Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,589,006	C→T	S471F (TCT→TTT)	acrD →	multidrug efflux pump RND permease AcrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,589,006	0	C	T	100.0%	73.7 / NA	22	S471F (TCT→TTT)	acrD	multidrug efflux pump RND permease AcrD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/10);  total (12/10)

CCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTG  >  NC_000913/2588769‑2589239                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          ccTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTATTACCAttgtt                                                                                                                                                                                                                              <  1:6519/251‑1 (MQ=255)        cATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGa                                                                                                                                                                                                                       <  1:93311/251‑1 (MQ=255)                 gATGGTGCTGGCGATCGGTCTTGTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTTTGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAAGCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCAGCGGTATTTGTTCCAATGGCCTTCTTCGGCGGCTCCACCGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGt                                                                                                                                                                                                              <  2:222820/251‑1 (MQ=255)                                        tggtggATGACGCCATCGTGGTGGTGGAAACCGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTTTTACCATTGTATCGGCGATGGGGCTGTAAGTACTTGTAACGATGATACTc                                                                                                                                                                                       >  2:274945/1‑251 (MQ=255)                                                     cATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATTGCCTTCTTCGGAGGCACCACCGGTGCCATCTATCGCCCGTTCTTTATTACCATTGTTTCGGCGGTGGTGCTCTCCGTACTGGTAGCGATGATCCCCCCTCCCGCTCtgg                                                                                                                                                                          >  2:137472/1‑250 (MQ=255)                                                           ggtggtggAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTTTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCCGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGcc                                                                                                                                                                     >  1:143066/1‑250 (MQ=255)                                                                  gAAAACGGCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACactact                                                                                                                                                             <  2:10239/251‑1 (MQ=255)                                                                   aaaaCGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACtt                                                                                                                                                            <  1:179482/251‑1 (MQ=255)                                                                                     tgagtgagGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGGTGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAggg                                                                                                                                          >  1:53273/1‑250 (MQ=255)                                                                                        gtgagGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAg                                                                                                                                       <  1:20962/251‑1 (MQ=255)                                                                                                   ctcactcCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCCCACTACTTAAGCCACTGAAAAAAGGGGAGCATCATGGGc                                                                                                                             >  1:134864/1‑250 (MQ=255)                                                                                                                                        aGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTTACCAGATGTTTAAc                                                                                       >  1:249875/1‑251 (MQ=255)                                                                                                                                          aTCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCg                                                                                     <  1:155297/251‑1 (MQ=255)                                                                                                                                              ggggggCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAAc                                                                                 <  2:198679/250‑1 (MQ=255)                                                                                                                                                   gCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGa                                                                            <  2:87550/251‑1 (MQ=255)                                                                                                                                                        ggTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGGACCAATGGCCGTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGGTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGGACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCAAACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCCTTTTTTACTGGTTTAACCAGATGTTTAACCGCAAAGCCGAACggc                                                                       >  2:55157/1‑249 (MQ=255)                                                                                                                                                           cGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTTCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTAc                                                                     <  1:227136/250‑1 (MQ=255)                                                                                                                                                                    gATGGTTCTTTCAGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAgggg                                                           >  1:197928/1‑251 (MQ=255)                                                                                                                                                                                                  agCCTTCTTCGGAGGCACCACCGGGGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTTCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAAGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAAAGCCGAACGCTACGAAAAAGAGGTGGCGAAACTTATCCACCGTAGCCTGCGCt                             >  2:10855/2‑251 (MQ=255)                                                                                                                                                                                                          tcGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATtgtg                     >  1:266559/1‑251 (MQ=255)                                                                                                                                                                                                                  accaccGGTGCCATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGCCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGt             >  2:182746/1‑251 (MQ=255)                                                                                                                                                                                                                             cATCTATCGCCAGTTCTTTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTg  >  1:90159/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          CCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTG  >  NC_000913/2588769‑2589239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑