Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,448,413	C→T	V10I (GTA→ATA)	prmB ←	ribosomal protein L3 N(5)‑glutamine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,448,413	0	C	T	100.0%	70.5 / NA	24	V10I (GTA→ATA)	prmB	ribosomal protein L3 N(5)‑glutamine methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/18);  total (6/18)

AAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCGCGGTGAGTGAGGAAAAATACGTTTAAAACGATCGATTGCGCTACGAGTCGGGTAAACTGTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGA  >  NC_000913/2448167‑2448637                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 aaGCCACCGTAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGc                                                                                                                                                                                                                               <  2:199729/250‑1 (MQ=255)      aCCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTtcatc                                                                                                                                                                                                                         <  1:58243/251‑1 (MQ=255)            aggCGTTCATTGACGCGTCGGGTCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACACGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAACGGAAGCAACACCAGCTGTACGGCTTCATCCCAAGGGAGATCGGTACCGTGACCAGAACAGATATTTGCCGCGCGGAAGGAGCACCCC‑GGCCAGCACCACAAGTACTAAATGGGTGGCAGCTCCTTTATTGGTTAATCAACGa                                                                                                                                                                                                                    >  2:229295/3‑250 (MQ=255)                       gACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATcc                                                                                                                                                                                                         <  1:239452/250‑1 (MQ=255)                               ggATCACGCGTTCAACAATAAGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTTGAGCGCAGGCAACAACAGCTCTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTCCCGCGCTGAAGCGGC‑CACCCGACCAGCGCAACATGACCTGGATGGTTTGCAGCTCATTTATTGTCTCATCAACGAAAATTTTATCCACCTATtcc                                                                                                                                                                                                >  2:283465/1‑251 (MQ=255)                                              caaTACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCg                                                                                                                                                                                 >  1:152690/1‑251 (MQ=255)                                                                                 gTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTTTGCCATGAAGATGa                                                                                                                                              <  2:121621/251‑1 (MQ=255)                                                                                      cATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTGTGCCATGAAGATGACGATa                                                                                                                                         <  1:206246/251‑1 (MQ=255)                                                                                                           cGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTTTGCCATGAAGATGACGATAAATAAGCATTCACGCGCGgtg                                                                                                                    >  2:79558/1‑251 (MQ=255)                                                                                                            ggCAGGTAGAGCGAATGCAACACTAGCTGTACGGCTTCATCCCGCGGGTTATCGGTACCGTGACCGTACCAGATATGTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTTGGCCATGAAGATGACGATAAATCAGCATTCACGCGCGgtga                                                                                                                   <  2:154281/251‑1 (MQ=255)                                                                                                            ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACg                                                                                                                                                                                                                     <  1:25325/153‑1 (MQ=255)                                                                                                            ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACg                                                                                                                                                                                                                     >  2:25325/1‑153 (MQ=255)                                                                                                                           ggCAACACCAGATGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGTGCATGATCGCAATAATTTCGGCGGCTAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCTCGGTGAGTGAGAAAAAATACg                                                                                                    <  2:113085/251‑1 (MQ=255)                                                                                                                                  ccAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCGCGGTGAGTGAGGAAAAATACGTTTaaaa                                                                                             <  1:93277/251‑1 (MQ=255)                                                                                                                                                     ccACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCGCGGTGAGTGAGGAAAAATACGTTTAAAACGATCGATTGCGCTActag                                                                          <  2:100019/251‑4 (MQ=255)                                                                                                                                                         gggTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCGCGGTGAGTGAGGAAAAATACGTTTAAAACGATCGATTGCGCTACGAGTCgg                                                                      <  2:268817/251‑1 (MQ=255)                                                                                                                                                                          aCCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCGCGGTGAGTGAGGAAAAATACGTTTAAAACGATCGATTGCGCTACGAGTCGGGTAAACTGTAGGAAAAt                                                     <  1:272905/251‑1 (MQ=255)                                                                                                                                                                           ccGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCGCGGTGAGTGAGGAAAAATACGTTTAAAACGATCGATTGCGCTACGAGTCGGGTAAACTGTAGGAAAAtt                                                    <  2:245689/251‑1 (MQ=255)                                                                                                                                                                                 cAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCGCGGTGAGTGAGGAAAAATACGTTTAAAACGATCGATTGCGCTACGAGTCGGGTAAACTGTAGGAAAATTAGAAAt                                              <  1:133275/251‑1 (MQ=255)                                                                                                                                                                                            cgcgCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCGCGGTGAGTGAGGAAAAATACGTTTAAAACGATCGATTGCGCTACGAGTCGGGTAAACTGTAGGAAAATTAGAAATAGAGACAGAt                                    >  2:42218/1‑250 (MQ=255)                                                                                                                                                                                                       ggCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGTGGCTAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCGCGGTGAGTGAGGAAAAATACGTTTAAAACGATCGATTGCGCTACGAGTCGGGTAAACTGTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGa                        <  1:119554/251‑1 (MQ=255)                                                                                                                                                                                                             cc‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTATTGCTTCAACAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAAGAATTTCGGCGGCTAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCGCGGCGAGTGAGGAAAAATACGTTTAAAACGATCGATTGCGCTACGAGAAGGGTAAACTGTAGGAAAATTAGAAATAGATACAGATAAATGAAAAAGAAaacaa                  <  2:206026/251‑1 (MQ=255)                                                                                                                                                                                                                            aTGTCCTGAATGGTTTGCAGCTCATTTATTTCTTCATCAACTAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCGAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCGCGGTGAGTGAGGAAAAATACGTTTAAAACGATCGATTGCGCTAAGAGGTGGGTAAACTTTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCgaggag    <  2:19962/251‑1 (MQ=255)                                                                                                                                                                                                                              gTCCTGAATGGTTTGCAGCTCATTTATTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCGCGGTGAGTGAGGAAAAATACGTTTAAAACGATCGATTGCGCTACGAGTCGGGTAAACTGTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAggagga  <  1:260465/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 AAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACC‑GACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGTTTGCCATGAAGATGACGATAAATCAGCATTCACGCGCGGTGAGTGAGGAAAAATACGTTTAAAACGATCGATTGCGCTACGAGTCGGGTAAACTGTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGA  >  NC_000913/2448167‑2448637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑