Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	275,932	G→A	G203D (GGC→GAC)	mmuP →	S‑methyl‑L‑methionine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	275,932	0	G	A	100.0%	88.4 / NA	28	G203D (GGC→GAC)	mmuP	S‑methyl‑L‑methionine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/13);  total (15/13)

ACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGG  >  NC_000913/275693‑276173                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  acaGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGAttt                                                                                                                                                                                                                                        >  2:137326/1‑251 (MQ=255)                 gtcgggTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCGCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTAtggtggt                                                                                                                                                                                                                        <  2:90927/247‑2 (MQ=255)                         cGTNGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACt                                                                                                                                                                                                                <  2:252848/251‑1 (MQ=255)                                       cgATTATTTTTGGGCTGAATGTTATCACCACTCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGt                                                                                                                                                                                                  <  2:142708/251‑1 (MQ=255)                                         attattTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGCCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGCTGACTATTGTTGCAGTGACCTTTGCTTTTTCGGTTAc                                                                                                                                                                                                >  1:239959/1‑251 (MQ=255)                                                       gAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGc                                                                                                                                                                                   >  1:75078/1‑250 (MQ=255)                                                                  ccACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCggg                                                                                                                                                                       >  2:102106/1‑250 (MQ=255)                                                                     cgcgCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTa                                                                                                                                                                                                 >  1:152133/1‑222 (MQ=255)                                                                     cgcgCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTa                                                                                                                                                                                                 <  2:152133/222‑1 (MQ=255)                                                                             tttGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGaaa                                                                                                                                                             >  2:52879/1‑250 (MQ=255)                                                                                gCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAAccc                                                                                                                                                         <  2:194691/251‑1 (MQ=255)                                                                                                ggTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATcc                                                                                                                                           <  1:65823/249‑1 (MQ=255)                                                                                                   tctcGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGt                                                                                                                                       >  1:64969/1‑250 (MQ=255)                                                                                                                         tATCATCGCCTTTATCATACTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAACGTTATCCCGGTAGCGATTCGTACTACCATcgcgc                                                                                                                >  2:272620/1‑251 (MQ=255)                                                                                                                                 ccTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGatt                                                                                                        >  1:234886/1‑251 (MQ=255)                                                                                                                                                       ggcgATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCgtgt                                                                                  <  1:67615/251‑1 (MQ=255)                                                                                                                                                           aTTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGCAACGGCAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTtgg                                                                              >  1:39225/1‑250 (MQ=255)                                                                                                                                                                     ttATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAgc                                                                     <  1:102106/250‑1 (MQ=255)                                                                                                                                                                                          cGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCCCGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCGGGCAGCGCTGATCCCGATGCAGCAGGt                                                <  1:222049/250‑1 (MQ=255)                                                                                                                                                                                           gCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTgg                                              >  2:18198/1‑251 (MQ=255)                                                                                                                                                                                              cgcgCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCg                                           <  1:137326/251‑1 (MQ=255)                                                                                                                                                                                                 gcCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTgg                                                                         >  2:146300/1‑218 (MQ=255)                                                                                                                                                                                                 gcCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTgg                                                                         <  1:146300/218‑1 (MQ=255)                                                                                                                                                                                                           aGTAATATCACGGCAGAATGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGccc                              <  1:124557/251‑1 (MQ=255)                                                                                                                                                                                                                      ggCAGAAGGCTGGTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCCAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTgg                   >  2:245797/1‑251 (MQ=255)                                                                                                                                                                                                                       gCAGAAGGCTGGTTCCCGCCCGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGATCTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTGATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTTGGCGTGGAGAAAAGCCCGTTTGTGCTGGt                  <  1:90397/251‑1 (MQ=255)                                                                                                                                                                                                                                 ggTTCCCGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTgagga         >  2:58448/1‑248 (MQ=255)                                                                                                                                                                                                                                       cGCACGGTGACTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAggg  >  2:220793/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  ACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGG  >  NC_000913/275693‑276173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑