Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,267,582	G→A	G157S (GGC→AGC)	tyrB →	tyrosine aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,267,582	0	G	A	100.0%	62.9 / NA	19	G157S (GGC→AGC)	tyrB	tyrosine aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/12);  total (7/12)

ATCGCCATGCCATTGCGCCGCTGCTGTTTGGTGCGGACCATCCGGTACTGAAACAACAGCGCGTAGCAACCATTCAAACCCTTGGCGGCTCCGGGGCATTGAAAGTGGGCGCGGATTTCCTGAAACGCTACTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTG  >  NC_000913/4267337‑4267819                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              aTCGCCATGCCATTGCGCCGCTGCTGTTTGGTGCGGTCCATCCGGTACTGAAACAACAGCGCGTAGCAACCATTCAAACCCTTGGCGGCTCCGGGGCATTGAAAGTGGGCGCGGATTTCCTGAAACGCTACTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGGTGCGACTAACAGCGt                                                                                                                                                                                                                                           <  2:166098/250‑1 (MQ=255) aTCGCCATGCCATTGCGCCGCTGCTGTTTGGTGCGGACCATCCGGTACTGAAACAACAGCGCGTAGCAACCATTCAAACCCTTGGCGGCTCCGGGGCATTGAAAGTGGGCGCGGATTTCCTGAAACGCTACTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGTCTAACAGCg                                                                                                                                                                                                                                            <  2:281630/249‑1 (MQ=255) aTCGCCATGCCATTGCGCCGCTGCTGTTTGGTGCGGACCATCCGGTACTGAAACAACAGCGCGTAGCAACCATTCAAACCCTTGGCGGCTCCGGGGCATTGAAAGTGGGCGCGGATTTCCTGAAACGCTACTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACAGCGTg                                                                                                                                                                                                                                          <  2:65315/251‑1 (MQ=255)    gccatgccatTGCGCCGCTGATGTTTGGTGCGGACCATCCGGTACTGAAACAACAGCGCGTAGCAACCATTCAAACCCTCGGCGGCTCCGGGGCATTGAAAGTGGGCGCGGATTTCCTGAAACGCTACTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACAGCGTgcgc                                                                                                                                                                                                                                       >  1:210180/1‑251 (MQ=255)               gcgcAGCTGCTGTTTGGTGCGGACCATCCGGTACTGAAACAACAGCGCGTAGCAACCATTCAAACCCTTGGCGGCTCCGGGGCATTGAAAGTGGGCGCGGATTTCCTGAAACGCTACTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTTCCCCTGGTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCt                                                                                                                                                                                                                            <  2:208913/251‑1 (MQ=255)                                                    aacaacAGCGCGTAGCAACCATTCAAACCCTTGGCGGCTCCGGGGCATTGAAAGTGGGCGCGGATTTCCTGAAACGCTACTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTAtt                                                                                                                                                                                       <  2:188220/251‑1 (MQ=255)                                                                          tcaaaacgtggaaggcTCCGGGGCATTGAAAGTGGGCGCGGCTTACCTGAAACGCTACTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACATGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTAATTACCCCTGGTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAAAATTAACTACCCGCAGTATTGTGTTGCTGCATCCATGTTGcc                                                                                                                                                                 >  2:12161/14‑251 (MQ=255)                                                                                                              cgcgGATTTCCTGAAACGCTACTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTTCCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACAGTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGt                                                                                                                             <  1:285527/251‑1 (MQ=255)                                                                                                                cgGATTTCCTGAAACGCTACTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTgg                                                                                                                           <  1:46416/251‑1 (MQ=255)                                                                                                                       ccNGAAACGCTACTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCATCGGGTGCCGATCTCACTAATGATCAGTGGGATGCg                                                                                                                     <  2:259592/250‑1 (MQ=255)                                                                                                                        cTGAAACGCTACTTCACGGAAACAGGCGTCTGGGTCAGTGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGATGGATTCGAAGAGAGTACTTACCCATGGTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGACCGCAGACTTGTGTTGCAGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGGGGGaagtggt                                                                                                                   >  2:251576/1‑245 (MQ=255)                                                                                                                                  aCTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGaaagt                                                                                                         <  2:246483/251‑3 (MQ=255)                                                                                                                                                     cTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTgggct                                                                                                                        >  2:148568/1‑215 (MQ=255)                                                                                                                                                     cTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGAt                                                                                                                        <  1:148568/217‑1 (MQ=255)                                                                                                                                                                     aCCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGatat                                                                      >  1:283026/1‑251 (MQ=255)                                                                                                                                                                                   cGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGAt                                                        >  1:30835/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTc                                                                           <  1:41458/185‑1 (MQ=255)                                                                                                                                                                                                                                  ggTATGACGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTc                                                                           >  2:41458/1‑185 (MQ=255)                                                                                                                                                                                                                                         cGAAGCGACTAACAGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTg  <  2:30835/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              ATCGCCATGCCATTGCGCCGCTGCTGTTTGGTGCGGACCATCCGGTACTGAAACAACAGCGCGTAGCAACCATTCAAACCCTTGGCGGCTCCGGGGCATTGAAAGTGGGCGCGGATTTCCTGAAACGCTACTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTG  >  NC_000913/4267337‑4267819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑