Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 556,203 C→T W117* (TGG→TGA)  ybcI ← PF04307 family inner membrane protein YbcI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,2030CT95.7% 63.7 / ‑5.1 24W117* (TGG→TGA) ybcIPF04307 family inner membrane protein YbcI
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (9/13);  minor base G (0/1);  total (9/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

CGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATT  >  NC_000913/555968‑556397
                                                                                                                                                                                                                                            |                                                                                                                                                                                                  
cGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACAGTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCGCCTGCCAGGGATCTAAAAAACGTTCAT‑CTGACCACGGTCACAGCCAGCCAACg                                                                                                                                                                                     <  1:17618/251‑1 (MQ=255)
     gggACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCttt                                                                                                                                                                                <  1:137094/251‑1 (MQ=255)
         ccaccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCg                                                                                                                                                                            <  1:152006/251‑1 (MQ=255)
           cccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTACCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGcc                                                                                                                                                                          <  2:85817/250‑1 (MQ=255)
                  gtcgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGtt                                                                                                                                                                   >  2:49334/1‑251 (MQ=255)
                                       gCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCCCCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGc                                                                                                                                              <  1:49334/251‑1 (MQ=255)
                                           gataggggTTTTCGCATCCGGTTACCGTCTGCGCCACCACAACATTCCATTCTGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGAGGCCCGTACGGCGGGGTTGAACGAGACAATGCAAACGGGGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCTTTCAT‑CTGACCAGGGTCCCAGCCAGCAAACGCTTTTGCCGCCAGTGGTTACCGAATCCATCAAGCTGTGCGATa                                                                                                                                          <  2:274121/244‑1 (MQ=255)
                                                     ttCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGAc                                                                                                                                >  1:73817/1‑251 (MQ=255)
                                                              ggTTATCGTCTGCGCCACCACACCATGCCCATCAAGAGCATCTCCGGCACCCACCCCCACATCAATTCAGAAAGAATCCCCTGATGCCCTTACGGTGGGGTGTAACGAGACAATTCAAACGGCCCGCCTGTTATCCCCTGCGAGGGAGAGAAAAAGC‑TTTATGCGGCCCACGGTCTCAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTaaaaa                                                                                                                       <  2:283350/251‑1 (MQ=255)
                                                               gTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAc                                                                                                                      >  1:198952/1‑251 (MQ=255)
                                                                         gcgcCACCACAACATCCCCACCGGGCGCATCTCCGGCAACCACTCCCACATCAATTAAGAAATAACCCCCTATTGCCCGGACGGCGTGGTGTAACGAGCAAATGCAAGCGGCGCGACTTTTTTCACCTGCCAGGGAGCGAAAAAGCGTACAT‑CTGACCACGTTCACAGCCAGCCACCGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCagcagc                                                                                                            <  2:100738/251‑1 (MQ=255)
                                                                           gcCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAACTAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCg                                                                                                           >  1:159701/1‑250 (MQ=255)
                                                                                              cAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAAc                                                                                        <  1:176766/250‑1 (MQ=255)
                                                                                                    catctgcggtaaCCACACTCACATCAATCCAGAAATAATCACCTGATGCCCGTACGGCGTGGAGTAACGAGACAATGCAAAAGGAGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTAGCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCCACGAGACGGTTAAAAACAGCCAGCAGCGAATAAGCCCAGCCCTGAACCAtacgc                                                                                 >  2:122613/11‑247 (MQ=255)
                                                                                                                                  aGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGaca                                                    >  2:70225/1‑250 (MQ=255)
                                                                                                                                         atCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGc                                            >  2:49024/1‑251 (MQ=255)
                                                                                                                                            cccTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCaaa                                         <  1:70225/250‑1 (MQ=255)
                                                                                                                                              cTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAAc                                                                                        >  1:236973/1‑202 (MQ=255)
                                                                                                                                                gATGCCGGTACGGCTTGTTGTAACGAGCCAATGAAAACGGCGCGCCTTTTACCACCTGCCAGAGAGCGAAAAAGCTTTCAC‑CTGACCACGGGCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAacac                                      <  1:215467/250‑1 (MQ=255)
                                                                                                                                                            gCGTGGGGTAGCGAGACAATGCAAACGGCGCGACTTTTATCCCCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCGGCCAAGGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa                         <  1:133899/251‑1 (MQ=255)
                                                                                                                                                                        gagaCAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATg              >  2:206201/1‑250 (MQ=255)
                                                                                                                                                                          aaaaaTGCAAACGGCGCGACTTTAATCCCCTGCCGGGGAGCGAAAAGTCGTGCAT‑CTGACCACGGTCACACCCAGCCAACGCTTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGAcc           <  2:202003/248‑1 (MQ=255)
                                                                                                                                                                                  aaaCGGCGCTTCTTTTATCCCCTGACAGGGAGCGAAAAAGCGTTCAT‑CTGCCCACGGCCCCAGCAAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGTTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGTGTAAACCCGCGATGACCAAAAACAt   <  1:265229/251‑1 (MQ=255)
                                                                                                                                                                                   aaCGGCGCGACTTTTATCACCTGCCGGGGAGCGAAAAAGCGTTCAT‑CTGACCCCGGTCACAGCCAGCCACCGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACAtt  <  1:49024/251‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                  
CGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATT  >  NC_000913/555968‑556397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑