Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,941,982	C→T	noncoding (175/1542 nt)	rrsC →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,941,982	0	C	T	100.0%	36.2 / NA	16	noncoding (175/1542 nt)	rrsC	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/3);  total (13/3)

AGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGA  >  NC_000913/3941794‑3942221                                                                                                                                                                                             |                                                                                                                                                                                                                                                aGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCCGATGTGccc                                                                                                                                                                                     >  1:143129/1‑249 (MQ=14)     tCAAACTTTTAAATTGCAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGGGCGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTTCTGGAAACGGTAGCTAATATCGCATAACGTTGGAAGACCCATGAGGGGGACCTTCGGGCCTCTTTCCATCAGCTGTGCCCAGATgg                                                                                                                                                                               >  2:17525/1‑251 (MQ=11)                           ttGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTa                                                                                                                                                          >  1:150582/1‑250 (MQ=37)                            tGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGAACTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGGAGGGGGGGTAAc                                                                                                                                                        >  2:167111/1‑251 (MQ=255)                              aTCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGAAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACATTCGGGCCTCTTGCCATCAGATGTGCACAGATGGGGTTAGCTAGTAGGTGGGGTAACgg                                                                                                                                                      >  2:115144/1‑251 (MQ=255)                                      tCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATTTGCCCAGATGGGATTAGCTAGTAGGTGGGGTACCGGCTCACCtt                                                                                                                                              >  2:159635/1‑250 (MQ=255)                                                             ccTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTg                                                                                                                       <  1:167111/251‑1 (MQ=255)                                                                                        aGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGCCTGGGAAACTGTTTGATGGAGGGGGATAACTACTGGAGACGGGAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGCGCCTCTTGCCCTCGGATGTGCCCCGCTGGGATTAGCTCGGATGTCGGGTAACGGCTCACCTATGCGACGATCCCTAGCCGGTCTGTGCGGATGCCCCTCCGCACTGGAACtc                                                                                            >  2:25717/1‑250 (MQ=11)                                                                                                     cTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGccc                                                                                                                                                                                     >  1:167259/1‑149 (MQ=255)                                                                                                     cTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGccc                                                                                                                                                                                     <  2:167259/149‑1 (MQ=255)                                                                                                                                        gTCTGGGAAACTGTATGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGAACCTTCGGGCCTCTTGACATAAGATGTGCACAGATGGGATTAGCTAGTATGAGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGGCCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGcac                                            <  2:225119/251‑1 (MQ=11)                                                                                                                                                   tgtcTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCa                                  >  1:218887/4‑250 (MQ=14)                                                                                                                                                            gAGGGGGATAACTACTGGCAACGGTAGATAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGc                        >  1:75532/1‑251 (MQ=14)                                                                                                                                                                              aaaCGGTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATg      >  1:163986/1‑251 (MQ=14)                                                                                                                                                                                  ggTAGCTAATATCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATga     >  1:85361/1‑248 (MQ=14)                                                                                                                                                                                   gTAGCTAATATCGCATAACGTCGCAAGAACAAAGAGGGGGCCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATgaaga  >  1:250383/1‑250 (MQ=14)                                                                                                                                                                                             |                                                                                                                                                                                                                                                AGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGA  >  NC_000913/3941794‑3942221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑