Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,660,164	C→T	K42K (AAG→AAA)	iscU ←	scaffold protein for iron‑sulfur cluster assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,660,164	0	C	T	100.0%	110.2 / NA	33	K42K (AAG→AAA)	iscU	scaffold protein for iron‑sulfur cluster assembly
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/18);  total (15/18)

TTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTCAGATCCACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTT  >  NC_000913/2659918‑2660409                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       tttGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTtct                                                                                                                                                                                                                                                    >  1:67585/1‑250 (MQ=255)               ccGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCcaca                                                                                                                                                                                                                                       <  1:71688/250‑1 (MQ=255)                 gCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGc                                                                                                                                                                                                                                    >  1:148562/1‑251 (MQ=255)                        cggcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGcc                                                                                                                                                                                                                             >  1:142691/1‑251 (MQ=255)                           cTTTGCTCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGACAGTTCAAGGTGTTCCGCAATATCGGAGTTTTTGATCGCCTGCGCTTAGTCGCGGGACTTCCATTTCCCCCATTCGGGGCACC‑GGGGGCTGGGAGCGATAGCGGAACCGCAGCCGTAAGCTTTAAAACGCGCGTCTTCAATGCTACGTGCGTCGTTGACTTTAATCTGCACTTTCCTCGCGTAGCCGGAGGCCGGTGacccc                                                                                                                                                                                                                          >  1:277977/1‑251 (MQ=255)                             ttGATCGCGTCTTCTGCCAGAATAGAACAGTGAAGTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGccccc                                                                                                                                                                                                                          <  1:144302/249‑1 (MQ=255)                                                     gAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACTGGCCGGTTCCCCCACCATGCCGCTGCCGACGTTCtcgtc                                                                                                                                                                                                <  1:231770/251‑1 (MQ=255)                                                             aagtttCACCGTCGGCAGTTCAAGTTGTTCAGCAATATCGGTGTTTTTGATCGCCTGCTCTTCGTCGAGAGACTTCCCTTTCGCCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCATCCGTAAGTTTTAAAACCCGCGTCTTCAATGATACCTTCATCGTTGCCTTTAATCTGCAATTTCAGCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTtgttgt                                                                                                                                                                                        <  2:63671/248‑1 (MQ=255)                                                                        gcggcAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCGTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAAccc                                                                                                                                                                             <  1:281068/251‑1 (MQ=255)                                                                          ggcAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCTGTAAGTTTTAAAACGCGCGTCTGCAATTATACCTTCAGCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGGTGTCAAAGGAACCCAc                                                                                                                                                                           <  2:135628/251‑1 (MQ=255)                                                                                tCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTAc                                                                                                                                                                      <  1:208036/250‑1 (MQ=255)                                                                                   aGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCgg                                                                                                                                                                  >  1:207528/1‑251 (MQ=255)                                                                                         tcAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGAtt                                                                                                                                                               <  1:193854/248‑1 (MQ=255)                                                                                         tcAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATtctc                                                                                                                                                            >  1:70485/1‑251 (MQ=255)                                                                                                       gtTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGGG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCACACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGACGATa                                                                                                                                              >  1:122946/1‑250 (MQ=255)                                                                                                                     gcgcTTCGGCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCAGCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGt                                                                                                                                 <  1:147259/249‑1 (MQ=255)                                                                                                                              cgAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATtata                                                                                                                      <  1:241440/251‑1 (MQ=255)                                                                                                                                 gagaCTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAAt                                                                                                                   <  1:190535/251‑1 (MQ=255)                                                                                                                                    aCTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATtct                                                                                                                >  1:103042/1‑251 (MQ=255)                                                                                                                                        cccTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCt                                                                                                             <  1:53551/250‑1 (MQ=255)                                                                                                                                         ccTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGa                                                                                                           <  2:84247/251‑1 (MQ=255)                                                                                                                                               aCCCATTCGGTG‑ACCAGGGAGCTGGAAGCAATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCg                                                                                                      >  2:145461/1‑250 (MQ=255)                                                                                                                                                                           gCGATAGTGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCAGTCGAt                                                                          <  2:18531/251‑1 (MQ=255)                                                                                                                                                                                    gAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTc                                                                   >  1:265325/1‑249 (MQ=255)                                                                                                                                                                                    gAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTCAg                                                                 <  2:103042/251‑1 (MQ=255)                                                                                                                                                                                             ccGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTCAGATCCACGcc                                                        >  2:231536/1‑251 (MQ=255)                                                                                                                                                                                                      ttAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCCGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACTCGGATTCTCGCAA‑TGGTCGATAACTTTTTCGTTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTCAGATCAACGCCCTGCTTGt                                                >  1:211668/1‑250 (MQ=255)                                                                                                                                                                                                         aaaCGCGCGACTTCAATGAGAAATTCATCGTTGGCTTTAATATGCAATTACCTCACGACGCTACAGGTGGGAGCCCCAACCATGCCGCTGCAGAAGTTCACGTCGGTGTTGTCAAAGGGACCCCAGTGACGCGGATTCT‑GTAAGTGGACGATAAATTTTTCGCTGTAAGCCATTATAAATTCTCCTGAATCCGATACAGATTAATGATGAGCCCATTCAATACTGTTCAGATCCACGCACTGCTTGTACAt                                            >  2:180054/1‑251 (MQ=255)                                                                                                                                                                                                                  tCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTATCCTGATTACGATACCGTTTAATGATGAGCCCATTCGATGCTGTTCAGATCCACGCCCTGCTTGTACATTTCCcac                                     >  2:3271/1‑249 (MQ=255)                                                                                                                                                                                                                    ttCAATGATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTCAGATCCACGCCCTGCTTGTACATTTCCCACAGc                                  <  2:127216/250‑1 (MQ=255)                                                                                                                                                                                                                          gATACCTTCATCGTTGACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTCAGATCCACGCCCTGCTTGTACATTTCCCACAGCGGAGaa                            >  1:84705/1‑250 (MQ=255)                                                                                                                                                                                                                                         gACTTTAATCTGCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATTAGCCCATTCGATGCTGTTCAGATCCACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAgacgac            <  1:265415/251‑1 (MQ=255)                                                                                                                                                                                                                                                    gCAATTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTCAGATCCACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGAttt  <  1:202114/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       TTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTCCCTTTCACCCATTCGGTG‑ACCAGGGAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAA‑TGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTCAGATCCACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTT  >  NC_000913/2659918‑2660409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑