Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,325,701	G→A	A412V (GCT→GTT)	ftsH ←	ATP‑dependent zinc metalloprotease FtsH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,325,701	0	G	A	100.0%	203.3 / NA	58	A412V (GCT→GTT)	ftsH	ATP‑dependent zinc metalloprotease FtsH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/24);  total (34/24)

AATATCGTTGGACGCACCGGTAGATACATGTTCCGGCCCGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAGCCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGAGTACCACGGGCAATGATTGCCGCGTCGATATCGGGTGCCAATGGTACGCGACGCATGTGAACTTTCAGG  >  NC_000913/3325457‑3325946                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aaTATCGTTGGACGCACCGGTAGATACATGTTCCGGCCCGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTc                                                                                                                                                                                                                                                  <  2:101900/250‑1 (MQ=255)      cGTTGGACGCACCGGTAGATACATGTTCCGGCCCGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGAttct                                                                                                                                                                                                                                            <  1:19822/251‑1 (MQ=255)             cGCACCGGTAGATACATGTTCCGGCCCGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCGCTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTgcg                                                                                                                                                                                                                                     <  1:267904/251‑1 (MQ=255)             cGCACCGGTAGATACATGTTCCGGCCCGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTgcg                                                                                                                                                                                                                                     >  1:49390/1‑251 (MQ=255)                           cATGTTCCGGCCCGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTTGATTCTTTCTGCGCTTCCGTCATCAcc                                                                                                                                                                                                                       >  1:258919/1‑251 (MQ=255)                            aTGTTCCGGCCCGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGCATCTTTCTGCGCTTCCCGCATCAcc                                                                                                                                                                                                                       >  2:228355/1‑250 (MQ=255)                                 ccGGCCCGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGa                                                                                                                                                                                                                  <  1:150659/250‑1 (MQ=255)                                  cGGCCCGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGCAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACACGCTTCGTGGAAAACCCTCGTTTCTTTCTGCGCTTCAGTCATCACCTTGGaac                                                                                                                                                                                                                >  1:270168/1‑249 (MQ=255)                                  cGGCCCGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGc                                                                                                                                                                                                                >  1:34848/1‑251 (MQ=255)                                   ggCCCGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCg                                                                                                                                                                                                               >  1:249498/1‑251 (MQ=255)                                   ggCCCGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAAAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCCCTTCCGTCATCACCATGGTGCg                                                                                                                                                                                                               >  2:55713/1‑251 (MQ=255)                                     cccGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGAGCTTCCGTCATCACCATTGAGCGAc                                                                                                                                                                                                             >  1:9238/1‑251 (MQ=255)                                        gTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGGCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGtt                                                                                                                                                                                                          <  1:13310/251‑1 (MQ=255)                                                tctcTTCTGCCAGAAGACCACCGTACAGCGAGGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCAccc                                                                                                                                                                                                  >  1:247969/1‑251 (MQ=255)                                                         ccAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGAtt                                                                                                                                                                                         <  2:208454/251‑1 (MQ=255)                                                               gacCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCCTCATGATTTTGTCt                                                                                                                                                                                   >  1:260441/1‑251 (MQ=255)                                                                     cGTACAGCGTAGAAATCTGGTTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCGCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCt                                                                                                                                                                             <  2:187693/251‑1 (MQ=255)                                                                      gTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCtt                                                                                                                                                                            >  2:178633/1‑251 (MQ=255)                                                                      gTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCtt                                                                                                                                                                            >  1:124904/1‑251 (MQ=255)                                                                       tACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTAGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCTCTTTCTGCACCGGAGCGTGTTCCGGCACCAGTCGTCCGCTAACCGCATTACCCCCTTCGTGGTAAACCGTCAATTCGTTCTGAGCTTCCGTCCTCACCATGTCGCGACGTTCCGCCCCCAGCATGCTTTTGTCTTTCCCttt                                                                                                                                                                           >  2:103644/1‑251 (MQ=255)                                                                        aCAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCttt                                                                                                                                                                           <  2:198975/250‑1 (MQ=255)                                                                            cGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGa                                                                                                                                                                      <  1:178633/251‑1 (MQ=255)                                                                                gAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTCCTCGAACTc                                                                                                                                                                  <  1:63252/251‑1 (MQ=255)                                                                                  aaTCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGTCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGGCTTTACCTTTCTCGACCTCa                                                                                                                                                                 >  2:77750/1‑250 (MQ=255)                                                                                   agcTGGCTTTCCAGTTTCTTGCGGCTGGCGCTGATTGCGGCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCCCGACCGCGTGGGATAATCGTCACTTTGTGCACCTGGTCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACTCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCTCCTTGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAAc                                                                                                                                                               <  1:28409/249‑1 (MQ=255)                                                                                      tGGCTTTCCAGTTTCTGACGGCTGGCGCCGATTGCGTCGCACTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGATTTCTCGAACTCACCCAt                                                                                                                                                            >  2:186624/1‑251 (MQ=255)                                                                                      tGGCTTTCCAGTTTCTGACGGCTGGAGCAAATTGCGACGCGCTCAGGCAAGAAGAAAGGCACACCCTGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGGTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTAAATCTGCGCTTCCGTCATCACCATGGAGAGGCGTTCCGCACCCAACATGATTTTGTCTTTCGCTTTCTCGAACTCCACCAt                                                                                                                                                            >  2:206755/1‑251 (MQ=255)                                                                                                      gACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCTAACTCAACCATCGACACAACGCGt                                                                                                                                               <  2:30513/248‑1 (MQ=255)                                                                                                             ggtgcTGATTGCGTCGTCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACGTTGTTCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCAGCGACACAACGCGTTTGTTGCCa                                                                                                                                      <  1:159356/247‑1 (MQ=255)                                                                                                                      tGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCCCCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCg                                                                                                                             <  2:219820/250‑1 (MQ=255)                                                                                                                            gCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCAGGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTg                                                                                                                      <  2:249498/251‑1 (MQ=255)                                                                                                                              ccTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGcc                                                                                                                    >  2:56678/1‑251 (MQ=255)                                                                                                                               cTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCg                                                                                                                   >  1:21499/1‑251 (MQ=255)                                                                                                                                     cAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGCCCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGAGCCGCTTCGt                                                                                                             >  2:274551/1‑251 (MQ=255)                                                                                                                                                   cacCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGcc                                                                                               >  2:115093/1‑251 (MQ=255)                                                                                                                                                        aGCGCACGCCCGCGTGGGATAATCGTCTCTCTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCCCCCACCATGATTTTGTCTTTCGCTTTCTCGAACGCAACCATCTACACAACGCGTTTGTTGCCACGAGCATCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTc                                                                                          <  1:108752/251‑1 (MQ=255)                                                                                                                                                               gACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCg                                                                                   >  1:24665/1‑251 (MQ=255)                                                                                                                                                                   gngTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGga                                                                                 <  2:262749/249‑1 (MQ=255)                                                                                                                                                                   gcgTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGga                                                                                 >  1:262749/1‑249 (MQ=255)                                                                                                                                                                   gcgTGGGATAATCGTCACTGTGTGCTCCGTATTGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCCTCACCATTGTGCGACGTTCCGCACCCATCATGATTTTGTCCTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGATCAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGgaga                                                                               <  2:271663/251‑1 (MQ=255)                                                                                                                                                                    cgTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGaa                                                                              <  2:56171/251‑1 (MQ=255)                                                                                                                                                                      tGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATACTCGCATGACCCGCTTCGTGGTAAACCGTAGATTCTTTCTGCGCTTCCGTCATCCCCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCCGGTTCGCCAGGTCAGCACCGGAGAAAc                                                                            >  1:255723/1‑251 (MQ=255)                                                                                                                                                                          aTAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAgg                                                                        >  1:177740/1‑251 (MQ=255)                                                                                                                                                                                   aCTTTGTGCACCGCATCGCGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGATTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACAATGGAGCGACCTTCCGCACCCATCATGATTATGTCTTTCGCTTTCTCGCACTAAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACAGGCGAAACCAGGAGTACNACg                                                               >  2:260602/1‑251 (MQ=255)                                                                                                                                                                                           cACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCca                                                                                                                                                                                                 >  1:257875/1‑113 (MQ=255)                                                                                                                                                                                           cACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCGGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCca                                                                                                                                                                                                 <  2:257875/113‑1 (MQ=255)                                                                                                                                                                                             ccGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGAGTACCACGGGCAATGAtt                                                     <  1:104042/251‑1 (MQ=255)                                                                                                                                                                                                      gtTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGAGTACCACGGGCAATGATTGCCGCGTCg                                            >  1:27411/1‑251 (MQ=255)                                                                                                                                                                                                                cAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGAGTACCACGGGCAATGATTGCCGCGTCGATATCGGGTg                                  >  2:110517/1‑251 (MQ=255)                                                                                                                                                                                                                  ggCGACCGATAATCGCATGACCCGCTTCGTTGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCCCCATGGAGTGTCGTTCCGCTCCCATCAGGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCCCGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGATTACCACGGTCAATGATTGCCGCGTCGATATCGGGTGcc                                <  1:206755/251‑1 (MQ=255)                                                                                                                                                                                                                        cGATAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGAGTACCACGGGCAATGATTGCCGCGTCGATATCGGGTGCCAATGGt                          <  2:34848/251‑1 (MQ=255)                                                                                                                                                                                                                          aTAATCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGAGTACCACGGGCAATGATTGCCGCGTCGATATCGGGTGCCAATgnaac                        >  2:257910/1‑247 (MQ=255)                                                                                                                                                                                                                            aaTCGCATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGAGTACCACGGGCAATGATTGCCGCGTCGATATCGGGTGCCAATGGTAcgc                      <  1:193181/251‑1 (MQ=255)                                                                                                                                                                                                                                 cATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGAGTACCACGGGCAATGATTGCCGCGTCGATATCGGGTGCCAATGGTACGCGACGc                 >  2:185120/1‑251 (MQ=255)                                                                                                                                                                                                                                 cATGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGAGTACCACGGGCAATGATTGCCGCGTCGATATCGGGTGCCAATGGTACGCGACGc                 >  2:162042/1‑251 (MQ=255)                                                                                                                                                                                                                                  aTGACCCGCTTCGTGGTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGCCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGAGTACCACGGGCAATGATTGCCGCGTCGATATCGGGGGCCAATGGTACGCGACGc                 >  1:51021/1‑250 (MQ=255)                                                                                                                                                                                                                                        cGCTTCGTGGTAAAACGTCGATTCTTTATGCGCTTACGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGAGTACCACGGGCAATGATTGCCGCGTCGATATCGGGTGCCAATGGTACGCGACGCATGNGa           >  2:254119/1‑250 (MQ=255)                                                                                                                                                                                                                                                 gTAAACCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGAGTACCACGGGCAATGATTGCCGCGTCGATATCGGGTGCCAATGGTACGCGACGCATGTGAACTTTCAgg  >  1:187083/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      AATATCGTTGGACGCACCGGTAGATACATGTTCCGGCCCGTAGATGATCTCTTCTGCCAGACGACCACCGTACAGCGTAGAAATCTGGCTTTCCAGTTTCTGACGGCTGGCGCTGATTGCGTCGCCCTCAGGCAAGAAGAAAGTCACACCCAGCGCACGACCGCGTGGGATAATCGTCACTTTGTGCACCGGATCGTGTTCCGGCACCAGGCGACCGATAATCGCATGACCCGCTTCGTGGTAAGCCGTCGATTCTTTCTGCGCTTCCGTCATCACCATGGAGCGACGTTCCGCACCCATCATGATTTTGTCTTTCGCTTTCTCGAACTCAACCATCGACACAACGCGTTTGTTGCCACGAGCAGCGAACAGTGCCGCTTCGTTCACCAGGTTCGCCAGGTCAGCACCGGAGAAACCAGGAGTACCACGGGCAATGATTGCCGCGTCGATATCGGGTGCCAATGGTACGCGACGCATGTGAACTTTCAGG  >  NC_000913/3325457‑3325946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑