Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,551,843	C→T	G99D (GGC→GAC)	ypeA ←	putative acetyltransferase YpeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,551,843	0	C	T	96.7%	93.4 / NA	30	G99D (GGC→GAC)	ypeA	putative acetyltransferase YpeA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (13/16);  minor base G (0/1);  total (13/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAA  >  NC_000913/2551602‑2552088                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      gCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCa                                                                                                                                                                                                                                               >  1:199914/1‑250 (MQ=255)         aaTGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCttt                                                                                                                                                                                                                                      <  1:228336/251‑1 (MQ=255)          aTGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCtttt                                                                                                                                                                                                                                     >  1:139211/1‑251 (MQ=255)           tGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCttttt                                                                                                                                                                                                                                    >  1:107531/1‑251 (MQ=255)                                  gcCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCTATCAGCTTTTTCTCCAGCCGATTAAGCAACGCAt                                                                                                                                                                                                             <  1:249077/251‑1 (MQ=255)                                                  caANCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCGTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATcccacgccc                                                                                                                                                                                             <  2:252505/251‑1 (MQ=255)                                                    aGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATcccacgcccac                                                                                                                                                                                           <  2:199914/251‑1 (MQ=255)                                                                       aaCGCAGGAGACCGTGCGCATGATAATGGACAGGATGAAGCACAATACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCGGAATTTTCGGGCAGGCACGAGCAATCAGCTTTGTCGCCAGCCGATTAAGCAACGCGTTAGCAATCGCACGCACACGAAACTCTGGAttcgcgcc                                                                                                                                                                        <  2:113621/251‑8 (MQ=255)                                                                                      gcgcATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCGCCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCTCGCCCGCGAAACTCTGGATGCACGCGAAGATAATAAGCAGa                                                                                                                                                         <  1:248420/251‑1 (MQ=255)                                                                                         catcatAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGAccc                                                                                                                                                      <  2:139211/251‑1 (MQ=255)                                                                                                    aCAGGATGAAACTCAGTACTCGTCATCTTCAATCAAACGCTTACCCAGACTCAGACCGTCGGCGTGTTCATATCCCAGGCGTTCATACATGCCGAGAACCATGTCGTTATCTTCCGGCACAGTGATCTGAAGTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGt                                                                                                                                           <  2:116530/251‑1 (MQ=255)                                                                                                                     aCTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCAc                                                                                                                           <  1:119637/250‑1 (MQ=255)                                                                                                                      ctctTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCg                                                                                                                         >  2:56977/1‑251 (MQ=255)                                                                                                                               ttcaATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGAcc                                                                                                                 >  2:80956/1‑250 (MQ=255)                                                                                                                                tcaatcaaACGCTTACCCAGACTCAGCACTTCTGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCCCGAGCAATCAGCTTTTGCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCa                                                                                                                <  2:215592/250‑1 (MQ=255)                                                                                                                                 caatcaaACGCTTACCCAGACTCAGCACGTCGGCGGGTTCATATCCCAGACCTTCATACATTCCGAGAACCATGTCGTTATCTTCCGGCACATTGATCTTAATTTTCGGGCAGTCACGAGCCATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCCCGCCCACGAAACTCTTGATGCACGCCAAGATAATCAGCAGACCCGCGATTCCCTTCATCACCGCCCATCACCGTTCCGCCCACg                                                                                                              >  1:40844/1‑251 (MQ=255)                                                                                                                                      aaaCGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGa                                                                                                                                                         >  2:102424/1‑203 (MQ=255)                                                                                                                                      aaaCGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGa                                                                                                                                                         <  1:102424/203‑1 (MQ=255)                                                                                                                                            ttACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCACGTCACCGTTTAc                                                                                                   >  1:108465/1‑251 (MQ=255)                                                                                                                                                        aGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCa                                                                                        >  2:80237/1‑250 (MQ=255)                                                                                                                                                               cGGCGTGTTCATATCCCAGGCGTTCATCCATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCATCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAaaacaa                                                                                 <  2:30940/250‑1 (MQ=255)                                                                                                                                                                   gtgtTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGAGCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGa                                                                            <  2:95725/251‑1 (MQ=255)                                                                                                                                                                             cccAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGtt                                                                  <  1:259687/251‑1 (MQ=255)                                                                                                                                                                                aGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCAt                                                               <  2:83326/251‑1 (MQ=255)                                                                                                                                                                                 ggCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACt                                                                              >  1:249333/1‑235 (MQ=255)                                                                                                                                                                                                         agTCGTTATCTTCCGGCACATTGATCTGAAGTTTCGGGCAGTCACGAGCAATCAGCGTTTTCGCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGGCATAACCGCCCATCACCGGTCCGACCACGTCACCGGTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGa                                      <  2:82314/250‑1 (MQ=255)                                                                                                                                                                                                                        gCACATTGATCTGAATTTTCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACgc                       >  1:202581/1‑251 (MQ=255)                                                                                                                                                                                                                                         ttCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACTTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCt       <  1:37536/250‑1 (MQ=255)                                                                                                                                                                                                                                         ttCGGGCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTc      >  2:79614/1‑251 (MQ=255)                                                                                                                                                                                                                                              gCAGTCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGACAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCaa  >  2:36253/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      GCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAGCCGATTAAGCAACGCATTGGCAATCCCACGCCCACGAAACTCTGGATGCACGCCAAGATAATAAGCAGACCCGCGATGCCCGTCATAACCGCCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAA  >  NC_000913/2551602‑2552088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑