Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	434,976	T→C	I110I (ATT→ATC)	ribE →	6,7‑dimethyl‑8‑ribityllumazine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	434,976	0	T	C	100.0%	76.2 / NA	25	I110I (ATT→ATC)	ribE	6,7‑dimethyl‑8‑ribityllumazine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/10);  total (15/10)

TGACGCACTGAAACGTATCGGTCAGGTAAAAGATGAAAACATTACCGTTGTTTGGGTGCCTGGTGCCTATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTG  >  NC_000913/434751‑435173                                                                                                                                                                                                                                  |                                                                                                                                                                                                      tGACGCACTGAAACGTATCGGTCAGGTAAAAGATGAAAACATTACCGTTGTTTGGGTGCCTGGTGCCTATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGAcc                                                                                                                                                                               <  2:74360/250‑1 (MQ=255)       aCTGAAACGTATCGGTCAGGTCAAAGATGAAAACATTACCGTTGTTTGGGTGCCTGGTGCCTATGAGCTGCCGCTGGCGGCGGGCGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTTCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGGCAGCGAAATCCCGGTTGCTTTTGGGGCTCTGACCACTGaa                                                                                                                                                                         >  2:279743/1‑250 (MQ=255)         tGAAACGTATCGGTCAGGTAAAAGATGAAAACATTACCGTTGTTTGGGTGCCTGGTGCCTATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGCCGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGAACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCAATGAAAGc                                                                                                                                                                      >  1:236836/1‑251 (MQ=255)                 aTCGGTCAGGGAAAAGATGAAAACATTGCCGTTGTTTGGGTGCCTGGTGCCGATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACa                                                                                                                                                              <  1:169049/251‑1 (MQ=255)                       cAGGTAAAAGATGAAAACATTACCGTTGTTTGGGTGCCTGGTGCCTATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGAt                                                                                                                                                        <  2:28821/251‑1 (MQ=255)                                  tGAAAACATTACCGTTGTTTGGGTGCCTGGTGCCTATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCCTGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTg                                                                                                                                             >  1:129063/1‑251 (MQ=255)                                    aaaaCATTACCGTTGTTTGGGTGCCTGGTGCCTATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGc                                                                                                                                           <  2:225550/251‑1 (MQ=255)                                     aaaCATTACCGTTGTTTGGGTGCCTGGTGCCTATGAGCTGCCGCTGGCGGCGGGGGCACTGGCTAAAACCGGTAAATACGACGCGGGGATTGCGCTGGGTACGGTTATTCGTGGGGGCACAGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCCGTTGCTTTTTGGGTTCTGACCACTGAAAACATTGAACAAGCGGTCGAAAGAGCTGGc                                                                                                                                           >  2:155670/1‑250 (MQ=255)                                         aTTACCGTTGTTTGGGTGCCTGGTGCCTATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACCGCGAAATCCCGGTTGCTTTTGGGGGTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCa                                                                                                                                       >  2:100038/1‑250 (MQ=255)                                           tACCGTTGTTTGGGTGCCTGGTGCCTATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCaa                                                                                                                                      >  1:59178/1‑249 (MQ=255)                                              cGTTGTTTGGGTGCCTGGTGCCTATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAACACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACGGCCCACTTTGAATATGTCGCTGGTGGGGCAAGCAACGGCCGGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGGTCTGACCACTGCAAGCATTGAACAAGCGATCGAACGTGCTGGGACCAAAGCTg                                                                                                                                 >  2:160768/1‑251 (MQ=255)                                               gttgttTGGGTGCCTGGTGCCTATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTgg                                                                                                                                >  1:229287/1‑251 (MQ=255)                                                             tggtgcctATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGc                                                                                                                    >  1:282201/1‑249 (MQ=255)                                                                tgcctATGAGCTGACGCTGGCGGCGGGTGCACTGGCTAAAACCGGGAAATACGACGCGGTGGTAGCGCTGGGCACGGTTATTCGTGGGGGCCCTGCCCACTTTTGATATGTCGCTGGTGGTGCAAGCAAAGGGCTTGCCCATTTTGACCAGGACACAGAAATCCCGGTTGCTTTTTGGGTTCTGGCCACTGAAAGCATTCAACAAGCGATCGAACGGGCTGGCACCAAAACTGGCACCAAAAGTGCAgc                                                                                                                 >  2:196244/1‑248 (MQ=255)                                                                   ctATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCCAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGa                                                                                                                                                         >  2:178631/1‑206 (MQ=255)                                                                   ctATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGa                                                                                                                                                         <  1:178631/206‑1 (MQ=255)                                                                                  ggcggcGGGGGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCtt                                                                                              <  1:164694/250‑1 (MQ=255)                                                                                                     aaaaCCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGa                                                                           >  1:235953/1‑250 (MQ=255)                                                                                                                aTACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAgg                                                               <  1:238781/251‑1 (MQ=255)                                                                                                                    gacgCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTg                                                           <  2:186606/251‑1 (MQ=255)                                                                                                                    gacgCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTg                                                           <  1:71800/251‑1 (MQ=255)                                                                                                                                    tGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGa                                            >  1:83625/1‑250 (MQ=255)                                                                                                                                           ggTTATTCGTGGTGGCCCTGCCCACTTTGAATAGGGCGCTGGTGTTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGAAAGCGAAAGCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGAACCAAAGCTGGGATCAAAGGTGCAGAAGCTGCACTGACCGCACGTGAAATGATTAATGTAATGAAAGCCATCAAGGCCTGAAATTAGCAAGGGGAAATCCggg                                    <  2:181620/251‑3 (MQ=255)                                                                                                                                                                    tttGAATATGTCGCTGGTGGTGCCAGCACCGGCCTGGCGCATGTTGCCCAGGACAGCGCAATCCCGGTTGCTTTTGGGGGTCTAACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTTGTAAGGAGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTc           >  2:132527/1‑251 (MQ=255)                                                                                                                                                                             gTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATCCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGtgtg  >  1:202891/1‑251 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                      TGACGCACTGAAACGTATCGGTCAGGTAAAAGATGAAAACATTACCGTTGTTTGGGTGCCTGGTGCCTATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGTACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTG  >  NC_000913/434751‑435173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑