Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G96.3% 96.0 / NA 27intergenic (+589/+167)rtcR/glpGDNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (12/14);  minor base T (0/1);  total (12/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAA‑TGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATATCGATAAA‑CAGCGTCG‑AGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAA  >  NC_000913/3560206‑3560670
                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                       
tcttctTTTCCCATCAACGTGTTAGAAACTTTAAG‑TTGGTTGTGAAAAATGAGAAAATGG‑CTGTGATTGAGCAGTGGGGGCGCTGCCGTTCCCGGGGTGTTGCC‑GATAT‑GATAAAACAGCGTCG‑AGCAATTGGGAATTTG‑CTCCGCCACTTGGCGGGCGATGCTCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGCCGGCGCCGCCCCGCCATGATGGc                                                                                                                                                                                                                          <  2:250500/250‑1 (MQ=255)
        tccaatcacggtgtTAGCATCTTTGAGTTTTTGGGTGACAA‑CGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGGCCTCCCGCTCCCGGGGGGGGGCC‑GATACCGAAAAA‑CAGCGTCG‑AGCCTTGGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATc                                                                                                                                                                                                                  <  2:249264/240‑1 (MQ=255)
                     gtAGCAACGTTGAGTTTGTGGGTGACAA‑GGCGCAAATTG‑CTGTGATTGAGCAGTGGGTGCGCTACCGTTTCCGGCGTGGTGCC‑GATATCGATAAA‑CAGCGTCG‑AGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGc                                                                                                                                                                                                    <  1:8712/250‑1 (MQ=255)
                             gTTGAGATTGTTGGTGACAA‑TGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATATCGATAAA‑CAGCGTCG‑AGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAt                                                                                                                                                                                            >  2:239623/1‑251 (MQ=255)
                                gagaTTGTTGGTGACAA‑TGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATATCGATAAA‑CAGCGTCG‑AGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                          >  2:205006/1‑250 (MQ=255)
                                      gttgGTGACAA‑TGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATATCGATAAA‑CAGCGTCG‑AGCCATTGGGGATTTG‑CTCAGCCACTTTGAGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc                                                                                                                                                                                   >  1:34745/1‑251 (MQ=255)
                                                       aaaTTGGCTGTGATTGAGCAGTGTGGGCCCTCCCGCTTCCGG‑GTGGTGCCCTATTTCTATAAA‑CAGCG‑CGTCGCC‑TTGGGGGTTTGGCTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGCCTTTGCGATCGTGCCACGGCGTGTTACCCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg                                                                                                                                                                      <  2:80140/249‑1 (MQ=255)
                                                           tG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATATCGATAAA‑CAGCGTCG‑AGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTTAGGTCGCGGCGAATAGTCTGCGGGCTg                                                                                                                                                               >  2:241014/1‑251 (MQ=255)
                                                                  gATTGAGCAGTGCGTGCGCTCCCGCTTCCGGCGGGGTGCC‑GATATCAATAAA‑CAGCGTCA‑AGCCATTGGGGATTTG‑CTCCGCCGTTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGTTGGCCTTGCGAGCGTGCCACGGCTTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag                                                                                                                                                         <  1:262483/251‑1 (MQ=255)
                                                                  gATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATATCGATAAA‑CAGCGTCG‑AGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                                                                                           >  1:128493/1‑249 (MQ=255)
                                                                    ttGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATATCGATAAA‑CAGCGTCG‑AGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga                                                                                                                                                        >  1:164347/1‑250 (MQ=255)
                                                                         cAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATATCGATAAA‑CAGCGTCG‑AGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTAATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct                                                                                                                                                  >  2:183923/1‑251 (MQ=255)
                                                                               gtgcgCTACCGCTTCCGGCGTGGTGCC‑GATATCGATAAA‑CAGCGTCG‑AGACATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTACGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                                                                                                             >  1:262052/1‑250 (MQ=255)
                                                                                      cccGCTTCCGGGGTGGTGCC‑AATATCGATAAA‑CAGCGTCG‑AGCCTTTGGGGATTTG‑CCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                      <  1:175009/249‑1 (MQ=255)
                                                                                            tCCGGCGTGGTGCC‑GATACCGATAAA‑CAGCGTCG‑AGCCATTGGGGATTTG‑CCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                 <  1:46716/249‑1 (MQ=255)
                                                                                             ccGGCTTGTTGCC‑AATACCGATAAG‑CAGCTGCG‑AGCTATTGGGTTTTTG‑TTCCGCCACTTTGCGGGCGATGCGCTCTTTTCCTTGGGTCTGGGTGGCCTTGCGGTCGTGCCGCGGGGTTTGAACCGACCTGGAAGGCGGCGCGGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGTCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACt                                                                                                                               <  1:20472/250‑1 (MQ=255)
                                                                                                                        cAGCGTGG‑AGCCATTGGGGATTTG‑CCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTGGCGTTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                                                                                                     <  2:181602/251‑1 (MQ=255)
                                                                                                                                     aTTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGt                                                                                         >  1:93046/1‑251 (MQ=255)
                                                                                                                                      ttGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACAGCCATGATGGGGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTTTACCAGTTCTTCGGTACTGACTGAACCCTGCTGTTTAACCATTTCGATAATACCGtt                                                                                        >  1:154888/1‑251 (MQ=255)
                                                                                                                                                tgtcccccctcttagggggCGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGtt                                                                                <  1:64648/234‑1 (MQ=255)
                                                                                                                                                         agtntGCGGGCGAGGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCCCGGCGTGTTAACCGACCGGGAAGGCAGCCCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATATTACCGTGGTGACGTTGTgtttgttt                                                                      <  2:262052/249‑1 (MQ=255)
                                                                                                                                                                     aTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTTTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATc                                                          <  1:239623/251‑1 (MQ=255)
                                                                                                                                                                                    tcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt                                            <  1:205006/250‑1 (MQ=255)
                                                                                                                                                                                           gggTGGCCTTGCGATCGTGCCACGGCGTTTTAACCGAACTGGATGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGTTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACTTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttt                                       <  1:203460/248‑1 (MQ=255)
                                                                                                                                                                                                gCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATc                                                          >  2:144867/1‑224 (MQ=255)
                                                                                                                                                                                                                             ggAACGGGAAGGCAGCTCCGCACCGCCATGATGGCTCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTTCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCAGCGCTaaa  <  2:128493/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 cACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGa                            <  1:56887/205‑1 (MQ=255)
                                                                                                                                                                                                                                                 cACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGa                            >  2:56887/1‑205 (MQ=255)
                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                       
TCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAA‑TGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATATCGATAAA‑CAGCGTCG‑AGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAA  >  NC_000913/3560206‑3560670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑