Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,675,265	C→T	G166E (GGA→GAA)	yphC ←	putative zinc‑binding dehydrogenase YphC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,675,265	0	C	T	100.0%	74.3 / NA	22	G166E (GGA→GAA)	yphC	putative zinc‑binding dehydrogenase YphC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/15);  total (7/15)

CGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGCTCAGCGCATCCGGCAGGAGGATCAGATCTTTTTCTTCCGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTC  >  NC_000913/2675021‑2675494                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      ggcAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGGTTTGCACCGCGACCTTTCGCCAGCATCATCGCCGTCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCAt                                                                                                                                                                                                                                 <  1:179504/250‑1 (MQ=255)    aaCATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATAcc                                                                                                                                                                                                                              >  2:79361/1‑250 (MQ=255)       agccGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTg                                                                                                                                                                                                                           <  1:173431/249‑1 (MQ=255)         ccGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGt                                                                                                                                                                                                                          <  2:112575/250‑1 (MQ=255)             gcCACCGTGGGTGCGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAACTTTCTTCATACGCTGTACCa                                                                                                                                                                                                                      >  2:125663/1‑250 (MQ=255)                  cGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGTAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAAcgccg                                                                                                                                                                                                                <  1:1261/251‑1 (MQ=255)                      ggTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAAc                                                                                                                                                                                                            <  1:27465/251‑1 (MQ=255)                                          gcagCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCCCCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCATCGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCtt                                                                                                                                                                                          <  1:255366/246‑1 (MQ=255)                                           cGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGt                                                                                                                                                                                       <  1:252881/251‑1 (MQ=255)                                              cAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGc                                                                                                                                                                                    <  1:134845/251‑1 (MQ=255)                                                                                        tAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGCTCAGCGCATCCGGCAGGAGGATCAGATCTTTTTCTTCCGcc                                                                                                                                           >  1:96115/1‑250 (MQ=255)                                                                                         aaCTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGCTCAGCGCATCCGGCAGGAGGATCAGATCTTTTTCTTCCGCCAg                                                                                                                                         <  1:190862/251‑1 (MQ=255)                                                                                                 tGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGCTCAGCGCATCCGGCAGGAGGATCAGATCTTTTTCTTCCGCCAGCAAGTAt                                                                                                                                  <  2:96115/250‑1 (MQ=255)                                                                                                                   cGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGCTCAGCGCATCCGGCAGGAGGATCAGATCTTTTTCTTCCGCCAGCAAGTATTCGGCATGACCGCCGTCAc                                                                                                               >  1:153821/1‑251 (MQ=255)                                                                                                                       agcaTATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGCTCAGCGCATCCGGCAGGAGGATCAGATCTTTTTCTTCCGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTg                                                                                                           >  1:203917/1‑251 (MQ=255)                                                                                                                                           ccGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGCTCAGCGCATCCGGCAGGAGGATCAGATCTTTTTCTTCCGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTc                                                                                       >  2:73660/1‑251 (MQ=255)                                                                                                                                                  gCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGCTCAGCGCATCCGGCAGGAGGATCAGATCTTTTTCTTCCGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGcc                                                                                >  2:272255/1‑251 (MQ=255)                                                                                                                                                         gACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGCTCAGCGCATCCGGCAGGAGGATCAGATCTTTTTCTTCCGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACaa                                                                         <  1:1814/251‑1 (MQ=255)                                                                                                                                                                 gCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGCTCAGCGCATCCGGCAGGAGGATCAGATCTTTTTCTTCCGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAgg                                                                 <  2:203917/251‑1 (MQ=255)                                                                                                                                                                                                                     tATCCCTGCCGCAGACTTCGCCGCGCAAAATTTCTTCATACTCTGTACCACCGCCGCCACTGAAAACCGCACCATCTTCGTAGCTAGGCGCATCCGGAAGGAGGATCAGATCTTTTCCTTACGCAAGCACGTATTAGGCAGGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAAGAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACCGCCAGAAATGTCATacac              <  2:43839/250‑1 (MQ=255)                                                                                                                                                                                                                         ccTGCCGGAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGCTCAGCGCATCCGGCAGGAGGATCAGATCTTTTTCTTCCGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCa         <  1:29353/250‑1 (MQ=255)                                                                                                                                                                                                                                gAAACTTCGCCGCGCAAAATTTCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGCTCAGCGCATCCGGCAGGAGGATCAGATCTTTTTCTTCCGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTc  <  1:79361/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      CGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTCGTAGCTCAGCGCATCCGGCAGGAGGATCAGATCTTTTTCTTCCGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTC  >  NC_000913/2675021‑2675494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑