Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	280,533	G→A	S68F (TCC→TTC)	yagB ←	orphan antitoxin YagB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	280,533	0	G	A	100.0%	70.7 / NA	21	S68F (TCC→TTC)	yagB	orphan antitoxin YagB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/13);  total (8/13)

CGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCAC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCCCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATG  >  NC_000913/280299‑280774                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   cGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCTc                                                                                                                                                                                                                                    >  1:71547/1‑251 (MQ=255)                  ttGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAACTTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACATTGAGCGTACCTGGGATTCAGCTCACAGAAAAGCAACATCAGCTCCAGATGGGCG‑Caaaaat                                                                                                                                                                                                                  >  1:189662/1‑249 (MQ=255)                                       tctTCGGGATAACGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAg                                                                                                                                                                                                                                       >  2:39952/1‑210 (MQ=255)                                       tctTCGGGATAACGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAg                                                                                                                                                                                                                                       <  1:39952/210‑1 (MQ=255)                                         ttCGGGATAACGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGt                                                                                                                                                                                            >  1:148621/1‑251 (MQ=255)                                               aTAACGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTc                                                                                                                                                                                      <  1:47384/251‑1 (MQ=255)                                                 aaCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGCGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTg                                                                                                                                                                                    >  2:12509/1‑251 (MQ=255)                                                 aaCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTGTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCTTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCGCCAGATGTGCGACAAAATGCGGGAAGGCTGCATCCAGTTGTAGAGTCt                                                                                                                                                                                     <  2:114798/250‑1 (MQ=255)                                                                                                               aaaCCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCAC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCtt                                                                                                                     <  1:12509/251‑1 (MQ=255)                                                                                                                             tCAAAATTGCGATATATACATACCCATGACTGCCCCGCGTCTCGGCTTCACCAGTCATCCCGTTACGGTCCAGAGATAAACAGTGCGCGTACATGGGATTCAGCTACCAGAAGAGCAGCATCAGCTCCAGATGAGCGACAAAATGCGGGAAGGCTTAATCCAGTTGTAGAATCTG‑ACACTCACTGAATGAGCCGGTGAATCCATACCGGTCAGCCAGACAATGAAGACGATGGCCTTCCAGCACCattc                                                                                                         >  1:109692/1‑246 (MQ=255)                                                                                                                             tCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCAC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCgg                                                                                                       >  2:149110/1‑250 (MQ=255)                                                                                                                                              acatacCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCAC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGgtg                                                                                       <  1:149110/250‑1 (MQ=255)                                                                                                                                                      aTGACGGCCCAGCTTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCTC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATAtctc                                                                              <  1:176787/251‑1 (MQ=255)                                                                                                                                                      aTGAATGCCCAGCGTATCGGCTTCCCCAGTCAGCCCGTTTCGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCAC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATAtctc                                                                              <  2:217787/251‑1 (MQ=255)                                                                                                                                                            gCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCAC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTaa                                                                        <  2:62885/251‑1 (MQ=255)                                                                                                                                                                              aCCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCAC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCCCAAGTTTGAGt                                                        >  1:194338/1‑249 (MQ=255)                                                                                                                                                                                     aGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCAC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAGGCCCCCAAGTTTGAGTAGAGTCttt                                               <  2:83863/251‑1 (MQ=255)                                                                                                                                                                                            tACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCAC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCCCAAGTTTGAGTAGAGTCTTTATTCAt                                         <  2:244262/250‑1 (MQ=255)                                                                                                                                                                                                                           gggATTCAGCGCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAAGGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCAC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCCCAAGTTTGAGTAGAGTCTGTATTCATAATTTTATTCCTGTTAAAGAAGTTCATGTTc          <  2:71547/250‑1 (MQ=255)                                                                                                                                                                                                                                  aGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCAC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCCCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATg  <  1:55780/251‑1 (MQ=255)                                                                                                                                                                                                                                  aGCTCACAGAAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCAC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCCCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATg  <  1:54774/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   CGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCAC‑CTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCCCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATG  >  NC_000913/280299‑280774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑