Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,699,001	C→T	P81P (CCC→CCT)	yfhH →	putative DNA‑binding transcriptional regulator YfhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,699,001	0	C	T	100.0%	48.7 / NA	17	P81P (CCC→CCT)	yfhH	putative DNA‑binding transcriptional regulator YfhH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/9);  total (8/9)

CTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGTCGGCGAAAAACTGATTAAAGAAAATACTGCCGCTATGTACGCAACGCTAAACGTTAATAGTGAAGAGAAACTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTT  >  NC_000913/2698767‑2699208                                                                                                                                                                                                                                           |                                                                                                                                                                                                                cgtgcgtCGTTTCCGCCAGCGTTACCTGGGTCTTGCCCAGAGTGATAAAAAACTGTCGGAGTCTCTGCTGCTACAACCTGATACGGCGCGCTATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCTTGAAGTTCGCGCAAAAACGCGGCTAGAAAGGTTTTCCGGCGACTAAGTTGGCGTTTAGTTAAGCGCTGGCAAGCCAGCCGGAATCACCTTCCGTGCCAATGCATa                                                                                                                                                                                                 <  2:201713/245‑1 (MQ=255)      ttCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCTTCCGTGCCCATTCATAACCa                                                                                                                                                                                             >  1:180530/1‑250 (MQ=255)                             gcgCTTGCCAAGAGTGATAAAAAACTGGCGGATTATCTCCTGCTACACCATGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCAGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCAGGAATAACCTTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACg                                                                                                                                                                     >  2:11261/3‑251 (MQ=255)                              agCTTGCCCCGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACAGCTCGCCATTTAAGCTCTCAGCCACTGGCCAACTACCCCGGAGTCAGTCAGTACAGCGTCGTGAAGTTCGCGCAATCACTCGGCTCTAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCACCCGGAATCACCTTCCGAGCCCATTCATAACCAAATCCCCGGTGCTGATCCGTTACgg                                                                                                                                                                    >  2:94710/2‑251 (MQ=255)                                            gATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCCCCTTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGTCGGCGaaaa                                                                                                                                                      <  2:237439/251‑1 (MQ=255)                                                                   gctgctACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCTTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGTCGGCGAAAAACTGATTAAAGAAAATACTGc                                                                                                                                 <  2:123620/249‑1 (MQ=255)                                                                         aCAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTTAAGCGCTGGCAAGCCAGCCGGAATCACCTTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGTCGGCGAAAAACTGATTAAAGAAAATACTGCCGCTATg                                                                                                                          <  1:20069/250‑1 (MQ=255)                                                                                                   gCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTAGTGGCGTTCGAGAAAAAACTCGGCTATAAAGCTTTTCCCGCGCTTAAGTTGACGTTGAATGAAGCGCTGGCAAGCAAGCAGGAATCACCTTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATACGTTACGGCTGGTAGGAGAAAACCTGCTTACAGAAAATACTGCCGCTATGTACGCAACGCTAACCGTTAATAGTGa                                                                                                >  1:40640/1‑250 (MQ=255)                                                                                                                ggCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCTTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGTCGGCGAAAAACTGATTAAAGAAAATACTGCCGCTATGTACGCAACGCTAAACGTTAATAGTGAAGAGAAACTGCATg                                                                                  <  1:24643/251‑1 (MQ=255)                                                                                                                                gtcagtcagtcCAGCGTCGTGAAGTTCGCGCAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCTTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGTCGGCGAAAAACTGATTAAAGAAAATACTGCCGCTATGTACGCAACGCTAAACGTTAAGAGTGAAGAGAAACTGCATGAATGCGTAACAATGtt                                                                  <  2:180530/251‑1 (MQ=255)                                                                                                                                              cgtcgtGAAGTTCGCGCAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCTTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGTCGGCGAAAAACTGATTAAAGAAAATACTGCCGCTATGTACGCAACGCTAAACGTTAATAGTGAAGAGAAACTGCATGAATGCGTAACAATGTTGCGCTCTGCgcggc                                                    <  1:39283/251‑1 (MQ=255)                                                                                                                                                                          tataAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCTTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGTCGGCGAAAAACTGATTAAAGAAAATACTGCCGCTATGTACGCAACGCTAAACGTTAATAGTGAAGAGAAACTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTc                        >  2:229110/1‑251 (MQ=255)                                                                                                                                                                             aaaGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCTTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGTCGGCGAAAAACTGATTAAAGAAAATACTGCCGCTATGTACGCAACGCTAAACGTTAATAGTGAAGAGAAACTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCggg                     <  2:79992/251‑1 (MQ=255)                                                                                                                                                                              aagtatttCCGCCGCTTAAGTTGGCGCTCAGTGAAGTTCTGGCAAGCCTGCCGGACTCACCTTCCGTGCCCATTCATAACCACATCCGCGGTGATGATCCGTTACGGCTGGTCGGCGAAAAACTGTTTAAAGAAAAAACTCCCGCTATGTACGCAACGCTCACAGCTAATAGTGAAGAGAAACTGCATGTATGCGGAACAATGTTGCGCCCTGCGCGGCGGATTATTCTGACCGGTATAGGCGCTTCGGGt                    >  2:26902/6‑251 (MQ=255)                                                                                                                                                                                              tAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCTTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGTCGGCAAAAAACTGATTAAAGAAAAAACTGCCGCTATGTACGCAACGCTAAACGTTAATAGTGAAGAGAAACTGCATGAATGCGTAACAATGGTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACt    >  1:285728/1‑251 (MQ=255)                                                                                                                                                                                                aggtGGCGTTGAGTGAAGCGCTGGCAAGCCAGCAGGAATCACCTTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGTCGGCGAAAAACTGATTAAAGAAAATACTGCCGCTATGTACGCAACGCTAAACGTTAATAGTGAAGAGAAACTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCTCAAAACttt  <  2:153134/248‑1 (MQ=255)                                                                                                                                                                                                aGTTGGCGTTGAGTGAACCGCTGGCAAGCCAGCCGGAATCACCTTCCGCGCCCATTCATAACCAAATCCGCGGTGATGCCCCGTTACGACTGGTCGGCGCCAAACCGATTAAAGAAAATACTGCCGCTATGTACGCAACGCTAAACGTTAATAGTGAAGAGAAACTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGCCCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACttt  >  2:277110/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                CTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGTCGGCGAAAAACTGATTAAAGAAAATACTGCCGCTATGTACGCAACGCTAAACGTTAATAGTGAAGAGAAACTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTT  >  NC_000913/2698767‑2699208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑