Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,701,959	C→T	G171R (GGG→AGG)	recO ←	recombination mediator protein RecO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,701,959	0	C	T	100.0%	115.1 / NA	34	G171R (GGG→AGG)	recO	recombination mediator protein RecO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/15);  total (19/15)

ACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCCTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTC  >  NC_000913/2701714‑2702190                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        aCAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGATTGCGATAAACCtttttt                                                                                                                                                                                                                                    >  2:119320/1‑251 (MQ=255)    gTAATTCAGCCATGACAATCCTCAGCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTttctt                                                                                                                                                                                                                                 <  1:164731/251‑1 (MQ=255)     tAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTttcttc                                                                                                                                                                                                                                >  1:161830/1‑251 (MQ=255)             ccATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAAc                                                                                                                                                                                                                        <  1:213764/251‑1 (MQ=255)               aTGACAATCCTCATCCTTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTTAACGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAACGTTTCGCGGCACGCAGTGTGTCTGCGTCGGGAAATTCCCGTGCGCTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTAGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACg                                                                                                                                                                                                                       >  1:60070/1‑250 (MQ=255)                       ccTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCa                                                                                                                                                                                                               <  2:172275/250‑1 (MQ=255)                          catcagTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAAAGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGgt                                                                                                                                                                                                           >  2:57363/1‑251 (MQ=255)                                 catAAGGTGTTTTTACCGCGCGCTTAGGCATAAAATACCGGAACAGTTCCCTGCTCTTTAAATGTTTACCGCCAAGATACGGCTTAAGCGACATGCGGGTAAAGCGTTTCTCTGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTGCTTCGCGAGAACGATACGTCATTGTGTCATc                                                                                                                                                                                                     <  2:60070/250‑1 (MQ=255)                                  atAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTa                                                                                                                                                                                                   <  1:197253/251‑1 (MQ=255)                                            ttCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGACCGTTTTATTGCCATTAACGCCGCTTCGGATAAACCTTTTTTCTTCGCGTTAACAAAACGTAATGGTGTCTTCTACCgccccgcc                                                                                                                                                                                         >  2:223601/1‑244 (MQ=255)                                                 cGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTAc                                                                                                                                                                                    <  1:119320/251‑1 (MQ=255)                                                                  aCTGCCGGAACAGATCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGGTACCCGCAAAATGGGGaaa                                                                                                                                                                    >  2:243073/1‑250 (MQ=255)                                                                                 cccTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAAcc                                                                                                                                                     <  1:86859/250‑1 (MQ=255)                                                                                            aaaGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGGAAAATTGACGCCATAACCCAGATGCCCg                                                                                                                                           >  1:33405/1‑249 (MQ=255)                                                                                             aaGGTTTACCGCCAAGAGACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCTCTCAGTGTGTCTGCGGCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACTATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGc                                                                                                                                        <  2:277864/251‑1 (MQ=255)                                                                                                                cGGCTGAAGCGCCATGCGGGGAGAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACTCTTTTAACTGCCTTTCGGTGACCGTTTTATTGTCGATAACGACGCTTGCGATAAACCTGGTTGCTTCGCGAGAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGTACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAgcggc                                                                                                                     <  1:25639/251‑1 (MQ=255)                                                                                                                  gCTTAAGCGACAAGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGATTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCAGCTATCCGCTACCCCAACAATGGGGAAAATTTACGCCATAACCCCGATGCCCGAGCAGTGCCAGttaacaccggcgc                                                                                                                   >  2:274780/1‑240 (MQ=255)                                                                                                                    ttAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTATCTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACACTGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGgcgc                                                                                                                   >  2:134367/1‑249 (MQ=255)                                                                                                                                    tAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAACTTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACATTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTcgcgc                                                                                                  >  1:256289/1‑249 (MQ=255)                                                                                                                                     aaaGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTAc                                                                                                >  2:108887/1‑251 (MQ=255)                                                                                                                                                  gcgcgcAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGc                                                                                   >  1:38680/1‑251 (MQ=255)                                                                                                                                                      gcAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCATTTCAAAGCGGCGCCGCCCGGGTTCTGGCGTACCCGTGACCCCTGCaaa                                                                                >  2:176953/1‑249 (MQ=255)                                                                                                                                                             gtCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAAc                                                                                                                                                      <  1:221315/173‑1 (MQ=255)                                                                                                                                                             gtCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGAAGCCATAAc                                                                                                                                                      >  2:221315/1‑173 (MQ=255)                                                                                                                                                                              cccGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATc                                                        <  1:57363/250‑1 (MQ=255)                                                                                                                                                                                           cGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTc                                            >  2:31022/1‑249 (MQ=255)                                                                                                                                                                                                   aCTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAaga                                                                               >  2:209448/1‑206 (MQ=255)                                                                                                                                                                                                   aCTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAaga                                                                               <  1:209448/206‑1 (MQ=255)                                                                                                                                                                                                            ccgttacccgtttATTGGCGAGAACGACGCTTGCTATAAACCTTGTTTGATCCGGAGTACGATACGTCATAGTGTCATCTACCGGCTCGCCGCTAACCGCACAATGGGGAAAAGGCGCGCCATAACCTAGATGCCCGAGCAGGGCCAGTGCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTTACCCCTGCAAGAGACTTAATGCAGTTCAATTAATCGAAAAAAAGTTCTGAGAAgcgcatcgcttat                         <  2:45235/241‑10 (MQ=255)                                                                                                                                                                                                                   cGTTTTATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGAAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCCGAGAAGCGCGTCTCGTATTCCAGt                   >  2:241360/1‑250 (MQ=255)                                                                                                                                                                                                                      tttATTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGAGACAGTTCAAAGCGGCGCAGAGCGGGTTCTGGCGTACCAGGGACCCCTGCAAGAGACTGAATGCAGTGCAAGAAATCGAACAAAAGTTCAGAGAAGCGCGTCCCGAATTCCAGTAcgc               >  2:224050/1‑251 (MQ=255)                                                                                                                                                                                                                         aTTGTCGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCggg            <  1:37340/251‑1 (MQ=255)                                                                                                                                                                                                                              cGATAACGACGCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGgag          >  1:139049/1‑248 (MQ=255)                                                                                                                                                                                                                                   acgacgCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCTCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCCGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCCGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGACGTTc  >  2:83850/1‑251 (MQ=255)                                                                                                                                                                                                                                   acgacgCTTGCGATAAACCTTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTc  <  1:179979/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        ACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTGCGATAAACCCTTTTTCTTCGCGATAACGATACGTCATGGTGTCATCTACCGGCTCGCCGCTACCCGCACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTC  >  NC_000913/2701714‑2702190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑