Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,636,644	C→T	R238H (CGT→CAT)	der ←	50S ribosomal subunit stability factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,636,644	0	C	T	100.0%	109.7 / NA	31	R238H (CGT→CAT)	der	50S ribosomal subunit stability factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/13);  total (18/13)

TGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCACGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGATCGGCAGACTTTGCGGGTCGAAGTCGTCTTCCTCTTCTTCTTCGCCGTTCTCTTCCGCTTCAAATTGCGCCCAGTATTCAGCGTCTTCGTCGACTTCCTCTTGCGGTGCGAGATCTTCCATCCACGG  >  NC_000913/2636405‑2636876                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         tGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGca                                                                                                                                                                                                                                >  1:273105/1‑249 (MQ=255)     aaGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGc                                                                                                                                                                                                                            >  1:168649/1‑250 (MQ=255)                cACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAaaca                                                                                                                                                                                                                <  1:92052/251‑1 (MQ=255)                    aTTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAaacaaca                                                                                                                                                                                                             >  1:271962/1‑250 (MQ=255)                        agaATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAAcgcg                                                                                                                                                                                                        <  2:23097/251‑1 (MQ=255)                          aaTAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGct                                                                                                                                                                                                      >  1:92024/1‑251 (MQ=255)                               aGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCa                                                                                                                                                                                                 <  1:243838/251‑1 (MQ=255)                               aGCCCAGCAGCGAGAGATCCTGAGCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTc                                                                                                                                                                                                  >  2:151446/1‑250 (MQ=255)                                gCCCAGCAGCTAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCAc                                                                                                                                                                                                <  2:126371/251‑1 (MQ=255)                                                     aTCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTagtgagtg                                                                                                                                                                           >  1:229661/1‑251 (MQ=255)                                                                       cgcATCAATCTCGAACATCACCACTGTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGGAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCg                                                                                                                                                         <  2:122127/251‑1 (MQ=255)                                                                        gcATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACATCGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCgg                                                                                                                                                        <  2:70780/251‑1 (MQ=255)                                                                            caatcaCTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGgacg                                                                                                                                                     <  1:226546/250‑1 (MQ=255)                                                                                 aCTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGGTAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTTCGTTCGGACGACCcac                                                                                                                                               <  1:252442/251‑1 (MQ=255)                                                                                   tAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCcaca                                                                                                                                              >  1:279072/1‑250 (MQ=255)                                                                                    aaCATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGAccctca                                                                                                                                              <  2:196243/249‑4 (MQ=255)                                                                                       aTCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACTCCCGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTTGAGAGTGTAGACTTACCTACGTTCGGACGCCCCACAAtagc                                                                                                                                         >  2:3996/1‑248 (MQ=255)                                                                                                         tCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGATCGGCAGACt                                                                                                                       >  2:232787/1‑251 (MQ=255)                                                                                                              ggCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGATCGGCAGACTTTGCg                                                                                                                  >  2:12145/1‑251 (MQ=255)                                                                                                                 cTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGATCGGCAGACTTTGCGGGt                                                                                                               >  1:49880/1‑251 (MQ=255)                                                                                                                  tgcaacgtcctcaTTACGGAGAATTTCTCTACAGCCTCGGTGATTTTCCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACCTCCATCGCGCTCCATTGGGCTGTAGATGCTGTCATGCGTAGTGCCAGGCATGTCGTAAACAACAACGCGATCTTCACCAAGAATACGGTTAGTGAGTGTCGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGAGCGGCAGACTTTGCGGGTc                                                                                                              >  2:157534/13‑251 (MQ=255)                                                                                                                  tGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGATCGGCAGACTTTGCGGGTc                                                                                                              >  1:170270/1‑251 (MQ=255)                                                                                                                        gTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGATCGGCAGACTTTGCGGGTCGAAgtc                                                                                                        >  1:87253/1‑251 (MQ=255)                                                                                                                                              ctACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTTGGACGACCCACAATCGCCAGTTTGATCGGCAGACTTTGCGGGTCGAAGTCGTCTTCCTCTTCTTCTTCGCCg                                                                                  >  1:179877/1‑251 (MQ=255)                                                                                                                                                aCAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGATCGGCAGACTTTGCGGGTCGAAGTCGTCTTCCTCTTCTTCTTCGCCGtt                                                                                <  1:248260/251‑1 (MQ=255)                                                                                                                                                aCAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGGCGTGCCAGGCATGTCGTAAATAACAACGCGCTCTTCACCAAGAATATGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGATTGATCGGCAGACTTTACGGTTCGACGTCGACTTCCTCATCTTCTTCGCCGtt                                                                                >  2:235317/1‑251 (MQ=255)                                                                                                                                                            aTTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTATATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGATCGGCAGACTTTGCGGGTCGAAGTCGTCTTCGTCTTCTTCTTCGCCGTTCTCTTCCGCTTc                                                                    <  1:222317/251‑1 (MQ=255)                                                                                                                                                                  ccGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGATCGGCAGACTTTGCGGGTCGAAGTCGTCTTCCTCTTCTTCTTCGCCGTTCTCTTCCGCTTCAANTTg                                                              >  2:255810/1‑251 (MQ=255)                                                                                                                                                                                                             gTCCATCGCGTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTGAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGATCAGCAGACTTTGCGGGTCGAAGTCGTCTTCCTCTTCTTCTTCGCCGTTCTCTTCCGCTTCAAATTGCGCCCAGTATTCAGCGTCTTCGTCGACTTCCTCTTGCGGcgcg                   >  1:253312/1‑251 (MQ=255)                                                                                                                                                                                                                   cgcgTTCCATTGGGATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGATCGGCAGACTTTGCGGGTCGAAGTCGTCTTCCTCTTCTTCTTCGCCGTTCTCTTCCGCTTCAAATTGCGCCCAGTATTCAGCGTCTTCGTCGACTTCCTCTTGCGGTGCGAGATc              <  2:96708/250‑1 (MQ=255)                                                                                                                                                                                                                              gggATGTAGATGCTGTCATGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGATCGGCAGACTTTGCGGGTCGAAGTCGTCTTCCTCTTCTTCTTCGCCGTTCTCTTCCGCTTCAAATTGCGCCCAGTATTCAGCGTCTTCGTCGACTTCCTCTTGCGGTGCGAGATCTTCCATCCACgg  <  1:154994/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         TGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGTGTCAATGAGCACATACTCACGTCCATCGCGTTCCATTGGGATGTAGATGCTGTCACGCGTCGTGCCAGGCATGTCGTAAACAACAACGCGCTCTTCACCAAGAATACGGTTAGTGAGTGTAGACTTACCTACGTTCGGACGACCCACAATCGCCAGTTTGATCGGCAGACTTTGCGGGTCGAAGTCGTCTTCCTCTTCTTCTTCGCCGTTCTCTTCCGCTTCAAATTGCGCCCAGTATTCAGCGTCTTCGTCGACTTCCTCTTGCGGTGCGAGATCTTCCATCCACGG  >  NC_000913/2636405‑2636876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑