Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,063,945	C→T	W117* (TGG→TGA)	ompL ←	putative outer membrane porin L

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,063,945	0	C	T	100.0%	50.9 / NA	16	W117* (TGG→TGA)	ompL	putative outer membrane porin L
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/5);  total (11/5)

GTGTTAGTGATTTCCCAATGATGATCTTTCCCGTTGCTACTATTAAAGTCATTCACTCGCATGAAGTAATGTGGCTCAAATGTATAGGAAAATTTATCGGTGATTTTAAAATTCCAGTAGGTGCCAATTTCATAGGTGTCATTATTATCCAGTTCCCCGCTCAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAACCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCACCACCCGAACCGATACTCTTATCATTAATTAAGCCACCGGGCTGGATGGTTAATTTATCGGTTGGTTTAAATAACGGATACCAGCCTTCAATTTCGTTATATCCATGTTTTAGTTCATCTTCTCGCTGAAAGTTGTAGGTATTGGTTAACATAATACCCGCGCCCATATCGAAGTTATAACCCACACGTAGCATAACCTCG  >  NC_000913/4063727‑4064185                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 gggtTAGTGATTTCCCAATGATGATCTTTCCCGTTGCTACTATTAAAGTCATTCACTCGCATGAAGTAATGTGGCACAAATGTATAGGAAAATTTATCGGTGATTTTAAAATTCCAGTAGGTGCCAATTTCATAGGTGTCATTATTATCCAGTTCCCCGCTCAAATCTGTTCAACTATAGTTATTAGGGTTATAGCGATTTCGAACTGTCAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGAt                                                                                                                                                                                                                   >  2:44354/3‑250 (MQ=255)                            ttCCCGTTGCTACTATTAAAGTCATTCACTCGCATGAAGTAATGTGGCTCAAATGTATAGGAAAATTTATCGGTGATTTTAAAATTCCAGTAGGTGCCAATTTCATAGGTGTCATTATTATCCAGTTCCCCGCTCAAATCTGTTTAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGATACACGGCTCCACCCGAACCGATActct                                                                                                                                                                                        <  2:173854/250‑1 (MQ=255)                                  ttGCTACTATTAAAGTCATTCACTCGCATGAAGTAATGTGGCTCAAATGTATAGGAAAATTTATCGGTGATTTTAAAATTCCAGTAGGTGCCAATTTCATAGGTGTCATTATTATCCAGTTCCCCGCTCAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCACCACCCGAACCGATACTCTTATCatt                                                                                                                                                                                 >  2:244126/1‑251 (MQ=255)                                  ttGCTACTATTAAAGTCATTCACTCGCATGAAGTAATGTGGCTCAAATGTATAGGAAAATTTATCGGTGATTTTAAAATTCCAGTAGGTGCCAATTTCATAGGTGTCATTATTATCCAGTTCCCCGCTCAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTAGTACTGTCAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCACCACCCGAACCGATACTCTTATCatt                                                                                                                                                                                 >  1:198476/1‑251 (MQ=255)                                                      cACTCGCATGAAGTAATGTGGCTCAAATGTATAGGAAAATTTATCGGTGATTTTAAAATTCCAGTAGGTGCCAATTTCATAGGTGTCATTATTATCCAGTTACCCGCTCAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAATCATGGTACAACTTTATAGTTGACGTCCAGATACACTGCACCACCCGAACCGATACTCTTATCATTAATTAAGCCACCGGGCTgg                                                                                                                                                              >  1:202317/1‑250 (MQ=255)                                                               gAAGTAATGTGGCTCAAATGTATAGGAAAATTTATCGGTGATTTTAAAATTCCAGTAGGTGCCAATTTCATAGGTGTCATTATTATCCAGTTCCCCGCTCAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCGCCACCCGAACCGATACTCTTATCATTAATTAAGCCACCGTGCTGGATGGTTAAtt                                                                                                                                                    <  1:219703/251‑1 (MQ=255)                                                                                       ggAAAATTTATCGGTGATTTTAAAATTCCAGTAGGTGCCAATTTCATAGGTGTCATTATTATCCAGTTCCCCGCTCAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCACCACCCGAACCGATACTCTTATCATTAATTAAGCCACCGGGCTGGATGGTTAATTTATCGGTTGGTTTAAATAACGGAt                                                                                                                            <  1:62617/251‑1 (MQ=255)                                                                                        gAAAATTTATCGGTGATTTTAAAATTCCAGTAGGTGCCAATTTCATAGGTGTCATTATTATCCAGTTCCCCGCTCAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCACCACCCGAACCGATACTCTTATCat                                                                                                                                                                                  <  1:283251/196‑1 (MQ=255)                                                                                        gAAAATTTATCGGTGATTTTAAAATTCCAGTAGGTGCCAATTTCATAGGTGTCATTATTATCCAGTTCCCCGCTCAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCACCACCCGAACCGATACTCTTATCat                                                                                                                                                                                  >  2:283251/1‑196 (MQ=255)                                                                                                 tCGGTGATTTTAAAATTCCAGTAGGTGCCAATTTCATAGGTGTCATTATTATCCAGTTCCCCGCTCAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCACCACCCGAACCGATACTCTTATCATTAATTAAGCCACCGGGCTGGATGGTTAATTTATCGGTTGGTTTAAATAACGGATACCAGCCTTc                                                                                                                  >  2:263253/1‑251 (MQ=255)                                                                                                    gTGATTTTAAAATTCCAGTAGGTGCCAATTTCATAGGTGTCATTATTATCCAGTTCCCCGCTCAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCACAACCCGAACCGATACTCTTATCATTAATTAAGCCACCGGGCTGGATGGTTAATTTATCGGTTGGTTTAAATAACGGATACCAGCCTTCAAt                                                                                                               >  2:275819/1‑251 (MQ=255)                                                                                                                                                 ttatCCAGTTCCCCGCTAAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAATCATGGTACAACTTTATAGTTGCCGTCCAGATACACTGCACCACCCGAACCGATACTCCTATCATGAAAAAAGCCACCGGGCTGGCTGGTTAATTTATCGGTTGGTTGAAATAACGGATACCAGCCTTCAATTTCGTTATATCCATGTTTTAGTTCATCTTCTCGCTGAAAGTTGt                                                                   >  2:275836/1‑250 (MQ=255)                                                                                                                                                            cccGCTCAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCACCACCCGAACCGATACTCTTATCATTAATTAAGCCACCGGGCTGGATGGTTAATTTATCGGTTGGTTTAAATAACGGATACCAGCCTTCAATTTCGTTATATCCATGTTTTAGTTCATCTTCTCGCTGACAGTTGTAGGTATTGGtt                                                        >  1:66542/1‑250 (MQ=255)                                                                                                                                                                 tCAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCACCACCCGAACCGATACTCTTATCATTAATTAAGCCACCGGGCTGGATGGTTAATTTATCGGTTGGTTTAAATAACGGATACCAGCCTTCAATTTCGTTATATCCATGTTTTAGTTCATCTTCTCGCTGAAAGTTGTAGGTATTGGTTAACat                                                   >  1:186919/1‑250 (MQ=255)                                                                                                                                                                               cTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCACCACCCGAACCGATACTCTTATCATTAATTAAGCCACCGGGCTGGATGGTTAATTTATCGGTTGGTTTAAATAACGGATACCAGCCTTCAATTTCGTTATATCCATGTTTTAGTTCATCTTCTCGCTGAAAGTTGTAGGTATTGGTTAACATAATACCCGCGCCCat                                    >  1:17953/1‑251 (MQ=255)                                                                                                                                                                                                                  cAGATTAAATCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCACCACCCGAACCGATTCTCTTATCATTAATTAAGCCACCGGGCTGGATGGTTAATTTATCGGTTGGTTTAAATAACGGATACCAGCCTTCAATTTCGTTATATCCATGTTTTAGTTCATCTTCTCGCTGAAAGTTGTAGGTATTGGTTAACATAATACCCGCGCCCATATCGAAGTTATAACCCACACGTAGCATAACCTCg  <  1:68033/250‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 GTGTTAGTGATTTCCCAATGATGATCTTTCCCGTTGCTACTATTAAAGTCATTCACTCGCATGAAGTAATGTGGCTCAAATGTATAGGAAAATTTATCGGTGATTTTAAAATTCCAGTAGGTGCCAATTTCATAGGTGTCATTATTATCCAGTTCCCCGCTCAAATCTGTTGAACTATAGTTATTATGGTTATAGCGATTTCGTACTGTCAGATTAAACCATGGTACAAATTTATAGTTGACGTCCAGATACACTGCACCACCCGAACCGATACTCTTATCATTAATTAAGCCACCGGGCTGGATGGTTAATTTATCGGTTGGTTTAAATAACGGATACCAGCCTTCAATTTCGTTATATCCATGTTTTAGTTCATCTTCTCGCTGAAAGTTGTAGGTATTGGTTAACATAATACCCGCGCCCATATCGAAGTTATAACCCACACGTAGCATAACCTCG  >  NC_000913/4063727‑4064185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑