Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,981,902	C→T	S339F (TCT→TTT)	yifK →	putative transporter YifK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,981,902	0	C	T	100.0%	96.8 / NA	28	S339F (TCT→TTT)	yifK	putative transporter YifK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/20);  total (8/20)

ACCATCTTCCCGTGGAATGAAATAGGCAGCAACGGCAGCCCGTTCGTACTGACTTTTGCCAAAATCGGTATTACCGCAGCGGCGGGCATTATCAACTTTGTGGTGCTGACGGCTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATAAGCCAGCTGCGTTTTCGGCGTGCACATAAAGCGGCGATTGCCAGCCATCCGTTCCGCTCAATCCTGTTCCCGTGGGCCAATTACGTAACAATGGCATTCCTGATTTGCGTTTTGATCG  >  NC_000913/3981655‑3982144                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    cccATCTTCCCGTGGAATGAAATAGGCAGCAACGGCAGCCCGTTCGTACTGACTTTTGCCAAAATCGGTATTACCGCAGCGGCGGGCATTATCAACTTTGTGGTGCTGACGGCTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTAt                                                                                                                                                                                                                                                  <  2:19865/250‑1 (MQ=255)                        aGGCAGCAACGGCAGCCCGTTCGTACTGACTTTTGCCAAAATCGGTATTACCGCAGCGGCGGGCATTATCAACTTTGTGGTGCTGACGGCTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑At                                                                                                                                                                                                                          <  1:110779/251‑1 (MQ=255)                                      gCCCATTCGTACTGACTTTTGTTAAAATCGGTATTACCGCAGCGGAGGGCATTATCAACTTTGTGGTGCTGACGGCTTAGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCAGTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCGGAACTAcatc                                                                                                                                                                                                            <  1:147363/251‑1 (MQ=255)                                       cccGTTCGTACTGACTTTTGCCAAAATCGGTATTACCGCAGCGGCGGGCATTATCAACTTTGTGGTGCTGACGGCTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTAcatca                                                                                                                                                                                                           <  1:85662/251‑1 (MQ=255)                                                aCTGACTTTTGCCAAAATCGGTATTACCGCAGCGGCGGGCATTATCAACTTTGTGGTGCTGACGGCTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAAt                                                                                                                                                                                                   <  1:257333/250‑1 (MQ=255)                                                   gACTTTTGCCAAAATCGGTATTACCGCAGCGGCGGGCATTAGCAACTGTGTGGTGCTGACGGCTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATcc                                                                                                                                                                                                 <  1:257077/249‑1 (MQ=255)                                                    aCTTTTGCCAAAATCGGTATTACCGCAGCGGCGGTCATTATCAACTTTGTGGTGCTGACGGCTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCa                                                                                                                                                                                              <  1:278486/251‑1 (MQ=255)                                                                   ggTATTACCGCAGCGGCGGGCATTATCAACTTTGTGGTGCTGACGGCTGCGCTCTCTGGCTGTAACAGCGGCATGGACAGTTGCGGGCGGGTGGTCTACGCACTGGCGAAAAACCGGCCGGTAACGGGGGCAATGGGGGAAGGTTCCCGGCACGGCGGACCGGGTGCGGGGGTGGGAGGGTTTTTTGCTATTCTGGTAAATTGCTC‑ATGCCTGGACTTCATCATTCCCCAACCCCAGCGTGTGGTTgtctt                                                                                                                                                                               >  1:140999/1‑250 (MQ=255)                                                                                  gcggGCATTATCAACTTTGTGGTGCTGACGGCTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAAGGGCGAAAGATTCCCGTCACGGCCTACCGGTTGCGGGGGTGGCCGTATTTATTGGTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATACCCAGCGTGGGTTTGTTTACGGCTACAGTGcc                                                                                                                                                                  >  1:284708/1‑249 (MQ=255)                                                                                   cggGCATTATCAACTTTGTGGTGCTGACGGCTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGc                                                                                                                                                               <  2:22980/251‑1 (MQ=255)                                                                                        aTTATCAACTTTGTTGTGCTGACGGCTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTTGCGAAAAACCGTCAGTTACCGGGGGCCATTGCGAAAGTTTCCCGTCCCCGCATACCGGTTGCGGGTGTGGCAGTATTTATTTCTCTTCTGCTTATTGGCTCCATGCTTGAA‑TAAATCATTCCCCATCCGCCGCGTGTTTTTTTCTTCGTCTCCATTTCCAGCGTGCt                                                                                                                                                          >  2:170470/1‑251 (MQ=255)                                                                                                      ggtgCTGACGGCTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGc                                                                                                                                            <  1:135892/251‑1 (MQ=255)                                                                                                                   gctctctttGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCTTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGa                                                                                                                                <  1:172162/247‑1 (MQ=255)                                                                                                                      ctctctGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTccc                                                                                                                                                                                                      <  1:204623/177‑1 (MQ=255)                                                                                                                                gTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAACACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCCTTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGTTAAGcc                                                                                                                    >  1:40116/1‑249 (MQ=255)                                                                                                                                gTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATAAGcc                                                                                                                    <  2:40116/249‑1 (MQ=255)                                                                                                                                           aTGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATAAGCCAGCTGCGTTTTCg                                                                                                       <  1:144958/251‑1 (MQ=255)                                                                                                                                                cAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATAAGCCAGCTGCGTTTTCGGCGTg                                                                                                  <  1:79152/251‑1 (MQ=255)                                                                                                                                                            tatactctACGCACTGGCGAAAAACCGTAAGTTACGGGCGGCAATGGCGAAAGTTTCCAGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTCTTCTGCTAATTGGCTA‑ATGCCTGTACTACATCATTACAAATCCACAGCGTGGGTTTGTCTACGTCTACAGTGCCAGCGAGCTTGCGGGGATGGGGCCATGGTTTGTGATATTGGTAAGAAAGCTGGGTTTTGGGCGGGCACATTAAgcgg                                                                                      >  2:128994/5‑251 (MQ=255)                                                                                                                                                                            gCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATAAGCCAGCTGCGTTTTCGGCGTGCACATAAAGCGGCGATTGCCAGCCATcc                                                                      <  2:183012/251‑1 (MQ=255)                                                                                                                                                                                 aaaCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATAAGCCAGCTGCGTTTTCGGCGTGCACATAAAGCGGCGATTGCCAGCCATCCGTTcc                                                                 <  2:136511/251‑1 (MQ=255)                                                                                                                                                                                               cggcggCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATAAGCCAGCTGCGTTTTCGGCGTGCACATAAAGCGGCGATTGCCAGCCATCCGTTCCGCTCAATCCTGTTc                                                   >  2:104323/1‑251 (MQ=255)                                                                                                                                                                                                   ggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATTGTTTGTGATATTGATAAGCCAGCTGCGTTTTCGGCGTGCGCATAAAGCGGCTATGGCCAGCCATCCGTTCCGCTCGATCCGGTTCCCGt                                               <  2:238159/251‑1 (MQ=255)                                                                                                                                                                                                             aaaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑CTGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATAAGCCAGCTGCGTTTTCGGCGTGCACATAAAGCGGCGATTTCCAGCCATCCGTTCCGATCAATCCTGTTCCCGTGGGCCAAtt                                      >  2:23095/1‑250 (MQ=255)                                                                                                                                                                                                                               gTACCGGTTGCGGGTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATAAGCCAGCTGCGTTTTCGGCGTGCACATAAAGCGGCGATTGCCAGCCATCCGTTCCGCTCAATCCTGTTCCCGTGGGCCAATTACGTAACAATGGCATTCCt                   <  1:31537/251‑1 (MQ=255)                                                                                                                                                                                                                                          gggTGTGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTAAATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATAAGCAAGCTGCGTTTTCGGCGTGCACATAAAGCGGCGATTGCCAGCCATCCGTTCCGCTCAATCCTGTTCCCGTGGGCCAATTACGTAACAATGGCATTCCTGATTTGCGttt        >  1:111522/1‑251 (MQ=255)                                                                                                                                                                                                                                            gtgtGGCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATAAGCCAGCTGCGTTTTCGGCGTGCACATAAAGCGGCGATTGCCAGCCATCCGTTCCGCTCAATCCTGTTCCCGTGGGCCAATTACGTAACAATGGCATTCCTGATTTGCGTTTTg      <  1:122470/251‑1 (MQ=255)                                                                                                                                                                                                                                                ggCAGTATTTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATAAGCCAGCTGCGTTTTCGGCGTGCACAGAAAGCGGCGATTGCCAGCCATCCGTTCCGCTCAATCCTGTTCCCGTGGGCCAATTACGTAACAATGGCATTCCTGATTTGCGTTTTGATCg  <  1:170470/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    ACCATCTTCCCGTGGAATGAAATAGGCAGCAACGGCAGCCCGTTCGTACTGACTTTTGCCAAAATCGGTATTACCGCAGCGGCGGGCATTATCAACTTTGTGGTGCTGACGGCTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTC‑ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATAAGCCAGCTGCGTTTTCGGCGTGCACATAAAGCGGCGATTGCCAGCCATCCGTTCCGCTCAATCCTGTTCCCGTGGGCCAATTACGTAACAATGGCATTCCTGATTTGCGTTTTGATCG  >  NC_000913/3981655‑3982144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑