Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	236,865	G→A	intergenic (+67/‑66)	dnaQ → / → aspV	DNA polymerase III subunit epsilon/tRNA‑Asp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	236,865	0	G	A	100.0%	52.7 / NA	16	intergenic (+67/‑66)	dnaQ/aspV	DNA polymerase III subunit epsilon/tRNA‑Asp
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/10);  total (6/10)

GCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGAAGCCCGTCTCGATCTGGTGCAGAAGAAAGGCGGAAGTTGCCTCTGGCGAGCATAAATACCTGTGAAAGGCGCTAAAAATAGCGACTTGGGCGATTTTTGCAGCAAACGATTCAAAAGATGAGAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAACGGAACACAACGCGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTATTCACTCATGAAAATGAGTTCAGAGAGCCGCAAGATTTTTAATTTTGCGGTTTTTTTGTATTTGAATTCCACCATTTCTCTGTTCAATGATTTTACT  >  NC_000913/236628‑237107                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   gCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGAAGCCCGTCTCGATCTGGTGCAGAAGAAAGGCGGAAGTTGCCTCTGGCGAGCATAAATACCTGTGAAAGGCGCTAAAAATAGCGACTTGGGCGATTTTTGCAGCAAACGATTCAAAAGATGAAAAAAACCGTTGAc                                                                                                                                                                                                                                       <  1:82081/251‑1 (MQ=255)           gagagaAACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGGAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGAAGCCCGTCTCGATCTGGTGCAGAAGAAAGGCGGAAGTTGCCGCTGGCGAGCAGAAATACCTGTGAAAGGCGCTAAAAATAGCGACTTGGGCGATTTTTGCAGCAAACGATTCAAAAGATGAAAAAAACCGTTGACGAATGTCGAg                                                                                                                                                                                                                             <  1:222149/251‑1 (MQ=255)                                  gAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGAAGCCCGTCTCGATCTGGTGCAGAAGAAAGGCGGAAGTTGCCTCTGGCGAGCATAAATACCTGTGAAAGGCGCTAAAAATAGCGACTTGGGCGATTTTTGCAGCAAACGATTCAAAAGATGAAAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGt                                                                                                                                                                                                      >  1:271033/1‑251 (MQ=255)                                                  gcATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGAAGCCCGTCTCGATCTGGTGCAGAAGAAAGGCGGAAGTTGCCTCTGGCGAGCATAAATACCTGTGAAAGGCGCTAAAAATAGCGACTTGGGCGATTTTTGCAGCAAACGATTCAAAAGATGAAAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAACGGACcaca                                                                                                                                                                                       >  2:99999/1‑250 (MQ=255)                                                          cGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGAAGCCCGTCTCGATCTGGTGCAGAAGAAAGGCGGAAGTTGCCTCTGGCGAGCATAAATACCTGTGAAAGGCGCTAAAAATAGCGACTTGGGCGATTTTTGCAGCAAACGATTCAAAAGATGAAAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAACGGAACACAACGCGGAGc                                                                                                                                                                              <  1:119852/251‑1 (MQ=255)                                                              aGGCAAGTAAGTTACGCGTTGTTATTGCGACAGCTGAAGTGATTGCAGCTCATGAAGCCCGTCTCGATCTGGTGCAGAATAAAAGCGGAAGTTGCCTCTGGCGCACATAAATACCTGTGAAAGGCGCTAAAAATAGCGACTTGGGCGATTTTTGAAGCAAACGATTCAAAAGATGAAAAAAACCGTTGACGAAGGTCGAGTCAATCCGTAATATTCGCCTCGTTCCCAACGGAACACAACGCGGAGcagt                                                                                                                                                                           >  2:108470/1‑247 (MQ=255)                                                                        gtTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGAAGCCCGTCTCGATCTGGTGCAGAAGAAAGGCGGAAGTTGCCTCTGGCGAGCATAAATACCTGTGAAAGGCGCTAAAAATAGCGACTTGGGCGATTTTTGCAGCAAACGATTCAAAAGATGAAAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAACGGAACACAACGCGGAGCGGTAGTTCAGTCgg                                                                                                                                                                <  2:242431/251‑1 (MQ=255)                                                                                                                     aGCCCGTCTCGATCTGGTGCAGAAGAAAGGCGGAAGTTGCCTCTGGCGAGCATAAATACCTGTGAAAGGCGCTAAAAATAGCGACTTGGGCGATTTTTGCAGCAAACGATTCAAAAGATGAAAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAACGGAACACAACGCGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTccc                                                                                                                   <  1:34364/251‑1 (MQ=255)                                                                                                                              cgagcTGGTGCAGAAGAAAGGCGGAAGTTGCCTCTGGCGAGCATAAATGCCTGTGAAAGGCGCTAAAAATAGCGACTTGGGCGATTTTTGCAGCAAACGATTCAAAAGATGAAAAAAACCGTTGAGGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAACGGAACACAACGCGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCccgtccgtgc                                                                                                           <  2:129685/246‑3 (MQ=255)                                                                                                                                                           gCCTCTGGCGAGCAAAAATACCTGTGAAAGGCGCTAAAAATAGCGACTTGGGGGATTTTTGCAGCAAACGATTCAAAAGATTAAAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAACGGAACACACCGCGGAGCGGTAGTGCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTATTCACTCATGAAAATGAGTTCa                                                                             <  1:74775/251‑1 (MQ=255)                                                                                                                                                                                               aaaaTAGCGACTTGGGCGATTTTTGCAGCAAACGATTCAAAAGATGAAAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAACGGAACACAACGCGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTATTCACTAATGAAAATGAGTTCAGAGAGCCGCACGATTTTTCATTTTGCGGTTTTTTTg                                         >  1:137979/1‑251 (MQ=255)                                                                                                                                                                                                 aagaGCGACTTGGGCAATTTTTGCAGCAACCGATTCAAAAGAGGAAAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAAAGGAACACAACGCGGAGCGTTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTATTCACTCATGAAAATGAGTTCAGAGAGCCGCAAGATTTTTAATTTTGCGGTTTTTTTGTa                                       <  1:142484/248‑1 (MQ=38)                                                                                                                                                                                                       gACTTGGGCGATTTTTGCAGCAAACGATTCAAAAGATGAAAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAACGGAACACAACGCGGAGCGGTAGTTCAGTCGGTTAGAATCCCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCAGTCCGTTCCGCCACTATTCACTCATGAAAATGAGTTCAGAGAGcc                                                                      <  2:231967/214‑1 (MQ=255)                                                                                                                                                                                                       gACTTGGGCGATTTTTGCAGCAAACGATTCAAAAGATGAAAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAACGGAACACAACGCGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTATTAACTCATGAAAATGAGTTCAGAGAGcc                                                                      >  1:231967/1‑214 (MQ=255)                                                                                                                                                                                                                       gcagcaAACGATTCAAAAGATGAAAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAACGGAACACAACGCGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTATTCACTCATGAAAATGAGTTCAGAGAGCCGCAAGATTTTTAATTTTGCGGTTTTTTTGTATTTGAATTCCACCATTTCTCTg                 <  1:42049/251‑1 (MQ=255)                                                                                                                                                                                                                                      aaaGATGAAAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAACGGAACACAACGCGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTATTCACTCATGAAAATGAGTTCAGAGAGCCGCAAGATTTTTAATTTTGCGGTTTTTTTGTATTTGAATTCCACCATTTCTCTGTTCAATGATTTTAct  >  1:105535/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   GCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGAAGCCCGTCTCGATCTGGTGCAGAAGAAAGGCGGAAGTTGCCTCTGGCGAGCATAAATACCTGTGAAAGGCGCTAAAAATAGCGACTTGGGCGATTTTTGCAGCAAACGATTCAAAAGATGAGAAAAACCGTTGACGAAGGTCGAGGCAATCCGTAATATTCGCCTCGTTCCCAACGGAACACAACGCGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTATTCACTCATGAAAATGAGTTCAGAGAGCCGCAAGATTTTTAATTTTGCGGTTTTTTTGTATTTGAATTCCACCATTTCTCTGTTCAATGATTTTACT  >  NC_000913/236628‑237107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑