Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,403,891	G→A	M136I (ATG→ATA)	yhdH →	acrylyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,403,891	0	G	A	100.0%	95.5 / NA	31	M136I (ATG→ATA)	yhdH	acrylyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/19);  total (12/19)

CCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACA  >  NC_000913/3403645‑3404135                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     ccGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATTCTGTGTGTGATAgcg                                                                                                                                                                                                                                                   <  1:11100/250‑1 (MQ=255)           ccGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGc                                                                                                                                                                                                                                        >  1:113205/1‑251 (MQ=255)           ccGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGGGACTGGCTGGGTGCCCTGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGGACTGCCGGGTTTACCGCCATGCAGTGTGGGATAGCGCTGGAAGATGc                                                                                                                                                                                                                                        >  2:270967/1‑251 (MQ=255)              atgatTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCgg                                                                                                                                                                                                                                     >  1:80730/1‑251 (MQ=255)                   tCCTGGGAGCGATTTTGCCGTAACGGTACGCACCAGCGAAGATCCGCGTTTTCAGGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTTCCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGtt                                                                                                                                                                                                                                 <  2:117548/250‑1 (MQ=255)                       gggATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGccc                                                                                                                                                                                                                            <  1:112471/251‑1 (MQ=255)                              aTTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGAc                                                                                                                                                                                                                     >  1:120104/1‑251 (MQ=255)                                  tgcggaaaCTGTACGCTCCAGCTAACATCCGCGTTTCCATGCCGGTCAGGAGGGGTTACCCACTGGCTGGGGAGTTGGTGAAAACCACTGGGGTGGGTTGGGGGAGCAGGCGCGAGTGAAAGGGGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTAGCGGTACTGCCGGTTTTACCGCAATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCCGGACggg                                                                                                                                                                                                                  <  2:144/244‑1 (MQ=255)                                                             tCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGAATGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAgtggtg                                                                                                                                                                                      >  1:126309/1‑251 (MQ=255)                                                                gcgTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCg                                                                                                                                                                                   >  1:61517/1‑251 (MQ=255)                                                                      tCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGTGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGc                                                                                                                                                                              <  1:138504/250‑1 (MQ=255)                                                                        aTGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGt                                                                                                                                                                           <  1:217448/251‑1 (MQ=255)                                                                                 aggaggTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGGTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGGTCGCCCGCAGGACGGGGAGATTGTCGTGCCGGGGGCCAGTGGGGGCGTCGGCAGGACCGCCGTg                                                                                                                                                                  >  2:91562/1‑251 (MQ=255)                                                                                                   gctggGGCTTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGGGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTTCTTCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTg                                                                                                                                                <  1:45346/251‑1 (MQ=255)                                                                                                              ggTGAAAACCACTGGGTTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCTTGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAgg                                                                                                                                      <  1:282407/250‑1 (MQ=255)                                                                                                                               tGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGTc                                                                                                                    >  2:136012/1‑251 (MQ=255)                                                                                                                               ggggcggggggAGCGGGCGCGATTAAAGGGTAACTGGCTGTTTGCCAGCCCGCAAGGGCGTGACTCGCTTAAAGTAATGATTATCGGCACGGCTGGTTTTCCCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGTc                                                                                                                    <  2:70656/242‑1 (MQ=255)                                                                                                                                           gaaGGCGGGAGGGAAAGGTGACTGGCGGGTGGCCATGCCGCAAGGGCGGGACGCGCGTAAAGCAATGATTAGCGGTACGGCCGGTTTGACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTAccc                                                                                                        <  1:276373/249‑1 (MQ=255)                                                                                                                                               gcgcGAGTGAAAGGTGACTGGCTGGTGGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGGGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGa                                                                                                    <  1:31718/251‑1 (MQ=255)                                                                                                                                                      tGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGGTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGGTGGGTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTg                                                                                             >  1:76157/1‑251 (MQ=255)                                                                                                                                                         aaGGTGACTGGTTGGTTGCCATGCCGAAGGGGTTGGACGCGCGTAAAGCAATGATTGTCGGTCCTGCGGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGGCGCGAAAGTACCCATGAATATCTGaaa                                                                                          <  1:219162/251‑1 (MQ=255)                                                                                                                                                                         tgccttgccccaggggtgggACGCACGTAAAGAAAGGATTTTCGGTACGGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGc                                                                           <  2:42056/232‑1 (MQ=255)                                                                                                                                                                                  gCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTcc                                                                 <  1:57317/251‑1 (MQ=255)                                                                                                                                                                                            gACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCa                                                                                                                                        >  1:68759/1‑170 (MQ=255)                                                                                                                                                                                            gACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCa                                                                                                                                        <  2:68759/170‑1 (MQ=255)                                                                                                                                                                                                                    gttcatGCCGGTTTTGCCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGGGTTCGCCCGGAGAGCGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCTTCGGCAGTCCCGCCGTGGCGCTGCTGCATAAGGTGGGTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCTGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGa                               <  2:150570/246‑1 (MQ=255)                                                                                                                                                                                                                            cGGTTTTACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAg                       <  1:136012/251‑1 (MQ=255)                                                                                                                                                                                                                              gTTTTACCGCCATGCTGTGTGTGCTAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTc                     >  2:278037/1‑251 (MQ=255)                                                                                                                                                                                                                                 ttACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctg                   <  2:61517/250‑1 (MQ=255)                                                                                                                                                                                                                                  tACCGCCATGCTGTGTGTGATAGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctgg                  <  1:109487/250‑1 (MQ=255)                                                                                                                                                                                                                                                 tgtgATAGCGCTGGAAGCTGCCGGTGTTCTCCCGCAGTACGGGGAGATTGTCGTGACGCGTGCCCGTTGTGTCGTCGGCAGTACAGACGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGTCGCGACAGTCCCCATGCATATCTGAAAAGTTTAGGTGCTATCCGTGTTCTCCCTCGTGATGGGTTTGCCGAATCCCGTCCTCTGGTAAAACAACTCTCGTCTGGGGCTATTGacc  >  2:58734/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTGGCGCTGCTGCATAAGTTGGGTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACA  >  NC_000913/3403645‑3404135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑