Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	546,050	C→T	G105D (GGC→GAC)	allD ←	ureidoglycolate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	546,050	0	C	T	100.0%	89.4 / NA	27	G105D (GGC→GAC)	allD	ureidoglycolate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/11);  total (16/11)

TTCCCCATGCCTGTACGGTAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCAC  >  NC_000913/545814‑546296                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       ttCCCCATGCCTGTACGGTAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGc                                                                                                                                                                                                                                           <  1:139436/250‑1 (MQ=255)      cATGCCTGTACGGTAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGtttt                                                                                                                                                                                                                                     >  1:250571/1‑251 (MQ=255)        tGCCTGTACGGTAGCCGCCATATCAAAGGTCAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCTATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTg                                                                                                                                                                                                                                    >  1:99993/1‑250 (MQ=255)                cGGTAGTCGCCATATCAATGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAACTTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCTCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCCTTTTTCTGGGCGGTTTTGATGGCATGt                                                                                                                                                                                                                           >  2:238575/1‑251 (MQ=255)                         ccATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATAccc                                                                                                                                                                                                                  >  2:72562/1‑251 (MQ=255)                           atatCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCAt                                                                                                                                                                                                                >  1:212573/1‑251 (MQ=255)                           atatCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCAt                                                                                                                                                                                                                <  1:37052/251‑1 (MQ=255)                                    gTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTcgccgc                                                                                                                                                                                                       >  1:67660/1‑251 (MQ=255)                                                              cgcgGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCAt                                                                                                                                                                             >  1:198106/1‑251 (MQ=255)                                                                     aaGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAAt                                                                                                                                                                      <  1:208710/251‑1 (MQ=255)                                                                       ggCCAGGGGGTTAGTACCGAAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAACTGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGAACCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTg                                                                                                                                                                    >  2:138451/1‑251 (MQ=255)                                                                                   actcccGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCAGTGTATCGGACGGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTTCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCCGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTCCCACACCCATTTTCGCCGCGACCTGTCCGGCGTCATTGTCGGCGTGTCAAATTGCCGAGCATGGcc                                                                                                                                                        <  2:245474/247‑1 (MQ=255)                                                                                                                                     aCTGGCACATCGAAATGACAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAACACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGa                                                                                                       >  1:73246/1‑250 (MQ=255)                                                                                                                                         gCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGc                                                                                                  <  2:105359/251‑1 (MQ=255)                                                                                                                                             aTCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTc                                                                                              <  2:229846/251‑1 (MQ=255)                                                                                                                                             aTCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTACGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTc                                                                                              >  1:247308/1‑251 (MQ=255)                                                                                                                                               cGAAATGCCAAGGAATCCGTCTCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATACGGCTGATACCGACCACCGCAACGTCATTTTTCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTcc                                                                                             <  2:11969/250‑1 (MQ=255)                                                                                                                                                   aTGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCgta                                                                                        <  1:138451/251‑1 (MQ=255)                                                                                                                                                         ataaaTCCGGAGCGGGCTGCCTGCTGCACAAAATAAGAGCTTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCACCGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCCTTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGATTCGTCAAGACGAAACTCCGGTTCCCGGTTGGTGCAGCCTTTTACAATGCGTTCCGCGTAGTATTc                                                                                  >  2:271548/4‑251 (MQ=255)                                                                                                                                                                   cgcgGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCAcc                                                                        <  2:207838/251‑1 (MQ=255)                                                                                                                                                                               gctgcACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACAGCAACGTCATTCTGCTGGGCGGTTTTGATGGCATGTTCCATACCCCTTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCACCTTTTGAAATGCGTTCCGCGTAGTATTCAACGCGCACCGCGCCATgag                                                              >  1:111369/1‑249 (MQ=255)                                                                                                                                                                                  gcACAAAATAAGAGATTGCGCCGCTGTGAACCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGAGTAGTATTCCACGCGTACAGGGCCATGAGAGTgg                                                         >  1:136166/1‑251 (MQ=255)                                                                                                                                                                                         aTAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATCCCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCAGCCTTTTGAAATGCGTTCCGCGTCGTATTACACGCGCACCGCGCCATGAGAGTGGATCCctc                                                  >  2:198521/1‑251 (MQ=255)                                                                                                                                                                                           aaGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCctct                                                 <  2:198106/250‑1 (MQ=255)                                                                                                                                                                                                             gACCCATCCGGCGGATACCGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGGCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGGACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGATATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAAt                              <  2:221223/251‑1 (MQ=255)                                                                                                                                                                                                                              ccGACCACCGCAACGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGc             >  2:159744/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaCGTCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCAc  >  2:245924/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       TTCCCCATGCCTGTACGGTAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCAC  >  NC_000913/545814‑546296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑