Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,287,391	G→A	intergenic (‑20/‑220)	acs ← / → ytcB	acetyl‑CoA synthetase (AMP‑forming)/protein YtcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,287,391	0	G	A	100.0%	34.0 / NA	11	intergenic (‑20/‑220)	acs/ytcB	acetyl‑CoA synthetase (AMP‑forming)/protein YtcB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/8);  total (3/8)

CGTCCTCGTACCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCAC  >  NC_000913/4287155‑4287616                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                  cGTCCTCGTACCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGAATGTTAATAATAtg                                                                                                                                                                                                                     >  1:283312/1‑251 (MQ=255)                                            ggTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGAATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAg                                                                                                                                                                          <  1:226041/251‑1 (MQ=255)                                                             ggTAAGGTTTAATCCCGTCACGGATTTTTCCCTGTTCGCACCAGAAGGTATCAGGCCCGTTAATAGATTGTTGATACATCGCCCCGTACTGCTGAAGGGTTATCCGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCACGCTTTTGTTCTCCTTGTAGAATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGAc                                                                                                                                                              >  2:24435/1‑246 (MQ=255)                                                                  ggTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGAATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCa                                                                                                                                                    <  2:190853/251‑1 (MQ=255)                                                                                        ttCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGAATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATaa                                                                                                                               <  1:168767/250‑1 (MQ=255)                                                                                                    cccAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGAATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAt                                                                                                                  >  1:96505/1‑251 (MQ=255)                                                                                                                                                                ttATCAGGCAACGGTCGGCGATGATGGCAGGAATGGTGTTTTTGTGAAGTTGGCTCATGCTTTTGTTCTCCTTGTAGAATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGGTTTGTTTAGTATTTGGGCGACAGATCTCGTAAAAGTAGAATTGTGATGATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCGTGGAGGAACATGCAGTTatgcatgc                                                       <  2:48844/250‑1 (MQ=255)                                                                                                                                                                   acAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGGTTGTGAATTTGGCTCATGCTGTTGTTCTCCTTGTAGAATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCATCAACAAAAGTTGATTAAGTCTTTGAGGAACATACAGTTATGCATGCtttt                                                   <  2:126147/250‑4 (MQ=255)                                                                                                                                                                          aCGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGAATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGGTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAag                                            <  2:283312/251‑1 (MQ=255)                                                                                                                                                                                                               aTTTGGCTCATGCTTTTGTTCTCCTTGTAGAATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGGTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACa       <  1:271098/251‑1 (MQ=255)                                                                                                                                                                                                                    gCTCATGCTTTTGTTCTCCTTTTAGAATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTTGGCGTCAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAATGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCAc  <  1:155500/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                  CGTCCTCGTACCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCAC  >  NC_000913/4287155‑4287616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑