Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,555,053	C→T	A44T (GCT→ACT)	eutR ←	putative DNA‑binding transcriptional regulator EutR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,555,053	0	C	T	100.0%	83.6 / NA	25	A44T (GCT→ACT)	eutR	putative DNA‑binding transcriptional regulator EutR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/14);  total (11/14)

GATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGC  >  NC_000913/2554823‑2555299                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       gATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTtg                                                                                                                                                                                                                                    <  2:199526/251‑1 (MQ=255)         cgcTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGt                                                                                                                                                                                                                            <  1:274510/251‑1 (MQ=255)                    cATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACAtt                                                                                                                                                                                                                 <  1:82122/251‑1 (MQ=255)                       tGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTAt                                                                                                                                                                                                               <  1:60755/250‑1 (MQ=255)                           aaCCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACt                                                                                                                                                                                                          <  1:87875/251‑1 (MQ=255)                               gATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCg                                                                                                                                                                                                       >  1:112775/1‑250 (MQ=255)                                    aTCCCTGCTCACCGCGCGTCGCCGGAATTCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTc                                                                                                                                                                                                 <  1:46298/251‑1 (MQ=255)                                          gCTCACCGCGCGGCGCAGGAATGCAAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCAGAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTg                                                                                                                                                                                           >  2:187805/1‑251 (MQ=255)                                           cTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTgg                                                                                                                                                                                          >  1:58482/1‑250 (MQ=255)                                                   gcgTCGCCGGAATGCCAAACCAGAACGATTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCAGCGAGTAGAATTTCCGTAAATTCTCCATGAAACTTGCCCGGATGCAGCTGTTCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTAGGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCATCGGCGTGAGCTTCAgg                                                                                                                                                                                  <  2:152884/251‑1 (MQ=255)                                                                                             ggCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCt                                                                                                                                        <  1:130871/251‑1 (MQ=255)                                                                                                 cGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTAc                                                                                                                                    <  1:15217/251‑1 (MQ=255)                                                                                                           aGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCttttt                                                                                                                          >  1:103245/1‑251 (MQ=255)                                                                                                                           aTTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTGCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGTCTGTACGGGTCTTTTTCATGATGTTAATGCCg                                                                                                          <  2:188677/251‑1 (MQ=255)                                                                                                                                    aaaCCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGTTGTTGTa                                                                                                 <  1:54730/251‑1 (MQ=255)                                                                                                                                              atcatcGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCgg                                                                                       >  2:94681/1‑251 (MQ=255)                                                                                                                                                 atcGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGa                                                                                              >  1:29422/1‑241 (MQ=255)                                                                                                                                                 atcGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGa                                                                                              <  2:29422/241‑1 (MQ=255)                                                                                                                                                      gTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                               <  2:45488/251‑1 (MQ=255)                                                                                                                                                                         aCCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTga                                                            <  2:103245/251‑1 (MQ=255)                                                                                                                                                                                                   gATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGt                                  >  2:43555/1‑251 (MQ=255)                                                                                                                                                                                                                ctgcCAGGCGGTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGGAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATTTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGc                       >  1:194138/1‑249 (MQ=255)                                                                                                                                                                                                                         ggTAATCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCAACTTCGACCTTCGGCGTGAGCTTAAGATTTGCGGGTAAGGGTTCATGATAAAGAGGGTGCAAATTGGATGTACGGGTCTTTTTCATGATGTTAATGCCGGGAGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACAGCGTTTACGTAATGTCCCGGCAGAAAAGAGCt            >  1:165215/1‑251 (MQ=255)                                                                                                                                                                                                                            aaTCGTTAAAGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGtt         >  2:109664/1‑251 (MQ=255)                                                                                                                                                                                                                                   aaaGTATGTTCATAGACATCCGTTGTCTGTCGTTGATGAAAATTATCCACTTCGACAATCGGCGTGAGCTTCAGGTTTTAGGGTAAGGGATCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGGTGTAGGACACCGGGCACCTCCGACAGGTTAATGGGGCTTGAGACGAAAACGACTACTGCGTTTACGTAATGTCCCGGAAGCAAAGAGCTGTTCGAgcgc  >  2:126465/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       GATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGC  >  NC_000913/2554823‑2555299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑