Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	421,341	C→T	P119L (CCG→CTG)	proY →	putative transporter ProY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	421,341	0	C	T	100.0%	104.4 / NA	31	P119L (CCG→CTG)	proY	putative transporter ProY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/11);  total (20/11)

TCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCAT  >  NC_000913/421095‑421587                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       tCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGAc                                                                                                                                                                                                                                                     <  2:92777/250‑1 (MQ=255) tCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACg                                                                                                                                                                                                                                                    >  1:52415/1‑251 (MQ=255)             gTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGTTTTGATATCCTTATTGTCGCCATCGCCGTGGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTgg                                                                                                                                                                                                                                        <  1:26533/251‑1 (MQ=255)                            tgGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAgg                                                                                                                                                                                                                         >  2:200426/1‑250 (MQ=255)                                              gtATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGGACATAACCCGGCCGACAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCACGCTGGCAGGGTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACGGGATTTGGGTACTGAGCGTGGTGCTGATCATTTgc                                                                                                                                                                                                       >  1:231653/1‑251 (MQ=255)                                                     ggcgTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACTGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGCGACCGCTTTTGGTCTCTATATTGGCTTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTTCGCAGTa                                                                                                                                                                                                 >  1:177159/1‑250 (MQ=255)                                                         tataTCCTTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCCTCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCt                                                                                                                                                                                            >  1:16119/1‑251 (MQ=255)                                                                      gtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTGTGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAACGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGATGTCTGGTTCCTGACGGGGCCGCACTGGATTTGGGAACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGa                                                                                                                                                                                 >  2:84228/1‑249 (MQ=255)                                                                            tGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTaaa                                                                                                                                                                                               <  2:266019/229‑1 (MQ=255)                                                                            tGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTaaa                                                                                                                                                                                               >  1:266019/1‑229 (MQ=255)                                                                              ggggAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCgg                                                                                                                                                                       >  1:77886/1‑251 (MQ=255)                                                                                ggAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTg                                                                                                                                                                     <  1:16577/251‑1 (MQ=255)                                                                                    aTGTCGGGACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCt                                                                                                                                                                 <  1:139158/251‑1 (MQ=255)                                                                                                      gccgtcAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCTGCCCGCGTGCAGGTTACATTACCGGCTGGACCTATTGCTGTGAAATCCTTATGGTCGCCAGCGCCTATGTGACCGCTTTTGGTATCTATATGGGTGTCTCGTTTCTGACGGCGCCGCACGGGATTTGGGTACTGAGCTTGGTGCTGATCATTTGCGCCGTAAACAGGATGAGTGTGAAGGTATTTGGTGAGGTGGCATTCTGGTTCTTGtt                                                                                                                                               <  2:231653/251‑1 (MQ=255)                                                                                                              ctctTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTaa                                                                                                                                         >  1:102581/1‑249 (MQ=255)                                                                                                                         gTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACcatc                                                                                                                            >  2:59905/1‑251 (MQ=255)                                                                                                                               cgcAGGAAACCCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAATCCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACcatcatcatc                                                                                                                      <  1:62548/251‑1 (MQ=255)                                                                                                                                     aaaaCCTCGGCCAGCTGGCAGGTTACATTACCGGCTGGACCTACTGTTTTGAAATCCTGATTGTCGCAATCGCCGATGTGAACGCTTTTGGTATCTATATTGATGTCTGGTTCATGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGATGGTATTCGGTGCGCTGGCATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGAtt                                                                                                                >  1:183432/1‑251 (MQ=255)                                                                                                                                            cGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCg                                                                                                         >  1:17978/1‑251 (MQ=255)                                                                                                                                            cGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGGGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGGATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCg                                                                                                         >  2:141673/1‑251 (MQ=255)                                                                                                                                                ccGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGttt                                                                                                     <  1:59905/251‑1 (MQ=255)                                                                                                                                                                         ggACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCCTCATCCTCTGGGGGATTGGc                                                                            >  2:32002/1‑251 (MQ=255)                                                                                                                                                                         ggACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATCTGACCGCTTTTAGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCAATGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGGTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGc                                                                            >  1:16259/1‑251 (MQ=255)                                                                                                                                                                                                    tCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCAt                                                 >  1:101977/1‑251 (MQ=255)                                                                                                                                                                                                           cGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCtg                                           <  1:47638/250‑1 (MQ=255)                                                                                                                                                                                                                atttgACCGCTTTTGGTATCTATATGGGTGTCTGGGTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGTAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGTCATCATCATCTGGGGGATTGGCAACGGCGGGGAACCGACCGGGTTTCATAAACTGTGGAgg                                     >  2:147181/4‑250 (MQ=255)                                                                                                                                                                                                                aTGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGTGCACCCGACCGGTATTCATAACCTGTGGAgg                                     >  2:216804/1‑250 (MQ=255)                                                                                                                                                                                                                       cGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAAcggcg                              <  1:200426/251‑1 (MQ=255)                                                                                                                                                                                                                       cGCTTTTGGTATCTATATGGGTGTCTGGTTCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCGGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAAcggcg                              <  1:32002/251‑1 (MQ=255)                                                                                                                                                                                                                                            tgtCTGGTTCCTGACGGTGCCGACCTGGATTTGGGTAATGAGCGTGGGGCTGTACATTTGCGCCGTAAACCTGATGAGCGTGCAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTACAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGGAACAGCCCGGTATTTACAACCTGTGGAGCAACGGCGGgttctttgagtatggtgtgg         >  2:51912/1‑237 (MQ=255)                                                                                                                                                                                                                                                   ttCCTGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCtttgcct  >  2:14627/1‑246 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       TCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCAT  >  NC_000913/421095‑421587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑