Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,430	C→T	L233L (CTG→TTG)	thrC →	threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,430	0	C	T	100.0%	103.1 / NA	29	L233L (CTG→TTG)	thrC	threonine synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/13);  total (16/13)

GAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGT  >  NC_000913/4186‑4676                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       gAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGc                                                                                                                                                                                                                                                  <  2:7101/251‑1 (MQ=255)      tGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTATTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGAAGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGAAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAgg                                                                                                                                                                                                                                               >  1:181159/1‑249 (MQ=255)                 tATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAAcc                                                                                                                                                                                                                                   >  1:16089/1‑250 (MQ=255)                             aaaaTCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCTCAACCAGCTGGTTGtctc                                                                                                                                                                                                                      <  1:214738/251‑1 (MQ=255)                                     tCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCa                                                                                                                                                                                                               >  1:170515/1‑250 (MQ=255)                                       cACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACTGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGGACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCacgc                                                                                                                                                                                                            >  1:275456/1‑248 (MQ=255)                                              aGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACt                                                                                                                                                                                                     >  2:211284/1‑251 (MQ=255)                                                   aaCTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGc                                                                                                                                                                                                >  1:4654/1‑251 (MQ=255)                                                                  tGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGCAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTCCTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCgggg                                                                                                                                                                                 >  2:94688/1‑250 (MQ=255)                                                                       ggcAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAAGCAGCTGGTTGTCTCGGTGCCAAGCGGAGACTTCGGCGATTTGACGGCTGGTctgct                                                                                                                                                                            <  2:126672/251‑1 (MQ=255)                                                                         cAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGCCGGCGGGTctgtcgg                                                                                                                                                                          >  2:79042/1‑247 (MQ=255)                                                                                gAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCggggcttgtggcggaggc                                                                                                                                                                   >  2:229045/1‑236 (MQ=255)                                                                                            agcGACTGCGATTTCCATGCCGGTCAGGCGCTTGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGAGTTAATCTCGGCTACCTCGATTAACATCATCCGTTTGCTGGCGCAGATTTGCTACTTCTTTGTAGCTGGTGCGCAGTTGCCGCAGGAGGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGTGAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGcc                                                                                                                                                       <  1:193479/249‑1 (MQ=255)                                                                                                  ggCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGAAGCCCCTCGGCCTGCCggggg                                                                                                                                                  >  2:87782/1‑247 (MQ=255)                                                                                                          cGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGttttt                                                                                                                                         <  1:211284/251‑2 (MQ=255)                                                                                                              gCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATtg                                                                                                                                      <  2:189554/250‑1 (MQ=255)                                                                                                                             gTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGaa                                                                                                                      <  1:82725/251‑1 (MQ=255)                                                                                                                                aaGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGATAACTCGATTAGCATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTGAGCCGGCGACGTCACTCGGTCGGGCGGTGAAGAGTTTTTTTGCTGCGATCaaagtagaagc                                                                                                                   >  1:140306/1‑242 (MQ=255)                                                                                                                                 aGCAGTCGTTTGATGATGAAGAACTGAAAGTGTCGGTAGGGTTAAACTCGTCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGAt                                                                                                                  <  2:222027/251‑1 (MQ=255)                                                                                                                                               gatgaAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGtt                                                                                                     <  1:141232/250‑1 (MQ=255)                                                                                                                                                              ggggCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGGCTCGGGGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCGACGTGAACGATGCCGTGCCACGGTTCCTGCACGACGGTCa                                                                                     <  2:125915/249‑1 (MQ=255)                                                                                                                                                                                      aaCTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAACGACTCAg                                                               <  1:54638/249‑1 (MQ=255)                                                                                                                                                                                          cGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGAc                                                          <  1:67490/250‑1 (MQ=255)                                                                                                                                                                                                     aGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGGAATCACCCGGGCTGCCGGTGAAAAGGTTTATTTCTGCGACCAACGTGTACGATAACGCGCCACGGTTCCTGCACGACGGGCAGTGGGCAACCAAAACGACCCAGGGGGCGTTATCCAAcgc                                              >  1:56647/1‑251 (MQ=255)                                                                                                                                                                                                         gTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGGGAAGTCACTCCGTCTGCCGGGGAAAAGTTTTTTTGCTGCGACCAAAGGGGACGAAACCGTGCCACCTTTCCTGCCCGACGGTCCGGGGGCCCCCAAAGCGACCCCGGCGACGGTATTCAAcacgc                                            >  1:17040/1‑245 (MQ=255)                                                                                                                                                                                                                                     tGAAGCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGGCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGGGAACGATACCGTGCCCCGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGGCTCAGGCGACGGTATTCAAAGCGATGGACGTGAGTCAGCCGAACAAATGGcccc              >  1:247288/1‑249 (MQ=255)                                                                                                                                                                                                                                         gCTGTTGCGCAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCCCACCGTCTGCCGGGGGAACGTTTTTTTTTTTCGGACAACCTGAACGATACCGTGCCCCGTTTTCTGCACGCCGGGCAGGGGTCACCCACACCGACCCACGCGCCGGTATCCCACGCGATGGGCGGGAGTCCGCCGCACAAACGGCcgcgcgc          >  2:48633/1‑248 (MQ=255)                                                                                                                                                                                                                                                cgcAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGGTGGCGAAGTCACCCGGGCTGCCGGGGAAAAGATTTATTGCTGCGACCCACGGGGACGATAACGGGCCACGCTTCCTGCACGACGGTCAGGGGGCACCCAAAACGACACAGGCGACGTTATCCCAAGCGATGGACGTGAGGCAACCGAAAAAAAGGCcgcggggggggg     >  2:159495/1‑241 (MQ=255)                                                                                                                                                                                                                                                 gcAGTTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAgt  <  1:79407/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGT  >  NC_000913/4186‑4676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑