Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	556,072	C→T	G161E (GGG→GAG)	ybcI ←	PF04307 family inner membrane protein YbcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	556,072	0	C	T	100.0%	65.0 / NA	23	G161E (GGG→GAG)	ybcI	PF04307 family inner membrane protein YbcI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/13);  total (10/13)

CAGCGAAGTGGCTGAGATTGAAGCGTTAATTCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAAC  >  NC_000913/555840‑556311                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 cAGCGAAGTGGCTGAGATTGAAGCGTTAATTCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAATCACACCcaca                                                                                                                                                                                                                                <  2:172125/251‑1 (MQ=255)          ggCTGAGATTGAAGCGTTAATTCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTc                                                                                                                                                                                                                         >  2:176766/1‑249 (MQ=255)                   tGAAGCGTTAATTCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATc                                                                                                                                                                                                               >  1:202003/1‑250 (MQ=255)                    gAAGCGTTAATTCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCAc                                                                                                                                                                                                             >  1:177166/1‑251 (MQ=255)                              ttCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCg                                                                                                                                                                                                   >  1:76022/1‑251 (MQ=255)                               tCAACAGCGTCTGGATGCCCGTAAAGCGCAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGAAGCATCTCCGGCAACCACACCCACATCACTTCAGAAATAATCACCTGATGCCCGt                                                                                                                                                                                                  >  2:137094/1‑251 (MQ=255)                                           ggATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgtggtg                                                                                                                                                                                       <  1:99098/250‑1 (MQ=255)                                                     aaaGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACa                                                                                                                                                                             <  2:209678/250‑1 (MQ=255)                                                            aaGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGGCAAGGCAAAc                                                                                                                                                                     <  2:177166/251‑1 (MQ=255)                                                                            ggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATc                                                                                                                                                      >  2:152006/1‑250 (MQ=255)                                                                                                                                 cGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACAGTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCGCCTGCCAGGGATCTAAAAAACGTTCAT‑CTGACCACGGTCACAGCCAGCCAACg                                                                                               <  1:17618/251‑1 (MQ=255)                                                                                                                                      gggACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCttt                                                                                          <  1:137094/251‑1 (MQ=255)                                                                                                                                          ccaccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCg                                                                                      <  1:152006/251‑1 (MQ=255)                                                                                                                                            cccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTACCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGcc                                                                                    <  2:85817/250‑1 (MQ=255)                                                                                                                                                   gtcgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGtt                                                                             >  2:49334/1‑251 (MQ=255)                                                                                                                                                                        gCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCCCCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGc                                                        <  1:49334/251‑1 (MQ=255)                                                                                                                                                                            gataggggTTTTCGCATCCGGTTACCGTCTGCGCCACCACAACATTCCATTCTGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGAGGCCCGTACGGCGGGGTTGAACGAGACAATGCAAACGGGGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCTTTCAT‑CTGACCAGGGTCCCAGCCAGCAAACGCTTTTGCCGCCAGTGGTTACCGAATCCATCAAGCTGTGCGATa                                                    <  2:274121/244‑1 (MQ=255)                                                                                                                                                                                      ttCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGAc                                          >  1:73817/1‑251 (MQ=255)                                                                                                                                                                                               ggTTATCGTCTGCGCCACCACACCATGCCCATCAAGAGCATCTCCGGCACCCACCCCCACATCAATTCAGAAAGAATCCCCTGATGCCCTTACGGTGGGGTGTAACGAGACAATTCAAACGGCCCGCCTGTTATCCCCTGCGAGGGAGAGAAAAAGC‑TTTATGCGGCCCACGGTCTCAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTaaaaa                                 <  2:283350/251‑1 (MQ=255)                                                                                                                                                                                                gTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAc                                >  1:198952/1‑251 (MQ=255)                                                                                                                                                                                                          gcgcCACCACAACATCCCCACCGGGCGCATCTCCGGCAACCACTCCCACATCAATTAAGAAATAACCCCCTATTGCCCGGACGGCGTGGTGTAACGAGCAAATGCAAGCGGCGCGACTTTTTTCACCTGCCAGGGAGCGAAAAAGCGTACAT‑CTGACCACGTTCACAGCCAGCCACCGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCagcagc                      <  2:100738/251‑1 (MQ=255)                                                                                                                                                                                                            gcCACCACAACATTCCCATCAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAACTAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCg                     >  1:159701/1‑250 (MQ=255)                                                                                                                                                                                                                               cAGTAGCATCTCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGTCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAAc  <  1:176766/250‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 CAGCGAAGTGGCTGAGATTGAAGCGTTAATTCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT‑CTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAAC  >  NC_000913/555840‑556311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑