Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,969,001	C→T	S362S (AGC→AGT)	rfe →	UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,969,001	0	C	T	100.0%	101.6 / NA	34	S362S (AGC→AGT)	rfe	UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/16);  total (18/16)

CCATTTGATCATGCGTGCCGGGTTTACTTCCCGTCAGGCGTTTGTGCTGATTACCCTTGCCGCAGCACTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTCAGGAGTGGAGCTCGACGGCGATTACCGATCGTCCAACGGTGAATATGCTGGGGGGATAT  >  NC_000913/3968758‑3969246                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      ccATTTGATCATGCGTGCCGGGTTTACTTCCCGTTAGGCGTTTGTGCTGATTACCCTTGCCGCAGCCCTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTTTCATTTTGTCCCGGAGTGGGGCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGGAGTCCCAAt                                                                                                                                                                                                                                                 <  2:90736/250‑1 (MQ=255) ccATTTGATCATGCGTGCCGGGTTTACTTCCCGTCAGGCGTTTGTGCTGATTACCCTTGCCGCAGCACTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCGCGCTTTATTAAGCTCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAAtt                                                                                                                                                                                                                                                <  1:120815/251‑1 (MQ=255)  cATTTGATCATGCGTGCCGGGTTTACTTCCCGTCAGGCGTTTGTGCTGATTACCCTTGCCGCAGCACTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAAttt                                                                                                                                                                                                                                               <  1:56253/251‑1 (MQ=255)   aTTTGATCATGCGTGCCGGGTTTACTTCCCGTCAGGCGTTTGTGCTGATTACCCTTGCCGCTGCACTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGTAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTa                                                                                                                                                                                                                                              <  2:20546/251‑1 (MQ=255)       gATCATGCGTGCCGGGTTTACTTCCCGTCAGGCGTTTGTGCTGATTACCCTTGCCGCAGCACTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGCCTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAAcc                                                                                                                                                                                                                                           >  2:203208/1‑250 (MQ=255)             gCGTGTCGGGTTTACTTCCCGTCAGGCGTTTGTTCTGATTACCCTTGCCGCAGCACTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCaaataaa                                                                                                                                                                                                                                    <  1:237041/251‑1 (MQ=255)                    gggTTTACTTCCCGTCAGGCGTTTGTGCTGATTACCCTTGCCGCAGCACTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGAt                                                                                                                                                                                                                             >  2:177336/1‑251 (MQ=255)                       tttACTTCCCGTCAGGCGTTTGTGCTGAGTACCCTTGCCGCAGCACTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGACATGCTGGTGCTCTTTT‑GCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCTTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGAAGTCCCAATCTAACCAAATAAATGAGGAtgtg                                                                                                                                                                                                                          >  2:80089/1‑250 (MQ=255)                                            gtgCTGATTACCCTTGCCGCAGCACTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTgg                                                                                                                                                                                                      <  1:245367/250‑1 (MQ=255)                                                tgattcaccatggcGCAGCACTCCACGCTTCCATTGGCGTGCAGGCAGACTATTTTCATTTTGTACCGGAGTGGGACATGCTCGTGATCTTTTTGATAGCATTCTTCCTCTATGGATATTGAATTAAGCGTGCCTGGAAAGATTCTCGCTTTATTAAGTGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCGCAATTTAACCAAATAAATGAGGATGTGATGAACCAACCAATGCATGGGAAAc                                                                                                                                                                                                 >  2:181102/14‑251 (MQ=255)                                                              gcagcaCTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGaca                                                                                                                                                                                                                    <  2:76833/218‑1 (MQ=255)                                                              gcagcaCTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGaca                                                                                                                                                                                                                    >  1:76833/1‑218 (MQ=255)                                                                agcaCTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGa                                                                                                                                                                                 <  1:219356/251‑1 (MQ=255)                                                                                                  tatTCTAATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTAcc                                                                                                                                               >  1:231454/1‑251 (MQ=255)                                                                                                                        gggTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATgg                                                                                                                          >  2:33269/1‑250 (MQ=255)                                                                                                                        gggTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATgg                                                                                                                          >  2:216839/1‑250 (MQ=255)                                                                                                                          gTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGatt                                                                                                                       >  1:253828/1‑251 (MQ=255)                                                                                                                               gCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTgg                                                                                                                  <  1:106696/251‑1 (MQ=255)                                                                                                                                          ttttGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTg                                                                                                        <  1:177336/250‑1 (MQ=255)                                                                                                                                                taacagtcttcCTCTATGGATATTGCATTACGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAAAGCAGACTGCGTAGACATTGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGATGCTATGGATTATTGGCATGGGGCTGGCGTTTg                                                                                                 >  2:49220/7‑251 (MQ=255)                                                                                                                                                  gCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCg                                                                                               <  2:246405/251‑1 (MQ=255)                                                                                                                                                    aTTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGtt                                                                                             >  2:207171/1‑251 (MQ=255)                                                                                                                                                                       ggCATTAAGCGTGCCTGGAAAGTTGCTCGATTTATTAAGCGCGTAAAACGCAGCCTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATCAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTCCCTTGTGGGCTGGGACGCTATGGATTATTGGCATCGGGCTGTCGTTTGCGTTAATCGCGCTGGCGTATACg                                                                          <  2:16817/250‑1 (MQ=255)                                                                                                                                                                                       ggAAAGTTGCTCGCGTTATGAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTTCCTTGTGGGCTGGGAAGCTATGGATTAGTGGGATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTCa                                                            <  1:36657/249‑1 (MQ=255)                                                                                                                                                                                               gCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTCAGGAGTGGAGc                                                  >  1:77317/1‑251 (MQ=255)                                                                                                                                                                                                           aaGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTCAGGAGTGGAGCTCGACGGCGAtt                                      >  1:154058/1‑251 (MQ=255)                                                                                                                                                                                                               gcgTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTCAGGAGTGGAGCTCGACGGCGATTACCg                                  >  2:221556/1‑251 (MQ=255)                                                                                                                                                                                                                cgTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGACGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGATGGCGTATACGTTTTTTGCTCGTCAGGAGTGGAGCTCGACGGCGATTANCg                                  >  2:252339/1‑250 (MQ=255)                                                                                                                                                                                                                       cGCAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTCAGGAGTGGAGCTCGACGGCGATTACCGATCGTCCa                          <  2:154058/251‑1 (MQ=255)                                                                                                                                                                                                                         cAGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTAAGGAGTGGAGCTCGACGGCGATTACCGATCGTCCAAc                        >  2:170052/1‑251 (MQ=255)                                                                                                                                                                                                                          aGACTGCGTAGAAATCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTCAGGAGTGGAGCTCGACGGCGATTACCGATCGTCCAACg                       >  1:2220/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaTCGTGGTGGCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTCAGGAGTGGAGCTCGACGGCGAGTACCGATCGTCCAACGGTGAATATGCTg           <  1:203208/251‑1 (MQ=255)                                                                                                                                                                                                                                           tggtggCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTCAGGAGTGGAGCTCGACGGCGATTACCGATCGTCCAACGGTGAATATGCTgggggg      >  1:5309/1‑251 (MQ=255)                                                                                                                                                                                                                                               ggCAGTCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTCAGGAGTGGAGCTCGACGGCGATTACCGATCGTCCAACGGTGAATATGCTGGGGGGatat  <  1:166268/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      CCATTTGATCATGCGTGCCGGGTTTACTTCCCGTCAGGCGTTTGTGCTGATTACCCTTGCCGCAGCACTGCTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTCAGGAGTGGAGCTCGACGGCGATTACCGATCGTCCAACGGTGAATATGCTGGGGGGATAT  >  NC_000913/3968758‑3969246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑