Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,744,751	Δ1 bp	coding (1295/1374 nt)	mdtK →	multidrug efflux pump MdtK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,744,751	0	C	.	100.0%	82.7 / NA	20	coding (1295/1374 nt)	mdtK	multidrug efflux pump MdtK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (9/11);  total (9/11)

CGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGTAACTTCC  >  NC_000913/1744511‑1744988                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              cGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCATATTTCTGGCTCAATCCAGGAGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTGACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCGGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTatg                                                                                                                                                                                                                                     >  2:139106/1‑250 (MQ=255)          cgcATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATAGTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTTAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGc                                                                                                                                                                                                                           <  1:36166/251‑1 (MQ=255)                                       cAGATTTCTGGCTCAATCCAGGTGATTGGTAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTTGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTc                                                                                                                                                                                               <  1:173970/250‑1 (MQ=255)                                             tCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCcat                                                                                                                                                                                         <  1:106521/250‑1 (MQ=255)                                                  cTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATAAGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCAATCATTCt                                                                                                                                                                                   >  1:168521/1‑251 (MQ=255)                                                            gTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGCCCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCACCCATCATTCTGCAACGAGc                                                                                                                                                                          >  1:197547/1‑250 (MQ=255)                                                                          gATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTACGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAg                                                                                                                                                           <  1:135954/251‑1 (MQ=255)                                                                                gCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAgg                                                                                                                                                     <  1:254502/251‑1 (MQ=255)                                                                                                        cATTTTATATATTTCCTTTACGGCTTACTGGGTTCTGGTCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTg                                                                                                                             <  2:150004/251‑1 (MQ=255)                                                                                                         aTTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGt                                                                                                                            >  1:122230/1‑251 (MQ=255)                                                                                                          ttttCTATATTACTTTTACGGCTTACTGGGTGCTGTGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGCGCATCCCGCTAATCAAGACAAGGCGCAACCTTCACGTGTTTCGCCTGt                                                                                                                            >  2:279156/1‑250 (MQ=255)                                                                                                                                                 tGCCAAGCGGTTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGGCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCtt                                                                                     <  1:195500/250‑1 (MQ=255)                                                                                                                                                   ccAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGt                                                                                  <  1:279156/251‑1 (MQ=255)                                                                                                                                                                       aCTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCg                                                              >  2:54327/1‑251 (MQ=255)                                                                                                                                                                                   ggTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGCTAGGCTTTATTATTGGCCTGACGGCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGAGCCCATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACagattt                                                  >  2:145539/1‑248 (MQ=255)                                                                                                                                                                                           aaCCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACt                                          <  1:139106/251‑1 (MQ=255)                                                                                                                                                                                                 tGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGGGCAACCTTCACGGGTTGCGCCTGGATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTCCACAGAGATTGCCGACTGGCATc                                    >  1:130670/1‑251 (MQ=255)                                                                                                                                                                                                          cagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTAtctc                           <  1:254171/251‑1 (MQ=255)                                                                                                                                                                                                                              attagtGGCCTGACGTCGG‑AGCCATTATGATGATTTTGGCTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGGGCCTGTATTTTTACGCAGGCTGGGGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCAGCTGTTATCTCAACGTGTATATCCCCGGTa        <  2:244305/250‑1 (MQ=255)                                                                                                                                                                                                                                   tGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGTAACTTcc  >  2:186798/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              CGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGTAACTTCC  >  NC_000913/1744511‑1744988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑