Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,368,016	C→T	P316S (CCA→TCA)	arnC →	undecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,368,016	0	C	T	100.0%	31.7 / NA	12	P316S (CCA→TCA)	arnC	undecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/3);  total (9/3)

TTAGCATTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTGTGGGTGGAACGCATTGCCCAACT  >  NC_000913/2367813‑2368259                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    ttAGCATTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTTCGCGCCCGCCCCCGCTATTTTTTTCAGCAAGTTATCCGGTCATCCAGCAAGGAAAATGAATAATGAAAAACGTCGTTTTTGCCTAcc                                                                                                                                                                                                      >  2:65692/1‑251 (MQ=255)        tGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTTCATCCAGCAAGGAAAATGAATAATGAAAACAGTCGTTTTTGCCTACCACGatat                                                                                                                                                                                               >  2:207409/1‑251 (MQ=255)                                                             aaGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGGCCCCGCTATTTTGTTCAGCAAGTTATCCGTTCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATAAGGGATGCCTCGGGATTGAAGCACTGCTGGCTGCCGGCTAAGAAATTAGCGcc                                                                                                                                           >  2:121980/1‑250 (MQ=255)                                                             aaGGCGTCTTTATGATATTTGCCGAGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTTCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGGCCTGCTGGCTGCCGGTTACGAAATTAGCGccc                                                                                                                                          >  2:188582/1‑250 (MQ=255)                                                                                 gCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTTCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAAt                                                                                                                      <  2:104475/251‑1 (MQ=255)                                                                                      gCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTTCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCgg                                                                                                                 <  1:269656/251‑1 (MQ=255)                                                                                       cTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTTCATCCAGCAAGGAAAATGAATAATGAAAACCGGCGTTTTTGCCTACCACGATATGGGATGCCTCGGAATTGAATCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCggg                                                                                                                >  2:111730/1‑250 (MQ=255)                                                                                                tttATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCGGCACCCGCACCCGCTATTTTGTTCAGCAAGTTACCCGTTCATCCAGCAAGGAAAAGGAATAATGAAAACAGTAGGTTTTGCCTACCACGATCTGGGGTGCCCCGGTATTAAAGCCCTGCTTGCTGCCGGGTAAGCACTTAGCGCCATTTTTACCCAGACTGATAAGCCCGGTGAGAAAGcc                                                                                                       >  1:252982/1‑251 (MQ=255)                                                                                                                         tGGGATTACTAGGTGAATATATCGGCAGGATATACACCGATGTCAGCGCCCGCACCCGCTAGTTTGTTCAGCAAGTTATCCGTTAATCCAGCAAGGAAAATGAATAATGAAAACAGTCGTTTTTGCCTATCACGATATAGGATGCCTCGGTAGTGAAGCCGTTCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTAACCATACTGATAATCCAGGTGGAAAAGCATTTTATGGTTAGGGGGCTCGTCTgg                                                                              >  1:89184/1‑251 (MQ=255)                                                                                                                                                ggCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTTCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGtt                                                       >  1:18252/1‑251 (MQ=255)                                                                                                                                                              cGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTTCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATa                                          >  1:268902/1‑250 (MQ=255)                                                                                                                                                                                                      aTCCGTTCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTGTGGGTGGAACGCATTGCCCAACt  <  1:79018/250‑1 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    TTAGCATTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTGTGGGTGGAACGCATTGCCCAACT  >  NC_000913/2367813‑2368259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑