Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,952,440	C→T	T80T (ACC→ACT)	ilvM →	acetolactate synthase II subunit IlvM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,952,440	0	C	T	100.0%	145.9 / NA	44	T80T (ACC→ACT)	ilvM	acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/21);  total (23/21)

AATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCC  >  NC_000913/3952194‑3952675                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            aaTTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACAt                                                                                                                                                                                                                                         <  1:79459/251‑1 (MQ=255)  aTTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATc                                                                                                                                                                                                                                        <  1:230272/251‑1 (MQ=255)         tgatgCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCACCGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATATGCCAGAGCACAACTAAATCacaaca                                                                                                                                                                                                                                  >  1:129397/1‑250 (MQ=255)          gatgCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGGGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCAccacaa                                                                                                                                                                                                                                 >  2:34899/1‑250 (MQ=255)               aaCATCAGGTCAATGTATCGGCTCGCTTCAATCAAGAAACCTTAGAACGTGTTTTACGCGTGGGGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGGCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATATGCCAGAGAAAAACTACATAACAACAAATCcgc                                                                                                                                                                                                                           >  1:121941/1‑251 (MQ=255)                aCATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATCTGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACCACTACATCACAACAAAACcgcg                                                                                                                                                                                                                          >  2:156061/1‑251 (MQ=255)                    cAGGTCAATGTATCGGCTCGCTTCAAGCAAGAAACCTTAGAAAGTGTTTTACGCGGGGTGCGTCATCGGGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGAGATGCACAAAATATAAAAATCCAATTGACCGTTGCCAGCCCACGGTCGGTCGACTGACTGTTTAGTCAGTTAAATAAAATGGTGGACGACGCACACGATGCCATCTGCCAGAGCACAACTACATCACAACAAATcagccactg                                                                                                                                                                                                                      >  1:203700/1‑243 (MQ=255)                       gTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAgc                                                                                                                                                                                                                   <  2:181597/251‑1 (MQ=255)                          aaTGTATCTGCTCGCTTCAATCCAGAATCCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCa                                                                                                                                                                                                                <  1:141047/251‑1 (MQ=255)                           aTGTATCGGCTCGCTTCCATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACCAAAAATCCGCGCCTGAGCGCa                                                                                                                                                                                                                >  2:189354/1‑250 (MQ=255)                            tGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGGGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCaa                                                                                                                                                                                                               >  2:93707/1‑250 (MQ=255)                               aTCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATTAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGACAGCCCAAGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAgg                                                                                                                                                                                                           >  1:34107/1‑251 (MQ=255)                                                  gAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACg                                                                                                                                                                                         >  2:85773/1‑250 (MQ=255)                                                         tAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTGTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGaaa                                                                                                                                                                                   <  1:65451/249‑1 (MQ=255)                                                            aaCGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGa                                                                                                                                                                              <  1:156061/251‑1 (MQ=255)                                                               gtgtTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGAtt                                                                                                                                                                            >  1:122827/1‑250 (MQ=255)                                                                  ttttACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCAACTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACa                                                                                                                                                                         <  2:222856/250‑1 (MQ=255)                                                                   tttACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACAt                                                                                                                                                                        <  1:142747/250‑1 (MQ=255)                                                                                               cACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGAACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACCACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTggg                                                                                                                                           <  2:204958/251‑1 (MQ=255)                                                                                                aCGTCTGCTCAATGAATATGGCCGCCTCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGTCCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGa                                                                                                                                          <  1:273270/251‑1 (MQ=255)                                                                                                  gTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAg                                                                                                                                        <  1:189354/251‑1 (MQ=255)                                                                                                    cTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGAc                                                                                                                                      >  1:92332/1‑251 (MQ=255)                                                                                                               atatGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCa                                                                                                                           <  1:48642/251‑1 (MQ=255)                                                                                                                                       aaaaTATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGaa                                                                                                                                         >  1:262435/1‑213 (MQ=255)                                                                                                                                       aaaaTATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGaa                                                                                                                                         <  2:262435/213‑1 (MQ=255)                                                                                                                                       aaaaTATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACt                                                                                                    >  1:166153/1‑250 (MQ=255)                                                                                                                                                      aaTTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGAc                                                                                                                                                                                             >  1:41232/1‑146 (MQ=255)                                                                                                                                                      aaTTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAAGAAGATAAAAATGAc                                                                                                                                                                                             <  2:41232/146‑1 (MQ=255)                                                                                                                                                            ccGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAgg                                                                              >  1:172465/1‑251 (MQ=255)                                                                                                                                                                  ccatcccACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACTTTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTAGAACGCGCTGAACTATGGCACTTCGGTTTTTGAAGGCATCCg                                                                        >  2:284649/5‑251 (MQ=255)                                                                                                                                                                  ccAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCg                                                                        <  2:34107/251‑1 (MQ=255)                                                                                                                                                                  ccAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGCGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGACGGCATCCg                                                                        >  2:7016/1‑251 (MQ=255)                                                                                                                                                                   cAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTTGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAATAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGt                                                                       <  1:151602/251‑1 (MQ=255)                                                                                                                                                                   cAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGt                                                                       >  2:156631/1‑251 (MQ=255)                                                                                                                                                                              cggtcgACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTc                                                            <  1:156631/251‑1 (MQ=255)                                                                                                                                                                                             ttAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGAGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGt                                             >  2:66331/1‑251 (MQ=255)                                                                                                                                                                                                  cAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAt                                        <  1:85773/251‑1 (MQ=255)                                                                                                                                                                                                                 gtggCCGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGc                         >  2:177710/1‑251 (MQ=255)                                                                                                                                                                                                                 gtggAAGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACCAATCCGAGCCTGAGCGCAAAAGGAATATAAAAATGACCACGCAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGAGAAGGTGCATGTGATGTCGCACGCCCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTTCGCCATCGTgcgc                         >  2:281331/1‑248 (MQ=255)                                                                                                                                                                                                                    gACGTCGCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCat                       >  1:64689/1‑250 (MQ=255)                                                                                                                                                                                                                          gCACACGTTGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGc                >  1:226287/1‑251 (MQ=255)                                                                                                                                                                                                                              acGTTGCCATCTGCCAGAGCACAACTACATCAAAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATGTGGTTCAATGGTGAGATGGTTCGTTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTGTTTGAAGGCATCCGTTGCTACGACGCGCTCAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCt            <  2:172465/251‑1 (MQ=255)                                                                                                                                                                                                                               cGTNGCCATCTGCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTg           <  2:260503/251‑1 (MQ=255)                                                                                                                                                                                                                                          gCCAGAGCACAACTACATCACAACAAATCCGCGCCTGAGAGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTcc  <  1:66331/249‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            AATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCC  >  NC_000913/3952194‑3952675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑