Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,992,961	C→T	N603N (AAC→AAT)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,992,961	0	C	T	100.0%	101.1 / NA	30	N603N (AAC→AAT)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/18);  total (12/18)

GTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCG  >  NC_000913/3992722‑3993193                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          gTCGCCGACGTGTCGCACCAATTACCTCTGCGCTTTCCTGCACCAGCACCGAAGGCGCTCTACTGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACGCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGCATGAAGTGCGTAc                                                                                                                                                                                                                                <  2:146863/251‑1 (MQ=255)   cGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACg                                                                                                                                                                                                                               <  1:250524/250‑1 (MQ=255)   cGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGc                                                                                                                                                                                                                              <  1:67394/251‑1 (MQ=255)     ccGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTg                                                                                                                                                                                                                            <  2:38920/251‑1 (MQ=255)                   cgtntCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGGGAGATCCGCCATCTGGCTATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCGTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTTCCGCAGCTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGa                                                                                                                                                                                                              <  2:256374/249‑1 (MQ=255)                       tCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGATGCACTTCAACGGCGAGcc                                                                                                                                                                                                           >  2:195476/1‑249 (MQ=255)                              gcgcTTACCAGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCGGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAGCTCGTGGAATGAAGTGCGTACGCTGCTCTTCAACGGCGAGCAATCgat                                                                                                                                                                                                     <  2:98091/249‑1 (MQ=255)                                    aCCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGaa                                                                                                                                                                                              <  1:46337/250‑1 (MQ=255)                                                                          agaTCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCTCTTCAACGGCGAGCAAGCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGAcgcc                                                                                                                                                       <  1:207343/251‑1 (MQ=255)                                                                                       ggCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGAt                                                                                                                                           <  1:54935/250‑1 (MQ=255)                                                                                                            atatGACCCGTCAGCTGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTtata                                                                                                                    <  2:238875/251‑1 (MQ=255)                                                                                                                     gACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGc                                                                                                                                                                         <  1:82996/190‑1 (MQ=255)                                                                                                                     gACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGAAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGc                                                                                                                                                                         >  2:82996/1‑190 (MQ=255)                                                                                                                          cggcgTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTgcgc                                                                                                      >  2:126175/1‑251 (MQ=255)                                                                                                                          cggcgTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTgcgc                                                                                                      <  2:34563/251‑1 (MQ=255)                                                                                                                              gTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTGCGCGGCt                                                                                                  >  1:149076/1‑251 (MQ=255)                                                                                                                                                  aTTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGa                                                                                                                                   >  1:226984/1‑199 (MQ=255)                                                                                                                                                  aTTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGa                                                                                                                                   <  2:226984/199‑1 (MQ=255)                                                                                                                                                                            ttCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAAtt                                                    >  1:191703/1‑251 (MQ=255)                                                                                                                                                                             tCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTGCCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCAGCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAAt                                                     <  1:183654/249‑1 (MQ=255)                                                                                                                                                                             tCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAAt                                                     <  1:9077/249‑1 (MQ=255)                                                                                                                                                                              cAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTg                                                   <  1:105943/250‑1 (MQ=255)                                                                                                                                                                                                   ttGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGCATTGAATGGCGTCTTCCCAGCACCCGCCAgg                             >  2:223253/1‑251 (MQ=255)                                                                                                                                                                                                            aggtagCGTTGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGc                    >  2:174198/1‑251 (MQ=255)                                                                                                                                                                                                              gtagCGTTGACCTGCTGTCCCGCAACTCGTGGAATGAAGTGCGGACGCTGCACTTCAACCGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGCTAGCGCGGAAGTC‑TTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTATCTGAGTGTCTTGAATTGCGTCTTTCCAGCACCCTCCATGAACCCGGGCGt                  >  2:4412/1‑251 (MQ=255)                                                                                                                                                                                                              gtagCGTTGACCTGCTGTACCGCAGCTCGTGGAATGAAGTGCGTACGCTGCACTTCAAAGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCCGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGCATTGCGTCTTTCCAGCATCCCCCAGTAATCCGGGCgc                  >  2:265917/1‑250 (MQ=255)                                                                                                                                                                                                                    ttGACCTGCTGTACCGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCa              >  1:68530/1‑249 (MQ=255)                                                                                                                                                                                                                              gtacggcagtccGTGGAATGAAGTTCGAACTCGGCATTTCAACGGAGAACAATGGATGTTCAAAGCCCTGAAAACTATTCTCGGCAAAAGGCATCAGGAGGCCGCCCCGCCAGATAGCGGGGAA‑TCGTTCGTTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGTTTTCTGAGTATTTTGAATTGCGTCTTTCAAGCTCCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGcgcg  <  2:8298/240‑1 (MQ=255)                                                                                                                                                                                                                                  cGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTGCGCGGCTTAAtt                                                                                             >  2:37518/1‑156 (MQ=255)                                                                                                                                                                                                                                  cGCAACTCGTGGAATGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTGCGCGGCTTAAtt                                                                                             <  1:37518/156‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          GTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTC‑TTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCG  >  NC_000913/3992722‑3993193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑