGCCCGAGGATGCGCTTCTATACCCAGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGGTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTG  >  NC_000913/661674‑662128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑