Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	221,705	G→A	T314I (ACT→ATT)	metN ←	L‑methionine/D‑methionine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	221,705	0	G	A	100.0%	77.8 / NA	25	T314I (ACT→ATT)	metN	L‑methionine/D‑methionine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/12);  total (13/12)

GTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCACGAACCAGCAGCCACATCATCGGCTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAGTCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGATATCCAGATGCAGGGTCGACTGAATAAACTTCTGCGCCAGCGGCGTTTTC  >  NC_000913/221458‑221950                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gtaggatacagatacgagtgagtaataacTTGCCCCGTACACATGCCCTAAAGCGAAACACCAACCTGCATGCCAATCACAAAGCCAAAAGAACCGGATACGAAGGTCATCGCCAGCGGTTCCCACACCCCACGATCCAGCTGCCTCTCCATCGGCTCAGACATAACCCAGTACCTCTACTTTTGCATGGTGTTCCTGCAGCCAGGCAATGGCGCCTTGCGTATCTTGGTGTGTGCCTTTCATTtcaatca                                                                                                                                                                                                                                                     <  2:72212/228‑1 (MQ=255)           gCTTCGCGTTAGCAATAAGTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCACGAACCAGCAGCCACATCATCGGCTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTGCAATCAGCATGA‑TGcc                                                                                                                                                                                                                                          <  1:88392/251‑1 (MQ=255)                      gCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCACGAACCAGCAGCCACATCATCGGCTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGc                                                                                                                                                                                                                               >  1:63234/1‑251 (MQ=255)                                gCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGACAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCACGAACCAGCAGCCACATCATCGGCTCAGAAATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGGATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCggagt                                                                                                                                                                                                                      >  1:172899/1‑247 (MQ=255)                                        cgcgTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCACGAACCAGCAGCCACATCATCGGCTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAGCGCCACCGGCGTAATCCATCt                                                                                                                                                                                                             <  1:58311/251‑1 (MQ=255)                                        cgcgTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCACGAACCAGCAGCCACATCATCGGCTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGAGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCt                                                                                                                                                                                                             >  1:179348/1‑251 (MQ=255)                                           gTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCACGAACCAGCAGCCACATCATCGGCTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTgcg                                                                                                                                                                                                          >  2:111886/1‑251 (MQ=255)                                                                 ccGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGGCATTGCCAGCGTTTCCCATACGCCACGAACCAGCAGCCACATCATCGGCTCAGACATAACCCAGGACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCTGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGAGTGC‑GAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGAc                                                                                                                                                                                    <  1:16377/251‑1 (MQ=255)                                                                     cAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCACGAACCAGCAGCCACATCATCGGCTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTg                                                                                                                                                                                >  1:265628/1‑251 (MQ=255)                                                                                               cGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCACGAACCAGCAGCCACATCATCGGCTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGc                                                                                                                                                        >  2:51949/1‑249 (MQ=255)                                                                                                            cATTGCCAGCGTTTCCCATACGCCACGAACCAGCAGCCACATCATCGGCTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGAc                                                                                                                                          <  1:158065/250‑1 (MQ=255)                                                                                                                    gCGTTTCCCATACGCCACGAACCAGCAGCCACATCATCGGCTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATGTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGAcc                                                                                                                                 <  1:203385/251‑1 (MQ=255)                                                                                                                             aTACGCCACGAACCAGCAGCCACATCATCGGCTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTc                                                                                                                        >  1:199454/1‑251 (MQ=255)                                                                                                                                       aaCCAGCAGCCACATCATCGGCTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCAGTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAGCTCCAGACgcagc                                                                                                              <  2:172899/251‑1 (MQ=255)                                                                                                                                                            cTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAAt                                                                                         >  1:76627/1‑251 (MQ=255)                                                                                                                                                              cAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAATg                                                                                        <  1:111886/250‑1 (MQ=255)                                                                                                                                                                   aTAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTACTCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAATGGCTCCg                                                                                  >  1:200875/1‑251 (MQ=255)                                                                                                                                                                                           aTGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCAATTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATcgt                                                          <  2:190971/251‑3 (MQ=255)                                                                                                                                                                                                               cAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCCCCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAATGTCTCCGCTTGCAGAAGTTCCTGGTAATCTTCCGGGATATCCAGATGCAgg                                      <  2:62517/251‑1 (MQ=255)                                                                                                                                                                                                                   ggcggcTTGCGTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TACCGAACTTAACGCCACCGGCGTAATCCATATGCGCGCTAATACTGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGATATCCAGATGCAGGGTCg                                  >  1:28068/1‑251 (MQ=255)                                                                                                                                                                                                                             gTATCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAATGGCTCCGCTTTCAGACGTTCCTGGTAATCTTCCGGGATATCCAGATGCAGGGTCGACTGAATaaa                        <  2:126812/251‑1 (MQ=255)                                                                                                                                                                                                                                tCTTGTTGTGTGCCGTGCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCCGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGATATCCAGATGCAGGGTCGACTGAATAAACtt                     >  2:29226/1‑251 (MQ=255)                                                                                                                                                                                                                                                gCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTTGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACg                                                                        >  1:50250/1‑184 (MQ=255)                                                                                                                                                                                                                                                gCATTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAAGGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGGAAATGGCTCCGCTTGCAGACg                                                                        <  2:50250/184‑1 (MQ=255)                                                                                                                                                                                                                                                  aTTTCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGATATCCAGATGCAGGGTCGACTGAATAAACTTCTGCGCCAGCGGCGtttt   <  1:141270/251‑1 (MQ=255)                                                                                                                                                                                                                                                   tttCAATCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGAGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGATATCCAGATGCAGGGTCGACTGAATAAACTTCTGCGCCAGCGGCGttttt  >  1:26360/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCACGAACCAGCAGCCACATCATCGGCTCAGACATAACCCAGTACCTCTACTTTTACATGGTGTTCCTGCAGCCAGGCAATGGCGGCTTGCGTATCTTGTTGTGTGCCGTGCATTTCAGTCAGCATGA‑TGCCGAACTTAACGCCACCGGCGTAATCCATCTGCGCGCTAATAATGTTGTTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGATATCCAGATGCAGGGTCGACTGAATAAACTTCTGCGCCAGCGGCGTTTTC  >  NC_000913/221458‑221950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑